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| 45. |  | MOREIRA, R.; VIEIRA, D.; BIASOTO, A. C. T.; FREITAS, S. T. de; SILVA, F.; SASSI, K. Perfil sensorial do consumidor de uva-passa na região de João Pessoa - PB. In: CONGRESSO INTERNACIONAL DE GASTRONOMIA E CIÊNCIA DE ALIMENTOS, 2., 2016, Fortaleza. Gastronomia: da tradição à inovação - anais. Fortaleza: UFC, 2016. p. 481-482.| Biblioteca(s): Embrapa Semiárido. |
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| 49. | | FREITAS, S. T. de; AMARANTE, C. V. T.; MITCHAM, E. J. Mechanisms regulating bitter pit development in Greensleeves apples with suppression of ethylene biosynthesis. In: CONGRESSO BRASILEIRO DE PROCESSAMENTO MÍNIMO E PÓS COLHEITA DE FRUTAS, FLORES E HORTALIÇAS, 1.; SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA, FRUTAS, HORTALIÇAS E FLORES, 5.; ENCONTRO NACIONAL SOBRE PROCESSAMENTO MÍNIMO DE FRUTAS E HORTALIÇAS, 8., 2015, Aracaju. Avanço na conservação e qualidade de frutas, flores e hortaliças: anais. Aracaju: Universidade Federal de Sergipe: Embrapa, 2015. 1 CD-ROM| Biblioteca(s): Embrapa Semiárido. |
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| 57. | | VASCONCELOS, O.; TURCO, S. H. N.; DACANAL, C.; LUZ, S.; FREITAS, S. T. de. Thermal environment of table grape packing houses in the São Francisco Valley. Engenharia Agrícola, Jaboticabal, v. 37, n. 1, p. 35-45, jan./fev. 2017.| Biblioteca(s): Embrapa Semiárido. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/07/2022 |
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Data da última atualização: |
18/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RIBOLDI, L. B.; FREITAS, S. T. de; NORRIS, A. M.; JIANG, C.-Z. |
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Afiliação: |
LUCAS BAIOCHI RIBOLDI, ESALQ; SERGIO TONETTO DE FREITAS, CPATSA; AYLA MARIE NORRIS, Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Davus, CA; CAI-ZHONG JIANG, Crops Pathology and Genetic Research Unit, United States Department of Agriculture, Davis, CA. |
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Título: |
Xylem functionality controlling blossom-end rot incidence in transgenic ALC::NCED tomato plants. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
South African Journal of Botany, v. 150, 2022. |
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DOI: |
https://doi.org/10.1016/j.sajb.2022.07.015 |
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Idioma: |
Inglês |
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Conteúdo: |
Fruit susceptibility to the physiological disorder known as blossom-end rot (BER) is an important limitation in tomato production. Abscisic acid (ABA) is known to reduce leaf transpiration, which can enhance plant water use efficiency (WUE), as well as increase fruit xylem functionality, Ca2+ uptake and oxidative stress defenses, which has been suggested to reduce BER incidence. However, the role of ABA on most of these factors determining fruit susceptibility to BER remains poorly understood. ABA production is mainly regulated by the expression of 9-cis-epoxycarotenoid dioxygenase (NCED) genes. Manipulation of NCED gene expression by the alcohol inducible promoter (ALC) could be an alternative approach to stimulate ABA production and its beneficial effects on inhibiting BER incidence. The objectives of this study were to use ALC::NCED transgenic tomato plants to decrease BER incidence and investigate which mechanisms were involved in BER. In this study, two transgenic tomato lines (1 and 2) were developed with the ALC::NCED construct. This construct allows the inducible activation of the ALC promoter by treating the plants with ethanol vapor that drives NCED expression and ABA synthesis. According to the results, after full bloom, weekly spraying transgenic plants with ethanol (2%) decreased BER incidence in both transgenic lines, compared to the wild type ?New Yorker? plants. The transgenic line 1 had higher NCED expression in response to ethanol than the transgenic line 2 and wild type ?New Yorker?. At 15 and 30 days after pollination, transgenic lines 1 and 2 had higher number of functional xylem vessels which helped to increase Ca2+ concentration in the distal end of the fruit, compared to the wild type fruit. In response to higher NCED expression, WUE and antioxidant content in leaves and fruit were higher in both transgenic lines, compared to the wild type, helping to explain the lower BER incidence. Therefore, our study shows that stimulating NCED expression with an inducible system increases the number of functional xylems and Ca2+ uptake into the fruit, improving plant WUE and reducing BER incidence MenosFruit susceptibility to the physiological disorder known as blossom-end rot (BER) is an important limitation in tomato production. Abscisic acid (ABA) is known to reduce leaf transpiration, which can enhance plant water use efficiency (WUE), as well as increase fruit xylem functionality, Ca2+ uptake and oxidative stress defenses, which has been suggested to reduce BER incidence. However, the role of ABA on most of these factors determining fruit susceptibility to BER remains poorly understood. ABA production is mainly regulated by the expression of 9-cis-epoxycarotenoid dioxygenase (NCED) genes. Manipulation of NCED gene expression by the alcohol inducible promoter (ALC) could be an alternative approach to stimulate ABA production and its beneficial effects on inhibiting BER incidence. The objectives of this study were to use ALC::NCED transgenic tomato plants to decrease BER incidence and investigate which mechanisms were involved in BER. In this study, two transgenic tomato lines (1 and 2) were developed with the ALC::NCED construct. This construct allows the inducible activation of the ALC promoter by treating the plants with ethanol vapor that drives NCED expression and ABA synthesis. According to the results, after full bloom, weekly spraying transgenic plants with ethanol (2%) decreased BER incidence in both transgenic lines, compared to the wild type ?New Yorker? plants. The transgenic line 1 had higher NCED expression in response to ethanol than the transgenic line 2... Mostrar Tudo |
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Palavras-Chave: |
Atividade antioxidante; Eficiência no uso da água; Expressão induzida por álcool. |
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Thesagro: |
Ácido Abscisico; Cálcio; Distúrbio Fisiológico; Tomate. |
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Thesaurus NAL: |
Abscisic acid; Antioxidant activity; Calcium; Tomatoes; Water use efficiency. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03098naa a2200313 a 4500
001 2144785
005 2022-07-18
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.sajb.2022.07.015$2DOI
100 1 $aRIBOLDI, L. B.
245 $aXylem functionality controlling blossom-end rot incidence in transgenic ALC$b:NCED tomato plants.$h[electronic resource]
260 $c2022
520 $aFruit susceptibility to the physiological disorder known as blossom-end rot (BER) is an important limitation in tomato production. Abscisic acid (ABA) is known to reduce leaf transpiration, which can enhance plant water use efficiency (WUE), as well as increase fruit xylem functionality, Ca2+ uptake and oxidative stress defenses, which has been suggested to reduce BER incidence. However, the role of ABA on most of these factors determining fruit susceptibility to BER remains poorly understood. ABA production is mainly regulated by the expression of 9-cis-epoxycarotenoid dioxygenase (NCED) genes. Manipulation of NCED gene expression by the alcohol inducible promoter (ALC) could be an alternative approach to stimulate ABA production and its beneficial effects on inhibiting BER incidence. The objectives of this study were to use ALC::NCED transgenic tomato plants to decrease BER incidence and investigate which mechanisms were involved in BER. In this study, two transgenic tomato lines (1 and 2) were developed with the ALC::NCED construct. This construct allows the inducible activation of the ALC promoter by treating the plants with ethanol vapor that drives NCED expression and ABA synthesis. According to the results, after full bloom, weekly spraying transgenic plants with ethanol (2%) decreased BER incidence in both transgenic lines, compared to the wild type ?New Yorker? plants. The transgenic line 1 had higher NCED expression in response to ethanol than the transgenic line 2 and wild type ?New Yorker?. At 15 and 30 days after pollination, transgenic lines 1 and 2 had higher number of functional xylem vessels which helped to increase Ca2+ concentration in the distal end of the fruit, compared to the wild type fruit. In response to higher NCED expression, WUE and antioxidant content in leaves and fruit were higher in both transgenic lines, compared to the wild type, helping to explain the lower BER incidence. Therefore, our study shows that stimulating NCED expression with an inducible system increases the number of functional xylems and Ca2+ uptake into the fruit, improving plant WUE and reducing BER incidence
650 $aAbscisic acid
650 $aAntioxidant activity
650 $aCalcium
650 $aTomatoes
650 $aWater use efficiency
650 $aÁcido Abscisico
650 $aCálcio
650 $aDistúrbio Fisiológico
650 $aTomate
653 $aAtividade antioxidante
653 $aEficiência no uso da água
653 $aExpressão induzida por álcool
700 1 $aFREITAS, S. T. de
700 1 $aNORRIS, A. M.
700 1 $aJIANG, C.-Z.
773 $tSouth African Journal of Botany$gv. 150, 2022.
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