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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/06/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. |
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Afiliação: |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
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Título: |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. |
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DOI: |
10.1021/acs.estlett.7b00456 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. MenosABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites ... Mostrar Tudo |
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Palavras-Chave: |
Biodegradação de dioxano; Microbactéria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2082755
005 2019-06-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.estlett.7b00456$2DOI
100 1 $aHE, Y.
245 $aDioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.$h[electronic resource]
260 $c2017
520 $aABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
653 $aBiodegradação de dioxano
653 $aMicrobactéria
700 1 $aMATHIEU, J.
700 1 $aYANG, Y.
700 1 $aYU, P.
700 1 $aSILVA, M. L. B. da
700 1 $aALVAREZ, P. J. J.
773 $tEnvironmental Science & Technology$gv. 4, n. 11, p. 494-499, 2017.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Tipo/Formato |
Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/03/2016 |
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Data da última atualização: |
21/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
RYBKA, A. C. P.; FREITAS, S. T. de; FIGUEIRETO NETO, A.; BIASOTO, A. C. T. |
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Afiliação: |
ANA CECILIA POLONI RYBKA, CPATSA; SERGIO TONETTO DE FREITAS, CPATSA; ACÁCIO FIGUEIREDO NETO, UNIVASF; ALINE TELLES BIASOTO MARQUES, CPATSA. |
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Título: |
Central composite rotatable design approach to optimize Italia raisin drying conditions. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Comunicata Scientiae, v. 6, n. 4, p. 454-462, 2015. |
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DOI: |
10.14295/CS.v6i4.993 |
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Idioma: |
Inglês |
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Conteúdo: |
Considering its high demand and limited production, raisin represents an important alternative to diversify grape processed products around the world. The aim of this study was to determine the best combination between drying temperature and time required to reach the highest consumer acceptance of ?Italia? raisin produced in the semi-arid climate in Brazil. The drying conditions were combinations between drying temperatures of 50, 56, 70, 84 and 90°C and drying times of 16, 22, 35, 48 and 54 hours, following a central composite rotational design (CCRD). The best combination between drying temperature and time was estimated to be 70°C for 35 hours (h), based on overall consumer acceptance. According to the statistical analysis used, drying at 70 °C for 35h and at 59 °C for 28h results in equal overall consumer acceptance, being the second option the more economical. Taste and texture, raisin chroma values and pH were more positively correlated to overall acceptance. The results indicate that drying at 59ºC for 28 h is the most efficient drying condition for raisins from ?Italia? grapes grown in Brazilian semi-arid condition. This study uses a new approach based on the central composite rotatable design to determine the most efficient drying temperature and time for Italia raisins. |
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Palavras-Chave: |
Análise sensorial; Uva passa. |
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Thesagro: |
Mercado; Secagem; Temperatura; Uva; Vitis Vinifera. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140407/1/Sergio-2015.pdf
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Marc: |
LEADER 02051naa a2200265 a 4500
001 2039114
005 2016-03-21
008 2015 bl uuuu u00u1 u #d
024 7 $a10.14295/CS.v6i4.993$2DOI
100 1 $aRYBKA, A. C. P.
245 $aCentral composite rotatable design approach to optimize Italia raisin drying conditions.$h[electronic resource]
260 $c2015
520 $aConsidering its high demand and limited production, raisin represents an important alternative to diversify grape processed products around the world. The aim of this study was to determine the best combination between drying temperature and time required to reach the highest consumer acceptance of ?Italia? raisin produced in the semi-arid climate in Brazil. The drying conditions were combinations between drying temperatures of 50, 56, 70, 84 and 90°C and drying times of 16, 22, 35, 48 and 54 hours, following a central composite rotational design (CCRD). The best combination between drying temperature and time was estimated to be 70°C for 35 hours (h), based on overall consumer acceptance. According to the statistical analysis used, drying at 70 °C for 35h and at 59 °C for 28h results in equal overall consumer acceptance, being the second option the more economical. Taste and texture, raisin chroma values and pH were more positively correlated to overall acceptance. The results indicate that drying at 59ºC for 28 h is the most efficient drying condition for raisins from ?Italia? grapes grown in Brazilian semi-arid condition. This study uses a new approach based on the central composite rotatable design to determine the most efficient drying temperature and time for Italia raisins.
650 $aGrapes
650 $aMercado
650 $aSecagem
650 $aTemperatura
650 $aUva
650 $aVitis Vinifera
653 $aAnálise sensorial
653 $aUva passa
700 1 $aFREITAS, S. T. de
700 1 $aFIGUEIRETO NETO, A.
700 1 $aBIASOTO, A. C. T.
773 $tComunicata Scientiae$gv. 6, n. 4, p. 454-462, 2015.
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Embrapa Semiárido (CPATSA) |
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