Data/hora: 17/06/2024 23:56:44

Biblioteca(s): | Embrapa Café.
Data corrente: | 22/02/2017
Data da última atualização: | 03/03/2017
Autoria: | FREITAS, R. T. da; PAIVA, R.; SALES, T. S.; SILVA, D. P. C. da; REIS, M. V. dos; SOUZA, A. C. de; ROSA, S. D. V. F. da.
Afiliação: | RODRIGO THEREZAN DE FREITAS, UFLA; RENATO PAIVA, UFLA; THAIS SILVA SALES, UFLA; DIOGO PEDROSA CORRÊA DA SILVA, UFLA; MICHELE VALQUÍRIA DOS REIS, UFLA; ANA CRISTINA DE SOUZA, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC.
Título: | Cryopreservation of Coffea arabica L. zygotic embryos by vitrification.
Ano de publicação: | 2016
Fonte/Imprenta: | Notulac Botanicae Horti Agrobotanici Cluj-Napoca, v. 44, n. 2, p. 445-451, Dec. 2016.
Idioma: | Inglês
Conteúdo: | As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.
Palavras-Chave: | Liquid nitrogen; PVS2; TTC test.
Thesaurus Nal: | Ex situ conservation; Histology; Thawing.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/156422/1/Cryopreservation-of-Coffea-arabica.pdf
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1075-1) - UPC - AP - DD -  -  -  - DIS