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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
25/11/2015 |
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Data da última atualização: |
04/03/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
LOUZAN, A. L. R. M.; CAITANO, M. A. B.; SANTOS, M. G. dos; VERBISCK, N. V.; ROSINHA, G. M. S. |
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Afiliação: |
UFMS; UFMS; MARIA GORETTI DOS SANTOS, CNPGC; NEWTON VALERIO VERBISCK, CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
Maldi-tof mass spectrometry identification and differenteiation of Brucella species. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61. |
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Idioma: |
Inglês |
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Conteúdo: |
Brucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES. MenosBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied he... Mostrar Tudo |
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Thesagro: |
Brucella. |
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Thesaurus Nal: |
Brucellosis; Spectroscopy. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02467nam a2200193 a 4500
001 2029543
005 2016-03-04
008 2015 bl uuuu u00u1 u #d
100 1 $aLOUZAN, A. L. R. M.
245 $aMaldi-tof mass spectrometry identification and differenteiation of Brucella species.$h[electronic resource]
260 $aIn: MEDITERRANEAN SEA REGION COUNTRIES MASS SPECTROMETRY WORKSHOP (MEDMS III), 3., 2015., Athens, Greece. [Poster]. Athens, Greece: Univeristy of Athens, 2015. p. 61.$c2015
520 $aBrucella species are Gram-negative bacteria that cause BrucelIosis, also known as Mediterranean or Malta fever, a highly contagious and chronic disease that occurs worldwide. Brucellosis may affect human after close contact with infected animal secretions and by ingestion of contaminated animal products, such as those made with unpausterized milk or undercooked meat. Livestock, including cattle, sheep, goats, pigs and dogs are the most common reservoirs for Brucella bacteria, which the detection stilI relies on microbiological culture and serological tests. However, in the last decade, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) has been used for microrganism identification at genus and species leveI. CurrentIy, MALDI-TOF MS is being used as a rapid, cost effective and reliable methodology for bacteria identification even at the subspecies leveI. Here we show that MALDI- TOF MS is useful for Brucella identification and differentiation, B. abortus (strains 544, S2308, 19 and RB51), B. suis, B. ovis and B. canis reference samples were grown and, after ethanol inactivation, proteins were extracted with formic acidlacetonitrile. Mass spectra were acquired with alpha-cyano-4-hydroxy cinnamic acid in an Autoflex III Smartbeam mass spectrometer (Bruker Daltonics). MALDI Biotyper software (Bruker Daltonics) dendogram analysis provided that the mass spectra profiles (MSPs) obtained can distinguish alI the species of Brucella studied herein. Moreover, fingerprints of the wild-type S2308 and vaccine 19 and RB51 B. abortus strains are clearly different. The next step wilI be to use these MSPs to detect and identify Brucella from infected cattle. Financial support: Embrapa, FUNDECT, CNPq and CAPES.
650 $aBrucellosis
650 $aSpectroscopy
650 $aBrucella
700 1 $aCAITANO, M. A. B.
700 1 $aSANTOS, M. G. dos
700 1 $aVERBISCK, N. V.
700 1 $aROSINHA, G. M. S.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Solos. |
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Data corrente: |
06/12/2005 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
BERNARDI, A. C. de C.; CARVALHO, M. da C. S.; FREITAS, P. L. de; OLIVEIRA JUNIOR, J. P.; LEANDRO, W. M.; SILVA, T. M. da. |
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Afiliação: |
ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; MARIA DA CONCEICAO SANTANA CARVALHO, CNPAF; PEDRO LUIZ DE FREITAS, CNPS; JUAREZ PATRICIO DE OLIVEIRA JUNIOR, UFG; WILSON MOZEMNA LEANDRO, UFG; THIAGO MESQUITA DA SILVA, UFG. |
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Título: |
No sistema plantio direto é possível antecipar a adubação do algodoeiro. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Rio de Janeiro: Embrapa Solos, 2004. |
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Páginas: |
7 p. |
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Série: |
(Embrapa Solos. Comunicado Técnico, 24). |
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ISSN: |
1517-5685 |
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Idioma: |
Português |
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Conteúdo: |
O SPD vem sendo adotado na região dos Cerrados, prinmcipalmente como forma de evitar os problemas de erosão e compactação do solo. A adoção pelo cotonicultores de Goiás mostyra qye é uma alternativa de produção viável e sustentável, e tem demostrado que pode vis a subistituir o sistema convencional. |
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Palavras-Chave: |
Algodoeiro; Sistema de plantio direto; Sistema plantio direto. |
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Thesagro: |
Adubação; Algodão; Plantio Direto. |
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Categoria do assunto: |
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P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147792/1/CNPS-CT24-Adubacao-do-Algodeiro.pdf
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Marc: |
LEADER 01104nam a2200277 a 4500
001 1047247
005 2023-03-09
008 2004 bl uuuu u0uu1 u #d
022 $a1517-5685
100 1 $aBERNARDI, A. C. de C.
245 $aNo sistema plantio direto é possível antecipar a adubação do algodoeiro.
260 $aRio de Janeiro: Embrapa Solos$c2004
300 $a7 p.
490 $a(Embrapa Solos. Comunicado Técnico, 24).
520 $aO SPD vem sendo adotado na região dos Cerrados, prinmcipalmente como forma de evitar os problemas de erosão e compactação do solo. A adoção pelo cotonicultores de Goiás mostyra qye é uma alternativa de produção viável e sustentável, e tem demostrado que pode vis a subistituir o sistema convencional.
650 $aAdubação
650 $aAlgodão
650 $aPlantio Direto
653 $aAlgodoeiro
653 $aSistema de plantio direto
653 $aSistema plantio direto
700 1 $aCARVALHO, M. da C. S.
700 1 $aFREITAS, P. L. de
700 1 $aOLIVEIRA JUNIOR, J. P.
700 1 $aLEANDRO, W. M.
700 1 $aSILVA, T. M. da
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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