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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/03/2008 |
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Data da última atualização: |
26/02/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
HIGASHI, S.; HUNGRIA, M.; BARCELLOS, F. G. |
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Afiliação: |
Susan Higashi, Embrapa Soja; Mariangela Hungria, Embrapa soja; Fernando Gomes Barcellos, Embrapa Soja. |
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Título: |
Creation of a culture collection database of dizotrophic an plant growth promoter bacteria of Embrapa Soja. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 3., 2007, São Paulo. X-Meeting 2007: proceedings. Campinas: Associação Brasileira de Bioinformática e Biologia Computacional, 2007. |
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Páginas: |
p. 238. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Coleção de cultura de bactérias. |
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Thesagro: |
Base de Dados. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00717naa a2200169 a 4500
001 1456412
005 2013-02-26
008 2007 bl uuuu u00u1 u #d
100 1 $aHIGASHI, S.
245 $aCreation of a culture collection database of dizotrophic an plant growth promoter bacteria of Embrapa Soja.
260 $c2007
300 $ap. 238.
650 $aBase de Dados
653 $aColeção de cultura de bactérias
700 1 $aHUNGRIA, M.
700 1 $aBARCELLOS, F. G.
773 $tIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 3., 2007, São Paulo. X-Meeting 2007: proceedings. Campinas: Associação Brasileira de Bioinformática e Biologia Computacional, 2007.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
09/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; FREITAS, E. P. de; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. |
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Afiliação: |
JESSICA MARIA LEITE DOS SANTOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; JANAÉLIA FERREIRA VASCONCELOS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; GRACIELLE ARAÚJO FROTA, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; EDILSON PEREIRA DE FREITAS, Pós-graduação - INTA / UNINTA - Sobral, CE, Brazil; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; CLAUDIA MARIA LEAL BEVILAQUA, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC. |
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Título: |
Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Experimental Parasitology, v. 205, article 107734, Aug. 2019. |
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DOI: |
http://dx.doi.org/10.1016/j.exppara.2019.107734 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus. MenosAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations bu... Mostrar Tudo |
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Palavras-Chave: |
Anthelmintic resistance; Antinematodal Agents; Drug effects; Haemonchiasis; Nematode parasite; Real-time PCR. |
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Thesaurus NAL: |
Benzimidazoles; Drug resistance; Gene frequency; Genetics; Helminths; Levamisole; Nematode larvae; Parasitology; Pharmacology; Sequence alignment; Sheep diseases; Tetramisole. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02921naa a2200433 a 4500
001 2116311
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.exppara.2019.107734$2DOI
100 1 $aSANTOS, J. M. L. dos
245 $aQuantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus.$h[electronic resource]
260 $c2019
520 $aAbstract: Parasitism by Haemonchus contortus is one of the main limiting factors in small ruminant production around the globe. Although several studies suggest the use of integrated management practices, these parasites have been controlled essentially with synthetic anthelmintic drugs. The resistance mechanism against the imidazothiazole derivative levamisole in Haemonchus contortus has not been fully described. Recently, resistance was associated with a 63bp deletion in the Hco-acr-8b gene that encodes a subunit for a nicotinic acetylcholine receptor. This study aimed to standardize a real time PCR (qPCR) protocol for levamisole resistance diagnosis in H. contortus populations based on this polymorphism and use it to characterize 23 field H. contortus populations obtained from different localities of Ceará State, Northeast Brazil. In addition, two populations of H. contortus were used as a standard of susceptibility and resistance, Inbred Strain Edinburgh (ISE) and Kokstad, respectively. Larval development tests (LDT) were performed on five field isolates and both EC50 and EC95 were estimated. LDT EC95 values provided a wider interval between susceptible and resistant populations than EC50 values (EC95?=?1.96-57.93??M; EC50?=?0.05-0.39??M), and were found to be more appropriate for differentiating them. Real time PCR results showed resistance allele frequencies ranged from 20.9 to 76.7%. Our results suggest that levamisole resistance may be present in field populations but it is not as widespread as benzimidazole resistance. This methodology may be useful to monitor levamisole resistance in field populations of H. contortus.
650 $aBenzimidazoles
650 $aDrug resistance
650 $aGene frequency
650 $aGenetics
650 $aHelminths
650 $aLevamisole
650 $aNematode larvae
650 $aParasitology
650 $aPharmacology
650 $aSequence alignment
650 $aSheep diseases
650 $aTetramisole
653 $aAnthelmintic resistance
653 $aAntinematodal Agents
653 $aDrug effects
653 $aHaemonchiasis
653 $aNematode parasite
653 $aReal-time PCR
700 1 $aVASCONCELOS, J. F.
700 1 $aFROTA, G. A.
700 1 $aFREITAS, E. P. de
700 1 $aTEIXEIRA, M.
700 1 $aVIEIRA, L. da S.
700 1 $aBEVILAQUA, C. M. L.
700 1 $aMONTEIRO, J. P.
773 $tExperimental Parasitology$gv. 205, article 107734, Aug. 2019.
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