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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
26/08/2021 |
Data da última atualização: |
23/02/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, P. A. O.; LIMA, S. M. de F.; MARTINS, D. C. M.; AMORIM, I. A.; LACORTE, C. C.; ALMEIDA, J. A. de; FRANCO, O. L. F.; REZENDE, T. M. B. |
Afiliação: |
POLIANA AMANDA OLIVEIRA SILVA, UNB; STELLA MARIS DE FREITAS LIMA, UCB; DANILO CÉSAR MOTA MARTINS, UNB; INGRID AQUINO AMORIM, UCB; CRISTIANO CASTRO LACORTE, Cenargen; JEESSER ALVES DE ALMEIDA, UFMS; OCTÁVIO LUIZ FRANCO, UCB; TAIA MARIA BERTO REZENDE, UNB. |
Título: |
Concentrated MTA Repair HP reduced biofilm and can cause reparative action at a distance. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
International Endodontic Journal, v. 54, p. 1925-1936, 2021. |
DOI: |
https://doi.org/10.1111/iej.13592 |
Idioma: |
Inglês |
Notas: |
Na publicação: Cristiano Lacorte. |
Palavras-Chave: |
Antibiofilm; Bioactivity; Bioceramic materials; Mineral trioxide aggregate. |
Thesaurus Nal: |
Cytotoxicity. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00883naa a2200277 a 4500 001 2133864 005 2022-02-23 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1111/iej.13592$2DOI 100 1 $aSILVA, P. A. O. 245 $aConcentrated MTA Repair HP reduced biofilm and can cause reparative action at a distance.$h[electronic resource] 260 $c2021 500 $aNa publicação: Cristiano Lacorte. 650 $aCytotoxicity 653 $aAntibiofilm 653 $aBioactivity 653 $aBioceramic materials 653 $aMineral trioxide aggregate 700 1 $aLIMA, S. M. de F. 700 1 $aMARTINS, D. C. M. 700 1 $aAMORIM, I. A. 700 1 $aLACORTE, C. C. 700 1 $aALMEIDA, J. A. de 700 1 $aFRANCO, O. L. F. 700 1 $aREZENDE, T. M. B. 773 $tInternational Endodontic Journal$gv. 54, p. 1925-1936, 2021.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
07/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
Palavras-Chave: |
Cultura do embrião; Sexagem. |
Thesagro: |
Gado. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500 001 1950734 005 2023-03-07 008 2011 bl uuuu u00u1 u #d 100 1 $aMACHADO, G. M. 245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource] 260 $c2011 520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. 650 $aGado 653 $aCultura do embrião 653 $aSexagem 700 1 $aCAIXETA, E. S. 700 1 $aLUCCI, C. M. 700 1 $aRUMPF, R. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tZygote$gv. 20, p. 123-134, 2011.
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