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| 1. |  | PIMENTEL, M. S.; MARTORANO, L. G.; MONTEIRO, D. C.; BATALHA, S. S. A.; BARBOSA, AL. M. da S. Ocorrência de espécies vegetais nativas da Amazônia e suas distribuições em dois municípios no entorno da Floresta Nacional do Tapajós: ênfase na família Arecacea. In: SIMPÓSIO DE ESTUDOS E PESQUISAS EM CIÊNCIAS AMBIENTAIS NA AMAZÔNIA, 2., 2013, Belém, PA. [Anais]. [Belém, PA]: Universidade do Estado do Pará, Centro de Ciências Naturais e Tecnologia, 2013. v. 2, p. 265-270.| Biblioteca(s): Embrapa Amazônia Oriental. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/03/2016 |
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Data da última atualização: |
30/03/2016 |
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Autoria: |
BERNARDO, F. D.; CONHIZAK, C.; AMBROSINI, F.; JESUS, F. P. K. de; SANTURIO, J. M.; KOMMERS, G. D.; ELIAS, F.; FRANCISCATO, C. |
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Afiliação: |
FÁBIO D. BERNARDO, UFFS; CLEVERSON CONHIZAK, Médico veterinário autônomo; FRANCIELLI AMBROSINI, UFFS; FRANCIELLI P. K. de JESUS, UFRGS; JANIO M. SANTURIO, UFSM; GLAUCIA D. KOMMERS, UFSM; FABIANA ELIAS, UFFS; CATARINA FRANCISCATO, UFFS. |
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Título: |
Pythiosis in sheep from Paraná, southern Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 513-517, jun. 2015 |
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Idioma: |
Inglês |
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Conteúdo: |
This paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nasal cavity were positive for Pythium insidiosum by Grocott?s method and immunohistochemistry, the last one considered to be confirmatory for the pathogen diagnostic. This report has an important epidemiological aspect, as it is the first case of pythiosis in sheep confirmed by serology in South Brazil and an alert of possible infection by the pathogen in floodplains. MenosThis paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nas... Mostrar Tudo |
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Palavras-Chave: |
Immunehistochemistry; Imuno-histoquímica. |
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Thesagro: |
ELISA; Histopatologia; Ovino. |
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Thesaurus NAL: |
Enzyme-linked immunosorbent assay; Pythium insidiosum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141834/1/Pythiosis-in-sheep.pdf
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Marc: |
LEADER 02675naa a2200289 a 4500
001 2042199
005 2016-03-30
008 2015 bl uuuu u00u1 u #d
100 1 $aBERNARDO, F. D.
245 $aPythiosis in sheep from Paraná, southern Brazil.
260 $c2015
520 $aThis paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nasal cavity were positive for Pythium insidiosum by Grocott?s method and immunohistochemistry, the last one considered to be confirmatory for the pathogen diagnostic. This report has an important epidemiological aspect, as it is the first case of pythiosis in sheep confirmed by serology in South Brazil and an alert of possible infection by the pathogen in floodplains.
650 $aEnzyme-linked immunosorbent assay
650 $aPythium insidiosum
650 $aELISA
650 $aHistopatologia
650 $aOvino
653 $aImmunehistochemistry
653 $aImuno-histoquímica
700 1 $aCONHIZAK, C.
700 1 $aAMBROSINI, F.
700 1 $aJESUS, F. P. K. de
700 1 $aSANTURIO, J. M.
700 1 $aKOMMERS, G. D.
700 1 $aELIAS, F.
700 1 $aFRANCISCATO, C.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 513-517, jun. 2015
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Embrapa Unidades Centrais (AI-SEDE) |
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