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1. | | PEREIRA, G. S.; ALMEIDA, V. C.; GOMES, M. C. C.; LUCENA, V. S.; OLIVEIRA, T. S.; MENEZES, I. P. P.; HOFFMANN, L. V.; BARROSO, P. A. V. Diversidade genética em algodoeiros não-cultivados do estado de Alagoas usando marcadores microssatélites. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. Biblioteca(s): Embrapa Algodão. |
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Registros recuperados : 1 | |
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Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
22/03/2010 |
Data da última atualização: |
20/11/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
FRANÇA, L. V. de; NASCIMENTO, W. M.; CARMONA, R.; FREITAS, R. A. de. |
Afiliação: |
Leomara Vieira de França, Universidade Federal de Pelotas; WARLEY MARCOS NASCIMENTO, CNPH; Ricardo Carmona, Universidade de Brasília; RAQUEL ALVES DE FREITAS, SIN. |
Título: |
Viability of eggplant pollen. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 9, p. 320-327, 2009. |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to test methodologies to evaluate the pollen viability of eggplant. The pollen germination was tested in vitro, incubating the pollen during 0.5 and 16 hours in different sucrose concentrations (0; 2.5; 5.0; 7.5 and 10.0 g L -1). For the in vivo germination test, the pollen was placed during 4 and 24 hours in the flower stigma and after that stained with aniline blue and evaluated in an ultraviolet light microscope. The viability of the pollen grains was also evaluated in tetrazolium solutions (at the concentrations of 1%; 0.75% and 0.5%) and the staining was evaluated after 24 hours. The highest in vitro pollen germination was 10.8%, showing the necessity for a better calibration of the culture medium. The in vivo pollen germination was 66%, showing that this is an adequate methodology to estimate pollen viability in eggplant. The methodologies involving tetrazolium solution weren’t efficient to estimate pollen viability evaluation, because of the deficient pollen staining. |
Palavras-Chave: |
Cultivo in vitro; Cultivo in vivo; Germinação in vitro; Germinação in vivo; Tetrazólio; Variabilidade polínica em berinjela. |
Thesagro: |
Berinjela; Germinação; Pólen; Semente; Solanum Melongena. |
Thesaurus NAL: |
Pollen germination; Tetrazolium. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/17667/1/franca-viability.pdf
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Marc: |
LEADER 01855naa a2200313 a 4500 001 1780407 005 2018-11-20 008 2009 bl uuuu u00u1 u #d 100 1 $aFRANÇA, L. V. de 245 $aViability of eggplant pollen. 260 $c2009 520 $aThis study aimed to test methodologies to evaluate the pollen viability of eggplant. The pollen germination was tested in vitro, incubating the pollen during 0.5 and 16 hours in different sucrose concentrations (0; 2.5; 5.0; 7.5 and 10.0 g L -1). For the in vivo germination test, the pollen was placed during 4 and 24 hours in the flower stigma and after that stained with aniline blue and evaluated in an ultraviolet light microscope. The viability of the pollen grains was also evaluated in tetrazolium solutions (at the concentrations of 1%; 0.75% and 0.5%) and the staining was evaluated after 24 hours. The highest in vitro pollen germination was 10.8%, showing the necessity for a better calibration of the culture medium. The in vivo pollen germination was 66%, showing that this is an adequate methodology to estimate pollen viability in eggplant. The methodologies involving tetrazolium solution weren’t efficient to estimate pollen viability evaluation, because of the deficient pollen staining. 650 $aPollen germination 650 $aTetrazolium 650 $aBerinjela 650 $aGerminação 650 $aPólen 650 $aSemente 650 $aSolanum Melongena 653 $aCultivo in vitro 653 $aCultivo in vivo 653 $aGerminação in vitro 653 $aGerminação in vivo 653 $aTetrazólio 653 $aVariabilidade polínica em berinjela 700 1 $aNASCIMENTO, W. M. 700 1 $aCARMONA, R. 700 1 $aFREITAS, R. A. de 773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 9, p. 320-327, 2009.
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Embrapa Hortaliças (CNPH) |
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