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| 12. |  | MIRANDA, M. M. P.; FORATO, L. A. Desenvolvimento de sonda de RMN com bobinas acopladas indutivamente. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. p. 24. Editores técnicos: João de Mendonça Naime, Caue Ribeiro, Maria Alice Martins, Elaine Cristina Paris, Paulino Ribeiro Villas Boas, Ladislau Marcelino Rabello. (Embrapa Instrumentação. Documentos, 57).| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 225 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
22/12/1999 |
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Data da última atualização: |
22/12/1999 |
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Autoria: |
BERNARDES FILHO, R.; FORATO, L. A.; COLNAGO, L. A. |
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Título: |
Evaluation of the quantitatives methods to determine the secondary structure of proteins by FTIR. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu, MG. Programa e resumos... [S.l.]: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1996. |
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Páginas: |
p.129. Ref.M-56. |
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Idioma: |
Inglês |
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Conteúdo: |
The quantification of secondary structure from infrared bands is not simple and present serious problems. In this paper, we evaluate the quantitatives aspects of "resolution enhancement" and "pattern recognition" methods. In the first case we studied the Fourier deconvolution technique to enhance the resolution and use a fitting program to resolve the individuals bands. The results show that this method is not adequate to quantify the secondary structures. The major problems are the strong influence of the operator in selecting the parameters to fit the data, the correct assignment of the bands, etc. We have observed up to 50% in error using this method. The pattern recognition technique is based on a partial least squares method. It is necessary to obtain a calibration matrix using the infrared spectra and the proportions of secondary structure from X rays. The user almost does not interfere in the process.The problems are the definition of the types of secondary structures to use (alfa helix, beta sheet, beta turns, etc) and chose the method for calculate them from X rays data bank. We tested Levitt & Greer; Kabsch & Sander, Kabsch & Sander Modified algorithms to calculate secondary structures and the FTIR results show a better correlation when we use alfa helix, beta sheet and undefined structures with Levitt & Greer calculation. |
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Palavras-Chave: |
Estrutura secundaria; FTIR; Protein; Secondary structure. |
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Thesagro: |
Proteína. |
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Categoria do assunto: |
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Marc: |
LEADER 02118naa a2200217 a 4500
001 1026966
005 1999-12-22
008 1996 bl --- 0-- u #d
100 1 $aBERNARDES FILHO, R.
245 $aEvaluation of the quantitatives methods to determine the secondary structure of proteins by FTIR.
260 $c1996
300 $ap.129. Ref.M-56.
520 $aThe quantification of secondary structure from infrared bands is not simple and present serious problems. In this paper, we evaluate the quantitatives aspects of "resolution enhancement" and "pattern recognition" methods. In the first case we studied the Fourier deconvolution technique to enhance the resolution and use a fitting program to resolve the individuals bands. The results show that this method is not adequate to quantify the secondary structures. The major problems are the strong influence of the operator in selecting the parameters to fit the data, the correct assignment of the bands, etc. We have observed up to 50% in error using this method. The pattern recognition technique is based on a partial least squares method. It is necessary to obtain a calibration matrix using the infrared spectra and the proportions of secondary structure from X rays. The user almost does not interfere in the process.The problems are the definition of the types of secondary structures to use (alfa helix, beta sheet, beta turns, etc) and chose the method for calculate them from X rays data bank. We tested Levitt & Greer; Kabsch & Sander, Kabsch & Sander Modified algorithms to calculate secondary structures and the FTIR results show a better correlation when we use alfa helix, beta sheet and undefined structures with Levitt & Greer calculation.
650 $aProteína
653 $aEstrutura secundaria
653 $aFTIR
653 $aProtein
653 $aSecondary structure
700 1 $aFORATO, L. A.
700 1 $aCOLNAGO, L. A.
773 $tIn: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 25., maio 1996, Caxambu, MG. Programa e resumos... [S.l.]: Sociedade Brasileira de Bioquimica e Biologia Molecular, 1996.
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