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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Meio Ambiente. |
Data corrente: |
07/06/2022 |
Data da última atualização: |
08/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
AMARAL, M. do; FREITAS, A. C. O.; SANTOS, A. S.; SANTOS, E. C. dos; FERREIRA, M. M.; GESTEIRA, A. da S.; GRAMACHO, K. P.; MARINHO-PRADO, J. S.; PIROVANI, C. P. |
Afiliação: |
MILENA DO AMARAL, UESC; ANA CAMILA OLIVEIRA FREITAS, UESC; ARIANA SILVA SANTOS, UESC; EVERTON CRUZ DOS SANTOS, Instituto do Câncer José Alencar Gomes da Silva; MONALIZA MACÊDO FERREIRA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; KARINA PERES GRAMACHO, CEPEC; JEANNE SCARDINI MARINHO PRADO, CNPMA; CARLOS PRIMINHO PIROVANI, UESC. |
Título: |
TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Scientific Reports, v. 12, n. 1, article 680, 2022. |
DOI: |
https://doi.org/10.1038/s41598-021-04700-y |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops. MenosAbstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and ot... Mostrar Tudo |
Thesagro: |
Cacau; Inibidor de Enzima; Praga de Planta; Protease; Resistência; Theobroma Cacao. |
Thesaurus Nal: |
Disease resistance; Helicoverpa armigera; Insecticide resistance; Trypsin inhibitors. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02552naa a2200349 a 4500 001 2143860 005 2022-06-08 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-021-04700-y$2DOI 100 1 $aAMARAL, M. do 245 $aTcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.$h[electronic resource] 260 $c2022 520 $aAbstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops. 650 $aDisease resistance 650 $aHelicoverpa armigera 650 $aInsecticide resistance 650 $aTrypsin inhibitors 650 $aCacau 650 $aInibidor de Enzima 650 $aPraga de Planta 650 $aProtease 650 $aResistência 650 $aTheobroma Cacao 700 1 $aFREITAS, A. C. O. 700 1 $aSANTOS, A. S. 700 1 $aSANTOS, E. C. dos 700 1 $aFERREIRA, M. M. 700 1 $aGESTEIRA, A. da S. 700 1 $aGRAMACHO, K. P. 700 1 $aMARINHO-PRADO, J. S. 700 1 $aPIROVANI, C. P. 773 $tScientific Reports$gv. 12, n. 1, article 680, 2022.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
19/05/2000 |
Data da última atualização: |
05/08/2004 |
Autoria: |
MARTINS FILHO, C. A. S.; FONTES, M. P. F.; FONTES, L. E. F.; SFREDO, G. J.; FREIRE, F. M.; FRANCO, M. |
Título: |
Produção de matéria seca e absorção de fósforo por dois cultivares de soja (Glycine max (L.) Merr.) em função de níveis de fósforo de um solo de Minas Gerais. |
Ano de publicação: |
1979 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 17., 1979, Manaus. Resumos... [S. l.]: Sociedade Brasileira de Ciencia do Solo, 1979. |
Páginas: |
p.31. |
Idioma: |
Português |
Conteúdo: |
Em experimento conduzido em casa-de-vegetacao, estudou-se a producao de materia seca e a absorcao de fosforo por dois cultivares de soja ('IAC-2' e 'Vicoja'), em funcao de niveis de fosforo (0 - 100 - 200 e 300 ppm) em um latossolo Vermelho-Amarelo do Estado de Minas Gerais. Ajustaram-se equacoes de segundo grau entre niveis de fosforo e os parametros producao de materia seca do sistema radicular e producao de materia seca da parte aerea. Encontrou-se uma relacao linear entre a adubacao fosfatada e a concentracao de fosforo na parte aerea. Pelas equacoesajustadas calcularam-se as quantidades de fosforo necessarias para as producoes maximas estimadas, sendo tambem obtidos os niveis criticos de fosforo para a parte aerea dos cultivares estudados. |
Palavras-Chave: |
Brasil; Dry matter; Minas Gerais; Soils; Solos; Soybean. |
Thesagro: |
Fósforo; Glycine Max; Matéria Seca; Soja. |
Thesaurus NAL: |
Brazil; phosphorus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01762naa a2200337 a 4500 001 1459491 005 2004-08-05 008 1979 bl uuuu u00u1 u #d 100 1 $aMARTINS FILHO, C. A. S. 245 $aProdução de matéria seca e absorção de fósforo por dois cultivares de soja (Glycine max (L.) Merr.) em função de níveis de fósforo de um solo de Minas Gerais. 260 $c1979 300 $ap.31. 520 $aEm experimento conduzido em casa-de-vegetacao, estudou-se a producao de materia seca e a absorcao de fosforo por dois cultivares de soja ('IAC-2' e 'Vicoja'), em funcao de niveis de fosforo (0 - 100 - 200 e 300 ppm) em um latossolo Vermelho-Amarelo do Estado de Minas Gerais. Ajustaram-se equacoes de segundo grau entre niveis de fosforo e os parametros producao de materia seca do sistema radicular e producao de materia seca da parte aerea. Encontrou-se uma relacao linear entre a adubacao fosfatada e a concentracao de fosforo na parte aerea. Pelas equacoesajustadas calcularam-se as quantidades de fosforo necessarias para as producoes maximas estimadas, sendo tambem obtidos os niveis criticos de fosforo para a parte aerea dos cultivares estudados. 650 $aBrazil 650 $aphosphorus 650 $aFósforo 650 $aGlycine Max 650 $aMatéria Seca 650 $aSoja 653 $aBrasil 653 $aDry matter 653 $aMinas Gerais 653 $aSoils 653 $aSolos 653 $aSoybean 700 1 $aFONTES, M. P. F. 700 1 $aFONTES, L. E. F. 700 1 $aSFREDO, G. J. 700 1 $aFREIRE, F. M. 700 1 $aFRANCO, M. 773 $tIn: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 17., 1979, Manaus. Resumos... [S. l.]: Sociedade Brasileira de Ciencia do Solo, 1979.
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