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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
05/03/2003 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, I. R. P. de; ALVES, V. M. C.; PARENTONI, S. N.; OLIVEIRA, A. C. de; TEIXEIRA, F. F.; ADAM, J. W. Mac. |
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Afiliação: |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; ANTONIO CARLOS DE OLIVEIRA, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS. |
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Título: |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 3, p. 219-224, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The effects of a short-term (80 min) exposure to 222 _uM aluminum (AI) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their leveI of tolerance to AI were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-Iong segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by AI in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to AI, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to AI stress, and that resistance to AI stress is associated with the higher expression of anionic peroxidase isoforms. |
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Palavras-Chave: |
Crescimento radicular; Isoformas de peroxidase; Toxicidade do alumínio. |
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Thesagro: |
Milho. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32872/1/Change-root.pdf
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Marc: |
LEADER 02255naa a2200229 a 4500
001 1480546
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aSOUZA, I. R. P. de
245 $aChange in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines.$h[electronic resource]
260 $c2002
520 $aThe effects of a short-term (80 min) exposure to 222 _uM aluminum (AI) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their leveI of tolerance to AI were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-Iong segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by AI in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to AI, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to AI stress, and that resistance to AI stress is associated with the higher expression of anionic peroxidase isoforms.
650 $aMilho
653 $aCrescimento radicular
653 $aIsoformas de peroxidase
653 $aToxicidade do alumínio
700 1 $aALVES, V. M. C.
700 1 $aPARENTONI, S. N.
700 1 $aOLIVEIRA, A. C. de
700 1 $aTEIXEIRA, F. F.
700 1 $aADAM, J. W. Mac.
773 $tBrazilian Journal of Plant Physiology, Londrina$gv. 14, n. 3, p. 219-224, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
05/11/2007 |
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Data da última atualização: |
31/07/2018 |
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Autoria: |
BRUEL, D. C.; CARPENTIERI-PÍPOLO; GERAGE, A. C.; FONSECA JÚNIOR, N. da S.; PRETE, C. E. C.; RUAS, C. de F.; RUAS, P. M.; SOUZA, S. G. H. de; GARBUGLIO, D. D. |
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Afiliação: |
Daniela Cristina Bruel, Universidade Estadual de Londrina - UEL/Departamento de Agronomia; Valéria Carpentieri-Pípolo, Universidade Estadual de Londrina - UEL/Departamento de Agronomia; Antonio Carlos Gerage, Instituto Agronômico do Paraná - IAPAR/Departamento de Agronomia; Nelson da Silva Fonseca Júnior, Instituto Agronômico do Paraná - IAPAR/Departamento de Agronomia; Cássio Egídio Cavenaghi Prete, Universidade Estadual de Londrina - UEL/Departamento de Agronomia; Claudete de Fátima Ruas, Universidade Estadual de Londrina - UEL/Departamento de Biologia; Paulo Maurício Ruas, Universidade Estadual de Londrina - UEL/Departamento de Biologia; Silvia Graciele Hülse de Souza, Universidade Estadual de Londrina - UEL/Departamento de Agronomia; Deoclécio Domingos Garbuglio, Universidade Estadual de Londrina - UEL/Departamento de Agronomia. |
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Título: |
Genetic distance estimated by RAPD markers and its relationship with hybrid performance in maize. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 10, p. 1491-1498, out. 2006 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Distância genética estimada por marcadores RAPD e sua relação com o desempenho de híbridos em milho. |
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Conteúdo: |
The objective of this work was to evaluate the genetic diversity of 16 maize inbred lines, and to determine the correlation between genetic distance and hybrid performance, using random amplified polymorphic DNA (RAPD) molecular markers. Twenty-two different random primers were used, which resulted in the amplification of 265 fragments, 237 (84.44%) of them being polymorphic. A genetic similarity matrix was created from the RAPD data, using Jaccard coefficient, and a dendrogram was constructed. Hybrid analyses were carried out using random block design and Griffing method VI for diallel crossings. The genetic associations showed five distinct heterotic groups. Correlations between genetic divergences detected by RAPD, as well as the means observed in the diallel crossings were positive and significant for plant height, ear height, prolificacy, and grain weight. The correlation of genetic divergences, detected by RAPD, and the specific combining ability between heterotic group associations, showed significance in all characteristics under study, except prolificacy. A direct relationship between genetic divergence and productivity was found in 79.2% of the 120 hybrids confirming the hypothesis that genetic divergence is directly related to the performance of hybrids and is efficient in predicting it. |
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Palavras-Chave: |
dialelo; diallel; heterotic patterns; marcadores moleculares; molecular markers; padrões heteróticos. |
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Thesagro: |
Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107033/1/Genetic.pdf
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Marc: |
LEADER 02357naa a2200313 a 4500
001 1122666
005 2018-07-31
008 2006 bl uuuu u00u1 u #d
100 1 $aBRUEL, D. C.
245 $aGenetic distance estimated by RAPD markers and its relationship with hybrid performance in maize.
260 $c2006
500 $aTítulo em português: Distância genética estimada por marcadores RAPD e sua relação com o desempenho de híbridos em milho.
520 $aThe objective of this work was to evaluate the genetic diversity of 16 maize inbred lines, and to determine the correlation between genetic distance and hybrid performance, using random amplified polymorphic DNA (RAPD) molecular markers. Twenty-two different random primers were used, which resulted in the amplification of 265 fragments, 237 (84.44%) of them being polymorphic. A genetic similarity matrix was created from the RAPD data, using Jaccard coefficient, and a dendrogram was constructed. Hybrid analyses were carried out using random block design and Griffing method VI for diallel crossings. The genetic associations showed five distinct heterotic groups. Correlations between genetic divergences detected by RAPD, as well as the means observed in the diallel crossings were positive and significant for plant height, ear height, prolificacy, and grain weight. The correlation of genetic divergences, detected by RAPD, and the specific combining ability between heterotic group associations, showed significance in all characteristics under study, except prolificacy. A direct relationship between genetic divergence and productivity was found in 79.2% of the 120 hybrids confirming the hypothesis that genetic divergence is directly related to the performance of hybrids and is efficient in predicting it.
650 $aZea Mays
653 $adialelo
653 $adiallel
653 $aheterotic patterns
653 $amarcadores moleculares
653 $amolecular markers
653 $apadrões heteróticos
700 1 $aCARPENTIERI-PÍPOLO
700 1 $aGERAGE, A. C.
700 1 $aFONSECA JÚNIOR, N. da S.
700 1 $aPRETE, C. E. C.
700 1 $aRUAS, C. de F.
700 1 $aRUAS, P. M.
700 1 $aSOUZA, S. G. H. de
700 1 $aGARBUGLIO, D. D.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 41, n. 10, p. 1491-1498, out. 2006
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Embrapa Unidades Centrais (AI-SEDE) |
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