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Registros recuperados : 538 | |
521. | | PUPIN, M. A. F.; OLIVEIRA, M. E. F.; VERGANI, G. B.; LIMA, M. S. D.; SILVA, K. de M.; MONTEIRO, A. W. U.; RAMOS, A. F.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; BARTLEWSKI, P. M.; FONSECA, J. F. da. Comparison of superovulatory responses to a standardized hormonal superstimulation protocol among three indigenous breeds of sheep in Brazil. Small Ruminant Research, v. 211, 2022. 106703. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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522. | | RODRIGUES, J. N. D.; GUIMARÃES, J. D.; FONSECA, J. F. da; PENITENTE-FILHO, J. M.; RANGEL, P. S. C.; RAMÍREZ LÓPEZ, C. J.; FREITAS, R. S.; CASTILHO, E. F. de; RODRIGUES, L. F. de S.; OLIVEIRA, M. E. F.; GARCIA, A. R. Climatic seasons and time of the day influence thermoregulation and testicular hemodynamics in Santa Inês rams raised under humid tropical conditions. Journal of Thermal Biology, v. 114, article 103546, May, 2023. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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523. | | DIAS, J. H.; PUPIN, M. A.; DUARTE, G. S.; ANDRADE, A. B. P. de; BRAIR, V. L.; PAULA, C. J. C. de; SOUSA, M. A. P. de; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Cervical relaxation protocol without estradiol benzoate is efficient to allow transcervical uterine flushing in Dorper ewes. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online). Biblioteca(s): Embrapa Caprinos e Ovinos. |
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524. | | ALVES, N. G.; ASCARI, I. J.; ALVES, A. C.; LIMA, R. R.; GARCIA, I. F. F.; PÉREZ, J. R. O.; JUNQUEIRA, F. B.; FERNANDES, V. P.; VIEIRA, J. A.; MACIEL, L. F. S.; FONSECA, J. F. da; AZIANI, W. L. B.; CASTRO, T. R.; NEPPE, L. M. Different suckling regimens and return to postpartum cyclic ovarian activity in Santa Inês ewes. Reproduction, Fertility and Development, v. 25, n. 1, p. 157-158. Proceedings of the Annual Conference of the International Embryo Transfer Society, Hannover, Germany, 19-22 January, 2013. Papers and Abstracts for Poster Presentation. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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525. | | DIAS, J. H.; VERGANI; GONÇALVES, J. D.; OLIVEIRA, T. A.; PENITENTE-FILHO, J. M.; PEREIRA, V. S. do A.; ESTEVES, S. N.; GARCIA, A. R.; BATISTA, R. I. T. P.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Different doses of pFSH are effective to promote follicular growth, superovulatory response, and embryo yield in White Dorper ewes. Small Ruminant Research, v. 220, n. 103, mar. 2023, 106914. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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526. | | RODRIGUEZ, M. G. K.; MACIEL, G. S.; RAMIREZ USCATEGUI, R. A.; USCATEGUI, R. A. R.; SANTOS, V. J. C.; NOCITI, R. P.; SILVA, P. del A. da; FELICIANO, M. A. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; OLIVEIRA, M. E. F. Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle-stimulating hormone. Reproduction in Domestic Animals, v. 54, n. 3, p. 456-463, Mar. 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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527. | | TAIRA, A. R.; BATISTA, R. I. T. P.; SANTOS, J. D. R.; PINTO, P. H. N.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; SOUZA-FABJAN, J. M. G.; UNGERFELD, R.; FONSECA, J. F. da; BRANDÃO, F. Z. Progestogen supplementation during superovulation leads to higher embryo viability and TGFB1 gene expression in sheep. Animal Reproduction Science, v. 238, 106938, Mar. 2022. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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528. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; PINTO, P. H. N.; SANTOS, J. D. R.; CAMARGO, L. S. de A.; MENCHACA, A.; FONSECA, J. F. da; BRANDÃO, F. Z. Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domestic Animal Endocrinology, v. 74, e106518, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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529. | | VERGANI, G. B.; ARRAIS, A. M.; MELLO, M. R. B. de; DIAS, J. H.; SOUSA, M. A. P. de; MORAIS, M. C. da C.; FERREIRA, H. C.; BEVILAQUA, J. R.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Follicular population at the beginning of pFSH treatment in Morada Nova ewes submitted to estrous synchronization protocols by short- (6 days), medium- (9 days) or long-term (12 days). Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 533, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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530. | | SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. Veterinary Record, e1064, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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531. | | BALARO, M. F. A.; FERNANDES, D. A. de M.; MOURA, L. F. G. M.; SANTOS, A. da S.; FARIA, N. A. C. de; CAVALCANTI, L. M.; GONÇALVES, L. H. S.; RIBEIRO, A. C. S.; DELGADO, K. F.; FONSECA, J. F. da; BRANDÃO, F. Z. Parâmetros biométricos de maior praticidade na estimação da idade fetal por ultrassonografia em cabras da raça Saanen no terço inicial de gestação. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 149. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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532. | | SOUZA, M. A. P. DE; ESTEVES, S. N.; DAHER, L. C. C.; GALLO, S. B.; PEREIRA, V. S. do A.; FONSECA, J. F. da; SILVA, A. G. M. E; BRANDÃO, F. Z.; OLIVEIRA, M. E. F.; BARRETO, A. DO N.; VERGANI, G. B.; GARCIA, A. R. Overnutrition of ewe in late gestation and the impact on placental efficiency and lamb's performance. Animals, v. 13, n. 103, 2023. 17 p. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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533. | | RIBEIRO, A. C. S.; PINTO, P. H. N.; SANTOS, J. D. R.; RAMALHO, N. P.; LAEBER, C. C. R.; BALARO, M. F. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes. Animal Reproduction Science, v. 64, 107459, May, 2024. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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534. | | MOURA, R. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; MELO, C. H. S.; SANCHEZ, D. J. D.; VIEIRA, M. P.; ALMEIDA, T. M.; SEROVA, I. A.; SEROV, O. L.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FREITAS, V. J. F. Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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535. | | MOURA, R. R.; SOUZA, J. M. G.; MELO, C. H. S.; ALBUQUERQUE, E. S.; ALMEIDA, T. M.; VIEIRA, M. P.; BATISTA, R. I. T. P.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FONSECA, J. F. da; FREITAS, V. J. F. Use of two methods for embryo recovery in an embryo transfer program from a founder transgenic goat for human granulocyte colony-Stimulating Factor (hG-CSF). Transgenic Research, v. 21, n. 4, p. 921, Aug. 2012. Abstracts from the UC Davis Transgenic Animal Research Conference VIII, August, 2011. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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536. | | MOURA, R. R.; SOUZA, J. M. G. de; ALMEIDA, T. M. de; VIEIRA, M. P.; MELO, C. H. S. de; BATISTA, R. I. T. P.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FONSECA, J. F. da; FREITAS, V. J. de F. Uso da colheita transcervical de embriões em cabra transgênica para o fator estimulante de colônia de granulócitos humano (HG-CSF). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal: anais. Cumbuco: SBTE, 2011. p. 422. Resumo R224. 1 CD-ROM. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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537. | | BRAIR, V. L.; MAIA, A. L. R. e S.; CORREIA, L. F. L.; PRELLWITZ, L.; PAULA, C. J. C. de; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de. Vitrification technique improves more alterations on gene expression of in vivo-derived sheep blastocysts than slow freezing. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online). Biblioteca(s): Embrapa Caprinos e Ovinos. |
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538. | | FARIA, N. A. C. de; BALARO, M. F. A.; RIBEIRO, A. C. S.; DELGADO, K. F.; FERNANDES, D. A. de M.; SANTOS, A. da S.; CAVALCANTI, L. M.; GONÇALVES, L. H. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BRANDÃO, F. Z. Uso da eCG ou hCG na indução do estro sincronizado em cabras da raça Saanen na contra-estação reprodutiva. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 21., 2015, Belo Horizonte. Anais... Belo Horizonte: CBRA, 2015. p. 150. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 538 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
29/09/2017 |
Data da última atualização: |
24/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Helena Fabiana Reis de Almeida Saraiva, Universidade Federal Fluminense (UFF) - Niteroi, RJ, Brazil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niteroi, RJ, Brazil; Pedro Henrique Nicolau Pinto, UFF - Niteroi, RJ, Brazil; Lilian Santos Ribeiro, UFF - Niteroi, RJ, Brazil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza-Fabjan, UFF - Niteroi, RJ, Brazil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niteroi, RJ, Brazil. |
Título: |
l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Theriogenology, v. 105, p. 150-157, Jan. 2018. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2017.09.022 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. MenosAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control... Mostrar Tudo |
Palavras-Chave: |
Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival. |
Thesagro: |
Ovino. |
Thesaurus NAL: |
Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03336naa a2200433 a 4500 001 2076472 005 2022-11-24 008 2017 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2017.09.022$2DOI 100 1 $aSARAIVA, H. F. R. de A. 245 $al-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.$h[electronic resource] 260 $c2017 520 $aAbstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies. 650 $aCarnitine 650 $aCell culture 650 $aCryopreservation 650 $aEwes 650 $aGene expression 650 $aIn vitro culture 650 $aoocytes 650 $aSheep 650 $aVitrification 650 $aOvino 653 $aAnimal embryos 653 $aATP cycle 653 $aDrug effects 653 $aMitochondrial genetics 653 $aSanta ines 653 $aSurvival 700 1 $aBATISTA, R. I. T. P. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aPINTO, P. H. N. 700 1 $aRIBEIRO, L. S. 700 1 $aOLIVEIRA, C. S. 700 1 $aSOUZA-FABJAN, J. M. G. de 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tTheriogenology$gv. 105, p. 150-157, Jan. 2018.
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