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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/10/2005 |
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Data da última atualização: |
10/10/2005 |
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Autoria: |
SMIDERLE, O. J.; MOURÃO JUNIOR, M.; GIANLUPPI, D.; CASTRO, C. de. |
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Título: |
Adubação nitrogenada para girassol nos cerrados de Roraima. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 16.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 4., 2005, Londrina. Anais... Londrina: Embrapa Soja, 2005. |
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Páginas: |
p. 32-35. |
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Série: |
(Embrapa Soja. Documentos, 261). |
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Idioma: |
Português |
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Notas: |
Organizado por Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf. |
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Conteúdo: |
O objetivo deste trabalho foi definir o melhor nível de adubação nitrogenada para a cultura do girassol nos cerrados de Roraima. para tanto, foram instalados em 2001, 2002 e 2003 experimentos em quatro épocas de semeadura (29/05; 12/06; 25/06 e 08/07) sendo realizadas adubações de 40; 80 e 120 kg ha-1 de N (uréia como fonte) em duas aplicações, 40% aos 15 dias após a emergência e 60% aos 30 dias, bem como testemunha sem aplicação de N. Na semeadura foram aplicados 120 kg ha-1 de P2O5 e 60 kg ha-1 de K2O, além de 60 kg ha-1 de FTE BR 12. Aos 30 dias após emergência dez-se cobertura com cloreto de potássio (60 kg ha-1 de K2O). O delineamento experimental foi de blocos casualizados com 04 repetições, sendo que as parcelas constituíram-se de 04 fileiras úteis de 06m de comprimento. Foram avaliados os parâmetros de produtividade e teores e rendimentos de óleo dos aquênios obtidos. A produtividade do girassol é influenciada pela adubação nitrogenada e a faixa ótima para cultivo do BRS 191 situa-se entre 80-85 kg ha-1 de N. |
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Thesagro: |
Adubação; Girassol; Produtividade. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01853naa a2200229 a 4500
001 1468425
005 2005-10-10
008 2005 bl uuuu u00u1 u #d
100 1 $aSMIDERLE, O. J.
245 $aAdubação nitrogenada para girassol nos cerrados de Roraima.
260 $c2005
300 $ap. 32-35.
490 $a(Embrapa Soja. Documentos, 261).
500 $aOrganizado por Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.
520 $aO objetivo deste trabalho foi definir o melhor nível de adubação nitrogenada para a cultura do girassol nos cerrados de Roraima. para tanto, foram instalados em 2001, 2002 e 2003 experimentos em quatro épocas de semeadura (29/05; 12/06; 25/06 e 08/07) sendo realizadas adubações de 40; 80 e 120 kg ha-1 de N (uréia como fonte) em duas aplicações, 40% aos 15 dias após a emergência e 60% aos 30 dias, bem como testemunha sem aplicação de N. Na semeadura foram aplicados 120 kg ha-1 de P2O5 e 60 kg ha-1 de K2O, além de 60 kg ha-1 de FTE BR 12. Aos 30 dias após emergência dez-se cobertura com cloreto de potássio (60 kg ha-1 de K2O). O delineamento experimental foi de blocos casualizados com 04 repetições, sendo que as parcelas constituíram-se de 04 fileiras úteis de 06m de comprimento. Foram avaliados os parâmetros de produtividade e teores e rendimentos de óleo dos aquênios obtidos. A produtividade do girassol é influenciada pela adubação nitrogenada e a faixa ótima para cultivo do BRS 191 situa-se entre 80-85 kg ha-1 de N.
650 $aAdubação
650 $aGirassol
650 $aProdutividade
700 1 $aMOURÃO JUNIOR, M.
700 1 $aGIANLUPPI, D.
700 1 $aCASTRO, C. de
773 $tIn: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 16.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 4., 2005, Londrina. Anais... Londrina: Embrapa Soja, 2005.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
23/08/2017 |
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Data da última atualização: |
16/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
NASCIMENTO, P. M. P.; SANTOS, G. B.; PENIDO, A. O.; FONSECA, J. F. da; LEITE, R. C. |
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Afiliação: |
Universidade Federal de Minas Gerais (UFMG) -Belo Horizonte, MG, Brazil; Universidade Federal Fluminense (UFF); UFMG -Belo Horizonte, MG, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; UFMG -Belo Horizonte, MG, Brazil. |
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Título: |
Identification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015. |
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Conteúdo: |
Abstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work. MenosAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthes... Mostrar Tudo |
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Palavras-Chave: |
Animal biotechnology; Artrite encefalite caprina; CAEV; Caprine arthritis encephalit virus; In vivo embryos. |
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Thesagro: |
Biotecnologia; Caprino; Doença animal; Reprodução animal; Transferência de embrião; Transmissão de doença. |
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Thesaurus NAL: |
Animal diseases; Disease transmission; Embryo transfer; Goat diseases; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162905/1/CNPC-2015-Identification-of-arthritis.pdf
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Marc: |
LEADER 03618nam a2200361 a 4500
001 2074275
005 2022-05-16
008 2015 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, P. M. P.
245 $aIdentification of arthritis encephalitis caprine (CAEV) in flushing media and embryos fromnaturally infected herd goats.$h[electronic resource]
260 $aAnimal Reproduction, v. 12, n. 3, p. 703, Jul./Sept. 2015.$c2015
500 $aProceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium, September 11th and 12th, 2015.
520 $aAbstract: The goat production in Minas Gerais state has been growing in recent years, especially with intensive dairy production systems. The artificial reproduction biotechnologies are a powerful mechanism for genetics dissemination. However, the caprine arthritis encephalitis virus (CAEV) is present in most of the dairy goats farms, which represent a risk for disease transmission. The aim of this study is to identify the presence of pro-viral and viral CAEV genome in uterine lavage fluid and embryos samples collected by the non cirurgical transcervical assay. Eight goats were selected with 56.0 ± 13,4kg and a body condition score of 3.5 ± 0.75. The animals were all positive for CAEV in "Western Blotting" diagnosis. Oestrus sycronisation and ovarian superovulation was performed from adapted protocol from Fonseca et al, 2006 (Acta Scientiae Veterinariae, v. 34, sul. 1). After transcervical embryo flushing, embryos that had intact ZP, had their inner cell mass aspirated by a micromanipulator, and the product (zona pellucida and inner cell mass) was stored in RNA later. Centrifugation was performed in uterine liquid washings, until the identification of a pellet (pellet 1) and subsequently viral concentration of uterine flushings (pellet 2) was performed. The material obtained (pellets 1 and pellets 2) was submitted to viral and pro-viral genome extraction with Cador Pathogen® mini kit (Qiagen, Germany) assay. Embryonic samples (zona pellucida and inner cell mass) cDNA synthesis was performed from the viral genome with the use of M MLV enzyme according to protocol. The samples were submitted to nested PCR (Fieni, 2010,). The presence of viral and pro-viral genome in pellet (samples 2), but not in the pellet 1 or embryonic samples (zona pellucida and inner cell mass) was identified. Results shows viral replication could happen in the reproductive system from naturally infected goats, what have never been described before. However, embryos recovery from the flushing media did not show to be permissive to virus. This might indicate that the non cirurgical technique can provide virus dilution, since previous studies in embryos collected surgically revealed the presence of the virus pellet in 1, what did not happen in present work.
650 $aAnimal diseases
650 $aDisease transmission
650 $aEmbryo transfer
650 $aGoat diseases
650 $aGoats
650 $aBiotecnologia
650 $aCaprino
650 $aDoença animal
650 $aReprodução animal
650 $aTransferência de embrião
650 $aTransmissão de doença
653 $aAnimal biotechnology
653 $aArtrite encefalite caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aIn vivo embryos
700 1 $aSANTOS, G. B.
700 1 $aPENIDO, A. O.
700 1 $aFONSECA, J. F. da
700 1 $aLEITE, R. C.
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Embrapa Caprinos e Ovinos (CNPC) |
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