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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2012 |
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Data da última atualização: |
21/10/2015 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; UNESP/JABOTICABAL. |
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Título: |
Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 |
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Páginas: |
p. 119 |
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Idioma: |
Português |
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Conteúdo: |
Enucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable. MenosEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated... Mostrar Tudo |
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Palavras-Chave: |
Demecolcine; Meiotic cell cycle. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 03762nam a2200217 a 4500
001 1917825
005 2015-10-21
008 2012 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation.
260 $aIn: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO$c2012
300 $ap. 119
520 $aEnucleation in traditional nuclear transfer (NT) is an invasive procedure for which alternative protocols have been sought. The present study was designed to explore the time course effects of demecolcine, a microtubule-depolymerizing agent, in bovine-activated oocytes submitted to induced chemical enucleation. For that purpose, after 26 h of in vitro maturation, the oocytes were parthenogenetically activated (5 mMionomycin for 5 min and 10 mgmL 1 cycloheximide for 4 h) and treated with demecolcine (0.05 mgmL 1 for 2 h) 2 h after activation. Three groups were established: control (untreated oocytes), activated (oocytes exposed to activation) and deme (oocytes activated and treated with demecolcine). Then the nuclear and microtubular dynamics of the oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 59, 537?545). In each one of 3 replicates, 15 to 30 oocytes were evaluated per group. Oocytes were classified according to microtubule (MT) patterns as follows: present (evident MT), reduced (MT with reduced density), or absent. The results in percentage were submitted toANOVAand means were compared by Tukey test, with a significance level of 5%. Effects of activation were observed after 2 h, when higher rates of oocytes presenting second polar body (2nd PB) extrusion were observed in the groups activated and deme (49.3% in both groups) compared with control (1.7%). At the end of activation treatment (4 h), the activated group presented 81.8% of oocytes with 2nd PB extrusion, whereas it was observed only in 37.8% of oocytes in the deme group. Effects of demecolcine on the microtubules initiated after only 0.5 h of treatment, when an increase (Po0.05) of oocytes with reduced MT was observed in the deme group (26%; control ? 3%; activated ? 0%). After 6 h of culture in demecolcine-free medium, the deme group displayed ,50% of oocytes with reduced MT (control ? 0%; activated ? 39%) and absence of MT in 23% of oocytes, which was superior to other groups (control ? 0%; activated ? 2%). Therefore, we detected a reduction of MT density after exposition of activated oocytes to demecolcine. However, MT were not completely absent in most of the evaluated oocytes, as previously described for bovine oocytes submitted to chemically assisted enucleation (Saraiva et al. 2009 Cloning Stem Cells 11, 141?152; Meng et al. 2011 Cell. Reprogram., in press). Apparently, there was no immediate repolymerization of MT after culture in demecolcine-free medium and this could result in negative consequences for subsequent embryo development. Moreover, demecolcine impaired the second PB extrusion during the activation process, probably due to inhibition of spindle rotation caused by the MT-disrupting drug. Nonetheless, considering the higher cytoplasmatic volume obtained with chemical enucleation and the lesser extent of injuries suffered by recipient oocytes, further studies focusing on the potential of embryo development and the quality of embryos are advisable.
650 $aoocytes
653 $aDemecolcine
653 $aMeiotic cell cycle
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/04/2010 |
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Data da última atualização: |
22/04/2010 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
LOPES, F. C. F.; RIBEIRO, C. G. S.; RIBEIRO, M. T.; RODRIGUES, N. M.; FILHO, H. G. B.; CASTRO, R. J. C.; BARROS, P. A. V.; GAMA, M. A. S. da. |
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Afiliação: |
FERNANDO CESAR FERRAZ LOPES, CNPGL; C. G. S. RIBEIRO, UFMF; MARLICE TEIXEIRA RIBEIRO, CNPGL; N. M. RODRIGUES, UFMF; H. G. B. FILHO, UFJF; R. J. C. CASTRO, UFJF; P. A. V. BARROS, UFJF; MARCO ANTONIO SUNDFELD DA GAMA, CNPGL. |
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Título: |
Milk fatty acid from dairy cows fed increasing levels of soybean oil in diets based on tropical forage. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON RUMINANT PHYSIOLOGY, 11., 2009, Clermont-Ferrand. Proceedings... Clermont-Ferrand: WAP, 2009. |
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Idioma: |
Inglês |
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Thesagro: |
Forrageira Tropical. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 00702naa a2200205 a 4500
001 1697202
005 2010-04-22
008 2009 bl --- 0-- u #d
100 1 $aLOPES, F. C. F.
245 $aMilk fatty acid from dairy cows fed increasing levels of soybean oil in diets based on tropical forage.
260 $c2009
650 $aForrageira Tropical
700 1 $aRIBEIRO, C. G. S.
700 1 $aRIBEIRO, M. T.
700 1 $aRODRIGUES, N. M.
700 1 $aFILHO, H. G. B.
700 1 $aCASTRO, R. J. C.
700 1 $aBARROS, P. A. V.
700 1 $aGAMA, M. A. S. da
773 $tIn: INTERNATIONAL SYMPOSIUM ON RUMINANT PHYSIOLOGY, 11., 2009, Clermont-Ferrand. Proceedings... Clermont-Ferrand: WAP, 2009.
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