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| Registros recuperados : 8 | |
| 3. |  | SILVEIRA, M. H. L. S.; SIQUEIRA, F. G. de; RAU, M.; SILVA, L. da; MOREIRA, L. R. de S.; FILHO, E. X. F.; ANDREAUS, J. Hydrolysis of sugarcane bagasse with enzyme preparations from Acrophialophora nainiana grown on different carbon sources. Biocatalysis and Biotransformation, v. 32, n. 1, p. 53-63, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | NETO, C. B. S.; BONFIM, C. A.; VALE, H. M. M.; ALCANTARA, L. M.; BRASIL, B. dos S. A. F.; FILHO, E. X. F.; SIQUEIRA, F. G. de. Holocellulase from filamentous fungi grown in culture media containing cassava wastewater. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | REGO, A. P. B.; CONCEIÇÃO, A. A.; TEIXEIRA, F. G.; FILHO, E. X. F.; RODRIGUES, D. de S.; SALUM, T. F. C.; SIQUEIRA, F. G. de. Determinação de atividades enzimáticas para celulases e hemicelulases fungicas sob fermentação estado sólido em biomassa de Jacaratia corumbensis e Manihot esculenta. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 19.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 10., 2013, Foz de Iguaçu, PR. [Anais...]. São Paulo: Associação Brasileira de Engenharia Química, 2013. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010.| Biblioteca(s): Embrapa Soja. |
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| 7. | | MENEZES, B. dos S.; MORAIS, K. dos S.; LEITE, T. C. R.; ALCANTARA, L. M.; FILHO, E. X. F.; BRASIL, B. dos S. A. F.; SIQUEIRA, F. G. de. Production of holocellulases and amylases by filamentous fungi grown on alternative substrates. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, O. B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; REIS, A. M. dos; GATEHOUSE, J. A.; SA, M. F. G. de. A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. BMC Biotechnology, v. 11, n. 14, 2011.| Biblioteca(s): Embrapa Cerrados. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
12/11/2013 |
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Data da última atualização: |
21/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
JARAMILLO, P. M. D.; GOMES, H. A. R.; SIQUEIRA, F. G. de; MELLO, M. H. de; FILHO, E. X. F.; MAGALHÃES, P. O. |
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Afiliação: |
Paula M. Duque Jaramillo, UnB; Helder A. Rocha Gomes, UnB; FELIX GONCALVES DE SIQUEIRA, CNPAE; Mauricio Homem-de-Mello, UnB; Edivaldo Ximenes Ferreira Filho, UnB; Pérola O. Magalhães, UnB. |
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Título: |
Liquid-liquid extraction of pectinase produced by Aspergillus oryzae using aqueous two-phase micellar system. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Separation and Purification Technology, v. 120, p. 452-457, 2013. |
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DOI: |
10.1016/j.seppur.2013.09.020 |
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Idioma: |
Inglês |
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Conteúdo: |
Separation and purification of proteins, organic compounds and other biomolecules are feasible by liquid?liquid extraction based on phase separation in aqueous micellar solution. This work aimed to opti-mize the extraction of the pectinase enzyme produced by Aspergillus oryzae in a passion fruit peel media, using a micellar Triton X-114/sodium phosphate buffer system. Best condition for this extraction was evaluated in a 2 3 experimental factorial design with a centered face and three repetitions at the central point. The variable analyzed was the pectinase activity, and in this design, it varied from 0.233 to 0.601 U mL1. The highest activity was obtained in the top phase at conditions 25 C, 8% Triton X-114 (wt/wt) and 20% concentrated crude extract (wt/wt). The results indicated that this aqueous two-phase micellar system (ATPMS) was able to remove visible pigments and possible pectinase inhibitors present in fermented broth, that partitioned in the bottom phase. Therefore, this system can be exploited as a first step in pectinase purification from a low cost production system with potential industrial application. |
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Palavras-Chave: |
Aqueous two-phase micellar system; Passion fruit peel; Pectinase; Triton X-114. |
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Thesagro: |
Aspergillus Oryzae. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $a10.1016/j.seppur.2013.09.020$2DOI
100 1 $aJARAMILLO, P. M. D.
245 $aLiquid-liquid extraction of pectinase produced by Aspergillus oryzae using aqueous two-phase micellar system.$h[electronic resource]
260 $c2013
520 $aSeparation and purification of proteins, organic compounds and other biomolecules are feasible by liquid?liquid extraction based on phase separation in aqueous micellar solution. This work aimed to opti-mize the extraction of the pectinase enzyme produced by Aspergillus oryzae in a passion fruit peel media, using a micellar Triton X-114/sodium phosphate buffer system. Best condition for this extraction was evaluated in a 2 3 experimental factorial design with a centered face and three repetitions at the central point. The variable analyzed was the pectinase activity, and in this design, it varied from 0.233 to 0.601 U mL1. The highest activity was obtained in the top phase at conditions 25 C, 8% Triton X-114 (wt/wt) and 20% concentrated crude extract (wt/wt). The results indicated that this aqueous two-phase micellar system (ATPMS) was able to remove visible pigments and possible pectinase inhibitors present in fermented broth, that partitioned in the bottom phase. Therefore, this system can be exploited as a first step in pectinase purification from a low cost production system with potential industrial application.
650 $aAspergillus Oryzae
653 $aAqueous two-phase micellar system
653 $aPassion fruit peel
653 $aPectinase
653 $aTriton X-114
700 1 $aGOMES, H. A. R.
700 1 $aSIQUEIRA, F. G. de
700 1 $aMELLO, M. H. de
700 1 $aFILHO, E. X. F.
700 1 $aMAGALHÃES, P. O.
773 $tSeparation and Purification Technology$gv. 120, p. 452-457, 2013.
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