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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
14/03/2011 |
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Data da última atualização: |
14/03/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
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Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
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Título: |
Prospection of tissue specific promoters in coffee. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
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Idioma: |
Inglês |
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Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
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Palavras-Chave: |
Coffee. |
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Categoria do assunto: |
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Marc: |
LEADER 02510nam a2200277 a 4500
001 1880640
005 2011-03-14
008 2008 bl uuuu u00u1 u #d
100 1 $aALMEIDA, J. D. de
245 $aProspection of tissue specific promoters in coffee.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008
520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented.
653 $aCoffee
700 1 $aBARROS, L. M. G.
700 1 $aSANTOS, D. B. M.
700 1 $aCOTTA, M. G.
700 1 $aBARBOSA, E. A.
700 1 $aCAÇÃO, S. B.
700 1 $aEIRA, M. T. S. da
700 1 $aALVES, G. S. C.
700 1 $aVINECKY, F.
700 1 $aPEREIRA, L. F. P.
700 1 $aSILVA, F. R. da
700 1 $aANDRADE, A. C.
700 1 $aMARRACCINI, P.
700 1 $aCARNEIRO, M.
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Registro original: |
Embrapa Café (CNPCa) |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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URL |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/04/2008 |
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Data da última atualização: |
17/04/2008 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
COSTA, A. M.; FALEIRO, F. G.; BELLON, G.; FIGUEIREDO, F. C. G.; FERREIRA, M. E. |
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Afiliação: |
Ana Maria Costa, Embrapa Cerrados; Fábio Gelape Faleiro, Embrapa Cerrados; Graciele Bellon, Embrapa Cerrados; Francisco Carneiro Gomes Figueiredo, Embrapa Cerrados; Márcio Elias Ferreira, Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Diversidade genética de gramíneas com base em marcadores gerados com primers SSR de arroz. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Anais... São Lourenço: Sociedade Brasileira de Melhoramento de Plantas, 2007. |
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Idioma: |
Português |
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Palavras-Chave: |
RAPD. |
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Thesagro: |
Arroz; Gramínea; Marcador Molecular; Oryza Sativa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00694nam a2200205 a 4500
001 1189574
005 2008-04-17
008 2007 bl uuuu u01u1 u #d
100 1 $aCOSTA, A. M.
245 $aDiversidade genética de gramíneas com base em marcadores gerados com primers SSR de arroz.
260 $aIn: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Anais... São Lourenço: Sociedade Brasileira de Melhoramento de Plantas$c2007
650 $aArroz
650 $aGramínea
650 $aMarcador Molecular
650 $aOryza Sativa
653 $aRAPD
700 1 $aFALEIRO, F. G.
700 1 $aBELLON, G.
700 1 $aFIGUEIREDO, F. C. G.
700 1 $aFERREIRA, M. E.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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