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| Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
10/01/2010 |
Data da última atualização: |
22/02/2013 |
Autoria: |
CHUNWONGSE, J.; MARTIN, G. B.; TANKSLEY, S. D. |
Título: |
Pre-germination genotypic screening using PCR amplification of half-seeds. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Theoretical and Applied Genetics, v. 86, p. 694-698, 1993. |
Idioma: |
Inglês |
Conteúdo: |
A simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies. |
Palavras-Chave: |
extração de DNA; PCR; RAPD; RFLP; Seleção assistida por marcadores. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01434naa a2200205 a 4500 001 1840296 005 2013-02-22 008 1993 bl --- 0-- u #d 100 1 $aCHUNWONGSE, J. 245 $aPre-germination genotypic screening using PCR amplification of half-seeds. 260 $c1993 520 $aA simple and rapid PCR-based method has been developed for determining the genotype of seeds before germination. Single half-seeds of rice (Oryza sativa L.) and wheat (Triticum aestivum L. em. Thell.) were preincubated, without grinding, in an aqueous extraction buffer. The resulting supernatants were then used in polymerase chain reaction (PCR) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. PCR products of identical size were amplified using either the half-seed extract or DNA isolated from leaf tissue. The remnant half-seeds can be maintained in ordered arrays using microtiter plates allowing the recovery of selected genotypes. Pre-germination genotypic screening of seed populations as described in this report should be useful for a variety of applications in plant breeding and genetics studies. 653 $aextração de DNA 653 $aPCR 653 $aRAPD 653 $aRFLP 653 $aSeleção assistida por marcadores 700 1 $aMARTIN, G. B. 700 1 $aTANKSLEY, S. D. 773 $tTheoretical and Applied Genetics$gv. 86, p. 694-698, 1993.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
22/02/2023 |
Data da última atualização: |
22/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SANTOS, I. B.; FERREIRA, A. U. de C.; RABELO, M. D.; ANHOLETO, L. A.; SOUSA, G. A.; ALEMÁN GAÍNZA, Y.; FIGUEIREDO, A.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
Afiliação: |
ISABELLA B. SANTOS, Universidade Estadual Paulista; AVELARDO URANO DE CARVALHO FERREIRA, CPPSE; MARCIO DIAS RABELO, CPPSE; LUÍS ADRIANO ANHOLETO; GUSTAVO A. SOUSA, Universidade Estadual Paulista; YOUSMEL A. GAÍNZA, Universidade Estadual Paulista; AMANDA FIGUEIREDO, Universidade Estadual Paulista; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Portable near-infrared spectroscopy: a rapid and accurate blood test for diagnosis of Haemonchus contortus infection and for targeted selective treatment of sheep. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
International Journal for Parasitology, v. 53, n. 2, p. 119-127, feb. 2023. |
DOI: |
https://doi.org/10.1016/j.ijpara.2022.12.004 |
Idioma: |
Inglês |
Conteúdo: |
Haemonchus contortus is the most prevalent and important gastrointestinal nematode (GIN) in small ruminants. Since it reduces the packed cell volume (PCV), causing anemia, early diagnosis can be used for targeted selective treatment (TST) of sheep, reducing antiparasitic drug use and anthelmintic resistance. This study aimed to predict PCV values through near-infrared spectroscopy (NIRS) and to develop a classification and diagnosis model of H. contortus infection using PCV values, eggs per gram of feces (EPG) counts and mean daily weight gain (DWG). A total of 1728 spectra were collected from blood samples of 216 lambs with a portable NIR spectroscope. In parallel, other parameters indicative of infection were measured: PCV by hematocrit, FAffa MAlan CHArt (FAMACHA) scores, EPG and DWG. To evaluate the relationship between NIRS spectra and the evaluated parameters, principal component analysis (PCA) was used for an exploratory analysis, regression by the partial least squares method (PLS) for the prediction of PCV values via NIRS, and PCA linear discriminant analysis (PCA-LDA) as a classification model for diagnosis. The absorption peaks in the NIRS region associated with the excitation of overtones of nitrogen-hydrogen (N-H) functional groups of proteins had a strong impact on the principal components (PCs), indicating that blood proteins, especially hemoglobin, can be estimated by the NIRS technique. The model for predicting PCV by PLS presented a standard error of prediction of 2.53, root-mean-square error of 2.48, and coefficient of determination of 0.84, indicating good correlation between the PCV values predicted by the model and the PCV obtained by hematocrit. The PCA-LDA model presented 93.33% sensitivity and 82.18% accuracy, both higher than those of the FAMACHA method, as was expected for resilient Morada Nova lambs. The multivariate models associated with the NIRS technique reported here can be used in the future as a quick and versatile tool for H. contortus infection diagnosis and TST application in lambs. MenosHaemonchus contortus is the most prevalent and important gastrointestinal nematode (GIN) in small ruminants. Since it reduces the packed cell volume (PCV), causing anemia, early diagnosis can be used for targeted selective treatment (TST) of sheep, reducing antiparasitic drug use and anthelmintic resistance. This study aimed to predict PCV values through near-infrared spectroscopy (NIRS) and to develop a classification and diagnosis model of H. contortus infection using PCV values, eggs per gram of feces (EPG) counts and mean daily weight gain (DWG). A total of 1728 spectra were collected from blood samples of 216 lambs with a portable NIR spectroscope. In parallel, other parameters indicative of infection were measured: PCV by hematocrit, FAffa MAlan CHArt (FAMACHA) scores, EPG and DWG. To evaluate the relationship between NIRS spectra and the evaluated parameters, principal component analysis (PCA) was used for an exploratory analysis, regression by the partial least squares method (PLS) for the prediction of PCV values via NIRS, and PCA linear discriminant analysis (PCA-LDA) as a classification model for diagnosis. The absorption peaks in the NIRS region associated with the excitation of overtones of nitrogen-hydrogen (N-H) functional groups of proteins had a strong impact on the principal components (PCs), indicating that blood proteins, especially hemoglobin, can be estimated by the NIRS technique. The model for predicting PCV by PLS presented a standard error of predic... Mostrar Tudo |
Palavras-Chave: |
Diagnosis; FAMACHA method; Linear Discriminate Analysis; NIRS; Parasite control. |
Thesagro: |
Anemia. |
Thesaurus NAL: |
Small ruminants. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03080naa a2200313 a 4500 001 2151857 005 2023-02-22 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ijpara.2022.12.004$2DOI 100 1 $aSANTOS, I. B. 245 $aPortable near-infrared spectroscopy$ba rapid and accurate blood test for diagnosis of Haemonchus contortus infection and for targeted selective treatment of sheep.$h[electronic resource] 260 $c2023 520 $aHaemonchus contortus is the most prevalent and important gastrointestinal nematode (GIN) in small ruminants. Since it reduces the packed cell volume (PCV), causing anemia, early diagnosis can be used for targeted selective treatment (TST) of sheep, reducing antiparasitic drug use and anthelmintic resistance. This study aimed to predict PCV values through near-infrared spectroscopy (NIRS) and to develop a classification and diagnosis model of H. contortus infection using PCV values, eggs per gram of feces (EPG) counts and mean daily weight gain (DWG). A total of 1728 spectra were collected from blood samples of 216 lambs with a portable NIR spectroscope. In parallel, other parameters indicative of infection were measured: PCV by hematocrit, FAffa MAlan CHArt (FAMACHA) scores, EPG and DWG. To evaluate the relationship between NIRS spectra and the evaluated parameters, principal component analysis (PCA) was used for an exploratory analysis, regression by the partial least squares method (PLS) for the prediction of PCV values via NIRS, and PCA linear discriminant analysis (PCA-LDA) as a classification model for diagnosis. The absorption peaks in the NIRS region associated with the excitation of overtones of nitrogen-hydrogen (N-H) functional groups of proteins had a strong impact on the principal components (PCs), indicating that blood proteins, especially hemoglobin, can be estimated by the NIRS technique. The model for predicting PCV by PLS presented a standard error of prediction of 2.53, root-mean-square error of 2.48, and coefficient of determination of 0.84, indicating good correlation between the PCV values predicted by the model and the PCV obtained by hematocrit. The PCA-LDA model presented 93.33% sensitivity and 82.18% accuracy, both higher than those of the FAMACHA method, as was expected for resilient Morada Nova lambs. The multivariate models associated with the NIRS technique reported here can be used in the future as a quick and versatile tool for H. contortus infection diagnosis and TST application in lambs. 650 $aSmall ruminants 650 $aAnemia 653 $aDiagnosis 653 $aFAMACHA method 653 $aLinear Discriminate Analysis 653 $aNIRS 653 $aParasite control 700 1 $aFERREIRA, A. U. de C. 700 1 $aRABELO, M. D. 700 1 $aANHOLETO, L. A. 700 1 $aSOUSA, G. A. 700 1 $aALEMÁN GAÍNZA, Y. 700 1 $aFIGUEIREDO, A. 700 1 $aESTEVES, S. N. 700 1 $aCHAGAS, A. C. de S. 773 $tInternational Journal for Parasitology$gv. 53, n. 2, p. 119-127, feb. 2023.
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