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| Registros recuperados : 3 | |
| 1. |  | VIREQUE, A. A.; CAMARGO, L. S. A.; FERREIRA, E. M.; FERRIANI, R. A.; NAVARRO, P. A. A. S.; ROSA E SILVA, A. A. M. Expression of Hsp-70 and ban genes in blastocysts from bovine oocytes matures in chemically defined medium. In: TALLER GENERAL, 8., 2007, Mar del Plata. Anais... Mar del Plata: Red Latinoamericana de Reproducción Asistida, 2007. p. 17-18.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | VIREQUE, A. A.; CAMARGO, L. S. de A.; FERREIRA, E. M.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; BOS-MIKICH, A.; MARTINS, W. P.; CAMPOS, J. R.; NAVARRO, P. A. A. S.; FERRIANI, R. A. Embryonic development and steroidogenesis of bovine cumulus-oocyte complexes matured in a-mem medium supplemented with pva or pvp-40. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. Theriogenology, v. 71, n. 4, p. 620-627, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 3 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/04/2010 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. |
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Afiliação: |
A. A. VIREQUE, USP; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFRRJ; A. A. M. ROSA E SILVA, Universidade de Brasília; Y. G. WATANABE, Vitrogen; E. M. FERREIRA, USP; P. A. A. S. NAVARRO, USP; W. P. MARTINS, USP; R. A. FERRIANI, USP. |
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Título: |
Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Theriogenology, v. 71, n. 4, p. 620-627, 2009. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2008.09.028 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. MenosIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in ... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; In vitro maturation. |
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Thesaurus NAL: |
embryogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711876/1/Preimplantation-development-and-expression-of-Hsp-70-and-Bax-genes-in-bovine-blastocysts.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.theriogenology.2008.09.028$2DOI
100 1 $aVIREQUE, A. A.
245 $aPreimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules.
260 $c2009
520 $aIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis.
650 $aembryogenesis
653 $aBovine oocytes
653 $aIn vitro maturation
700 1 $aCAMARGO, L. S. de A.
700 1 $aSERAPIÃO, R. V.
700 1 $aROSA E SILVA, A. A. M.
700 1 $aWATANABE, Y. F.
700 1 $aFERREIRA, E. M.
700 1 $aNAVARRO, P. A. A. S.
700 1 $aMARTINS, W. P.
700 1 $aFERRIANI, R. A.
773 $tTheriogenology$gv. 71, n. 4, p. 620-627, 2009.
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Embrapa Gado de Leite (CNPGL) |
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