Data/hora: 02/06/2024 02:24:44

Biblioteca(s): 	Embrapa Unidades Centrais.	
Data corrente: 	15/08/2016	
Data da última atualização: 	15/08/2016	
Autoria: 	SPANAMBERG, A.; FERREIRO, L.; MACHADO, G.; FRAGA, C. F.; ARAUJO, R.	
Afiliação: 	ANDRÉIA SPANAMBERG, PPGCV/UFRGS; LAERTE FERREIRO, UFRGS; GUSTAVO MACHADO, PPGCV/UFRGS; CIBELE FLORIANO FRAGA, PPGCV/UFRGS; RICARDO ARAUJO, IPATIMUP/Universidade do Porto.	
Título: 	Identification and characterization of Aspergillus fumigatus isolates from broilers.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 591-594, jul. 2016.	
Idioma: 	Inglês	
Conteúdo: 	Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung?s samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung?s samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of ?-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung?s samples of broilers.	
Palavras-Chave: 	Genotipagem; Identificação molecular; Molecular identification; Multiplex PCR; PCR multiplex.	
Thesagro: 	Aspergillus Fumigatus; Frango de corte.	
Thesaurus Nal: 	Broiler chickens; Genotyping.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/146359/1/Identification-and-characterization.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
Exemplares: 	AI-SEDE - (59871-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS