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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/06/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. |
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Afiliação: |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
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Título: |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. |
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DOI: |
10.1021/acs.estlett.7b00456 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. MenosABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites ... Mostrar Tudo |
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Palavras-Chave: |
Biodegradação de dioxano; Microbactéria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2082755
005 2019-06-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.estlett.7b00456$2DOI
100 1 $aHE, Y.
245 $aDioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.$h[electronic resource]
260 $c2017
520 $aABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
653 $aBiodegradação de dioxano
653 $aMicrobactéria
700 1 $aMATHIEU, J.
700 1 $aYANG, Y.
700 1 $aYU, P.
700 1 $aSILVA, M. L. B. da
700 1 $aALVAREZ, P. J. J.
773 $tEnvironmental Science & Technology$gv. 4, n. 11, p. 494-499, 2017.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
14/01/2009 |
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Data da última atualização: |
18/01/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
FERREIRA, R. A. D. C.; RAMALHO, M. A. P.; ABREU, A. de F. B. |
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Afiliação: |
Ricardo Augusto Diniz Cabral Ferreira, bolsista Fapemig; Magno Antonio Patto Ramalho, Universidade Federal de Lavras; ANGELA DE FATIMA BARBOSA ABREU, CNPAF. |
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Título: |
Danos causados por Pseudocercospora griseola (Phaeoisariopsis griseola) Sacc. em linhagens de feijoeiro diferindo no grau de resistência. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. |
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Descrição Física: |
1 CD-ROM. |
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Série: |
(IAC. Documentos, 85). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo do presente trabalho foi quantificar o dano do patógeno P. griseola nas condições ambientais do sul de Minas Gerais em linhagens diferindo no grau de resistência. |
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Palavras-Chave: |
CONAFE; Minas Gerais. |
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Thesagro: |
Feijão; Mancha Angular; Phaseolus Vulgaris. |
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Thesaurus NAL: |
Phaeoisariopsis griseola. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/74729/1/207.pdf
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Marc: |
LEADER 01001nam a2200229 a 4500
001 1204786
005 2013-01-18
008 2008 bl uuuu u00u1 u #d
100 1 $aFERREIRA, R. A. D. C.
245 $aDanos causados por Pseudocercospora griseola (Phaeoisariopsis griseola) Sacc. em linhagens de feijoeiro diferindo no grau de resistência.
260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico$c2008
300 $c1 CD-ROM.
490 $a(IAC. Documentos, 85).
520 $aO objetivo do presente trabalho foi quantificar o dano do patógeno P. griseola nas condições ambientais do sul de Minas Gerais em linhagens diferindo no grau de resistência.
650 $aPhaeoisariopsis griseola
650 $aFeijão
650 $aMancha Angular
650 $aPhaseolus Vulgaris
653 $aCONAFE
653 $aMinas Gerais
700 1 $aRAMALHO, M. A. P.
700 1 $aABREU, A. de F. B.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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