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 | Acesso ao texto completo restrito à biblioteca da Embrapa Uva e Vinho. Para informações adicionais entre em contato com cnpuv.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
17/11/2014 |
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Data da última atualização: |
15/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
REVERS, L. F.; WELTER, L. J.; IRALA, P. B.; SILVA, D. C. da; LAMPE, V. S.; OLIVEIRA, P. R. D. de; GARRIDO, L. da R. |
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Afiliação: |
LUIS FERNANDO REVERS, CNPUV; PAULO RICARDO DIAS DE OLIVEIRA, CNPUV; LUCAS DA RESSURREICAO GARRIDO, CNPUV. |
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Título: |
Co-localization of QTLs for seedlessness and downy mildew resistance in grapevine. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Acta Horticulturae, n. 1046, 2014. |
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Páginas: |
p. 449-456 |
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Idioma: |
Inglês |
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Notas: |
Anais do evento "X International Conference on Grapevine Breeding and Genetics". |
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Conteúdo: |
A genetic linkage map was constructed using a pseudo-testcross strategy based on a cross between the seedless Vitis vinifera ?Crimson Seedless? and the complex hybrid ?Villard Blanc?, resistant to downy mildew. A total of 315 DNA markers, including 262 AFLP, 48 simple sequence repeats (SSR), 2 SCARs (sequence characterized amplified region) and 3 minisatellite markers were used to generate a map for each parent. For both parents, 19 linkage groups were obtained, covering 1,111.0 cM and 926.0 cM for ?Villard Blanc? and ?Crimson Seedless?, respectively. The position of SSR loci in the obtained maps is consistent with the genomic sequence. Quantitative Trait Loci (QTLs) for seedlessness and resistance to downy mildew were investigated. Two major effect QTLs for downy mildew resistance and seedlessness were mapped on the same region of linkage group 18. These QTLs explain 25.0-55.7% and 54.0-62.,4% of the total variance, respectively. The MIKCC-Type MADS box gene VvAG3, whose orthologue is involved in Arabidopsis carpel and ovule development, and located in the confidence interval of the seedlessness QTL detected on the LG 18, could be considered as a candidate gene to control seed development in grape. Colocalizations were found in the same region, between the position of the Rpv3 locus, which is very rich in TIR-NBS-LRR genes, and the main QTL identified for downy mildew resistance. Our results demonstrate that the same region of LG 18 contains important genetic determinants for seedlessness and downy mildew resistance in grapevine. Moreover, assessing the allelic variation at these agronomically important loci provides a basis for the development of marker-assisted selection for seedlessness and downy mildew simultaneously. MenosA genetic linkage map was constructed using a pseudo-testcross strategy based on a cross between the seedless Vitis vinifera ?Crimson Seedless? and the complex hybrid ?Villard Blanc?, resistant to downy mildew. A total of 315 DNA markers, including 262 AFLP, 48 simple sequence repeats (SSR), 2 SCARs (sequence characterized amplified region) and 3 minisatellite markers were used to generate a map for each parent. For both parents, 19 linkage groups were obtained, covering 1,111.0 cM and 926.0 cM for ?Villard Blanc? and ?Crimson Seedless?, respectively. The position of SSR loci in the obtained maps is consistent with the genomic sequence. Quantitative Trait Loci (QTLs) for seedlessness and resistance to downy mildew were investigated. Two major effect QTLs for downy mildew resistance and seedlessness were mapped on the same region of linkage group 18. These QTLs explain 25.0-55.7% and 54.0-62.,4% of the total variance, respectively. The MIKCC-Type MADS box gene VvAG3, whose orthologue is involved in Arabidopsis carpel and ovule development, and located in the confidence interval of the seedlessness QTL detected on the LG 18, could be considered as a candidate gene to control seed development in grape. Colocalizations were found in the same region, between the position of the Rpv3 locus, which is very rich in TIR-NBS-LRR genes, and the main QTL identified for downy mildew resistance. Our results demonstrate that the same region of LG 18 contains important genetic determinants f... Mostrar Tudo |
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Palavras-Chave: |
Crimson; Uva sem semente. |
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Thesagro: |
Controle integrado; Genética; Marcador genético; Míldio; Uva; Viticultura; Vitis vinifera. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02686naa a2200325 a 4500
001 2000385
005 2018-10-15
008 2014 bl uuuu u00u1 u #d
100 1 $aREVERS, L. F.
245 $aCo-localization of QTLs for seedlessness and downy mildew resistance in grapevine.$h[electronic resource]
260 $c2014
300 $ap. 449-456
500 $aAnais do evento "X International Conference on Grapevine Breeding and Genetics".
520 $aA genetic linkage map was constructed using a pseudo-testcross strategy based on a cross between the seedless Vitis vinifera ?Crimson Seedless? and the complex hybrid ?Villard Blanc?, resistant to downy mildew. A total of 315 DNA markers, including 262 AFLP, 48 simple sequence repeats (SSR), 2 SCARs (sequence characterized amplified region) and 3 minisatellite markers were used to generate a map for each parent. For both parents, 19 linkage groups were obtained, covering 1,111.0 cM and 926.0 cM for ?Villard Blanc? and ?Crimson Seedless?, respectively. The position of SSR loci in the obtained maps is consistent with the genomic sequence. Quantitative Trait Loci (QTLs) for seedlessness and resistance to downy mildew were investigated. Two major effect QTLs for downy mildew resistance and seedlessness were mapped on the same region of linkage group 18. These QTLs explain 25.0-55.7% and 54.0-62.,4% of the total variance, respectively. The MIKCC-Type MADS box gene VvAG3, whose orthologue is involved in Arabidopsis carpel and ovule development, and located in the confidence interval of the seedlessness QTL detected on the LG 18, could be considered as a candidate gene to control seed development in grape. Colocalizations were found in the same region, between the position of the Rpv3 locus, which is very rich in TIR-NBS-LRR genes, and the main QTL identified for downy mildew resistance. Our results demonstrate that the same region of LG 18 contains important genetic determinants for seedlessness and downy mildew resistance in grapevine. Moreover, assessing the allelic variation at these agronomically important loci provides a basis for the development of marker-assisted selection for seedlessness and downy mildew simultaneously.
650 $aControle integrado
650 $aGenética
650 $aMarcador genético
650 $aMíldio
650 $aUva
650 $aViticultura
650 $aVitis vinifera
653 $aCrimson
653 $aUva sem semente
700 1 $aWELTER, L. J.
700 1 $aIRALA, P. B.
700 1 $aSILVA, D. C. da
700 1 $aLAMPE, V. S.
700 1 $aOLIVEIRA, P. R. D. de
700 1 $aGARRIDO, L. da R.
773 $tActa Horticulturae$gn. 1046, 2014.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
14/02/2012 |
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Data da última atualização: |
17/04/2018 |
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Autoria: |
LIMA, J. A. A.; SILVA, A. K. F. da; ARAGÃO, M. do L.; FERREIRA, N. R. de A.; TEÓFILO, E. M. |
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Afiliação: |
José Albersio Araujo Lima, Universidade Federal do Ceará (UFC); Ana Kelly Firmino da Silva, Universidade Federal do Ceará (UFC); Maria do Livramento Aragão, Universidade Federal do Ceará (UFC); Nádia Rutielly de Araújo Ferreira, Universidade Federal do Ceará (UFC); Elizita Maria Teófilo, UFC, Departamento de Fitotecnia. |
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Título: |
Simple and multiple resistances to viruses in cowpea genotypes. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 46, n. 11, p. 1432-1438, nov. 2011 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Resistência simples e múltipla a vírus em genótipos de caupi. |
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Conteúdo: |
The objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly susceptible, plants with severe mosaic, other systemic symptoms, including systemic necrosis, and positive serology. Simple and multiple resistances to viruses were identified among the evaluated genotypes, but none of them showed multiple immunities to all isolates. Four genotypes showed immunity to all CPSMV isolates, two were immune to CABMV and two showed immunity to CMV. Eleven genotypes showed multiple resistances to two viruses, allowing for the development of new cultivars with more stable and broader resistance. Genotypes Purple Knuckle Hull-55, MNC-03-731C-21 and CNCx284-66E show resistance to CABMV, even when inoculated with CMV. |
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Palavras-Chave: |
Virus control. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus NAL: |
Cowpea aphid-borne mosaic virus; Cowpea severe mosaic virus; Cucumber mosaic virus; serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54098/1/46n11a03.pdf
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Marc: |
LEADER 02249naa a2200253 a 4500
001 1915471
005 2018-04-17
008 2011 bl uuuu u00u1 u #d
100 1 $aLIMA, J. A. A.
245 $aSimple and multiple resistances to viruses in cowpea genotypes.
260 $c2011
500 $aTítulo em português: Resistência simples e múltipla a vírus em genótipos de caupi.
520 $aThe objective of this work was to identify new sources of simple and multiple resistances to Cowpea severe mosaic virus (CPSMV), Cowpea aphid-borne mosaic virus (CABMV) and Cucumber mosaic virus (CMV) isolates in cowpea (Vigna unguiculata). Thirty-three genotypes from the germplasm bank of Universidade Federal do Ceará were tested as to their resistance to four CPSMV isolates, two CABMV isolates and one CMV isolate. Twenty-five days after the first virus inoculations, all inoculated plants, including the asymptomatic ones, were tested by serology. Genotypes were classified as: immune, plants without symptoms and negative serology; resistant, plants with mild mosaic and positive serology; susceptible, plants with mosaic and positive serology; and highly susceptible, plants with severe mosaic, other systemic symptoms, including systemic necrosis, and positive serology. Simple and multiple resistances to viruses were identified among the evaluated genotypes, but none of them showed multiple immunities to all isolates. Four genotypes showed immunity to all CPSMV isolates, two were immune to CABMV and two showed immunity to CMV. Eleven genotypes showed multiple resistances to two viruses, allowing for the development of new cultivars with more stable and broader resistance. Genotypes Purple Knuckle Hull-55, MNC-03-731C-21 and CNCx284-66E show resistance to CABMV, even when inoculated with CMV.
650 $aCowpea aphid-borne mosaic virus
650 $aCowpea severe mosaic virus
650 $aCucumber mosaic virus
650 $aserology
650 $aVigna Unguiculata
653 $aVirus control
700 1 $aSILVA, A. K. F. da
700 1 $aARAGÃO, M. do L.
700 1 $aFERREIRA, N. R. de A.
700 1 $aTEÓFILO, E. M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 46, n. 11, p. 1432-1438, nov. 2011
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Embrapa Unidades Centrais (AI-SEDE) |
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