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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sul. |
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Data corrente: |
13/08/2020 |
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Data da última atualização: |
13/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VAL, A. D. B. do; BREVES, S. de S.; CANÇADO, G. M. de A.; FERREIRA, J. L.; PASQUAL, M. |
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Afiliação: |
AURINETE DAIENN BORGES DO VAL, UESPI; SÂMERA DE SOUZA BREVES, Bolsista EPAMIG; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; JULIANO LINO FERREIRA, CPPSUL; MOACIR PASQUAL, Ufla. |
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Título: |
Use of molecular markers SSR and SCAR for identification of olive accessions. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Bioscience Journal, v. 36, n. 4, p. 1137-1145, July/Aug. 2020. |
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DOI: |
http://dx.doi.org/10.14393/BJ-v36n4a2020-47959 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6. The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively. The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species. MenosABSTRACT: Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6. The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively. The results supported the hypothesis that all accessions belong to th... Mostrar Tudo |
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Palavras-Chave: |
Arbequina; Fingerprinting DNA; Homonimia; Homonymy; Marcadores moleculares; Molecular markers; Oliveira; Sinonimia; Synonymy. |
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Thesagro: |
Olea Europaea. |
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Thesaurus Nal: |
DNA fingerprinting. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215303/1/AP-Use-molecular-2020.pdf
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Marc: |
LEADER 02512naa a2200313 a 4500
001 2124346
005 2020-08-13
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.14393/BJ-v36n4a2020-47959$2DOI
100 1 $aVAL, A. D. B. do
245 $aUse of molecular markers SSR and SCAR for identification of olive accessions.$h[electronic resource]
260 $c2020
520 $aABSTRACT: Morphological and agronomical describers are traditionally used in plant characterization. However, the usage of these describers have some limitations such as susceptibility to abiotic and biotic stress and environmental factors. Furthermore, the describers are not stable over time and many can only be evaluated during the adult phase of the plants, which requires time and physical space. Molecular markers offer numerous advantages compared to the conventional alternatives based on phenotype: they are stable and detectable in all vegetable tissues, and are independent of the environment and development phase. One of the main advantages of the use of molecular markers is the time reduction in the identification of genetic diversity among the studied subjects, as the genotypes may even be described for the seed or seedling phase. Many countries have already adopted molecular markers to identify olive cultivars more accurately. The aim of this study was to evaluate the genetic identity of eight olive accessions supposedly belonging to cultivar Arbequina by using microsatellite (SSR) and Sequence Characterized Amplified Region (SCAR) markers. One accession corresponding to the cultivar was also incorporated into the analysis as a reference genotype. The molecular marker data were analyzed on the software GENALEX6. The markers generated an accumulated PI and PE of 1.26 x 10-6 and 0.949, respectively. The results supported the hypothesis that all accessions belong to the cultivar Arbequina, and the markers can therefore be applied to other varieties of olive species.
650 $aDNA fingerprinting
650 $aOlea Europaea
653 $aArbequina
653 $aFingerprinting DNA
653 $aHomonimia
653 $aHomonymy
653 $aMarcadores moleculares
653 $aMolecular markers
653 $aOliveira
653 $aSinonimia
653 $aSynonymy
700 1 $aBREVES, S. de S.
700 1 $aCANÇADO, G. M. de A.
700 1 $aFERREIRA, J. L.
700 1 $aPASQUAL, M.
773 $tBioscience Journal$gv. 36, n. 4, p. 1137-1145, July/Aug. 2020.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
23/03/2004 |
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Data da última atualização: |
10/11/2021 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
FERREIRA, M. das G. R.; CARVALHO, C. H. S. de; CARNEIRO, A. A.; CARDENAS, F. H. N. |
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Título: |
Introdução de genes em segmentos foliares de cucuaçu (Theobroma grandiflorum schumm.) usando biobalística. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 34, n. 1, p. 279-280, jan./fev. 2004. |
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Idioma: |
Português |
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Conteúdo: |
A técnica da biobalística foi utilizada com o objetivo de adaptar um protocolo para transferência de plantas de cupuaçu. Segmentos foliares de cupuaçu foram bombardeados com um plasmídio contendo o gene reportador da antocianina, utilizando-se as pressões de hélio de 650, 1000 e 1100psi. Após o bombardeamento, os explantes foram transferidos para meio MS por 24 horas e, após este período de incubação, pontos vermelhos foram detectados utilizando-se um estereomicroscópio Stemi SV11 Seiss (Germany). A espressão do gene da antocianina foi observada nas pressões de 650 e 1000psi, entretando necroses foram encontradas nas pressões de 1100psi. Demonstrou-se que os genes C1 e R` da síntese de antocianina, sob o controle do promotor 35S, podem ser utilizados como repórteres para o monitoramento dos eventos de tranformação em cupuaçu. |
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Palavras-Chave: |
Biobalística; Transformação. |
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Thesagro: |
Cupuaçu. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227611/1/cpafro-7734.pdf
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Marc: |
LEADER 01486naa a2200193 a 4500
001 1704858
005 2021-11-10
008 2004 bl uuuu u00u1 u #d
100 1 $aFERREIRA, M. das G. R.
245 $aIntrodução de genes em segmentos foliares de cucuaçu (Theobroma grandiflorum schumm.) usando biobalística.$h[electronic resource]
260 $c2004
520 $aA técnica da biobalística foi utilizada com o objetivo de adaptar um protocolo para transferência de plantas de cupuaçu. Segmentos foliares de cupuaçu foram bombardeados com um plasmídio contendo o gene reportador da antocianina, utilizando-se as pressões de hélio de 650, 1000 e 1100psi. Após o bombardeamento, os explantes foram transferidos para meio MS por 24 horas e, após este período de incubação, pontos vermelhos foram detectados utilizando-se um estereomicroscópio Stemi SV11 Seiss (Germany). A espressão do gene da antocianina foi observada nas pressões de 650 e 1000psi, entretando necroses foram encontradas nas pressões de 1100psi. Demonstrou-se que os genes C1 e R` da síntese de antocianina, sob o controle do promotor 35S, podem ser utilizados como repórteres para o monitoramento dos eventos de tranformação em cupuaçu.
650 $aCupuaçu
653 $aBiobalística
653 $aTransformação
700 1 $aCARVALHO, C. H. S. de
700 1 $aCARNEIRO, A. A.
700 1 $aCARDENAS, F. H. N.
773 $tCiência Rural, Santa Maria$gv. 34, n. 1, p. 279-280, jan./fev. 2004.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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