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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
28/04/1995 |
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Data da última atualização: |
04/04/2017 |
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Autoria: |
CORBEIL, L. B.; HODGSON, J. L.; WIDDERS, P. R. |
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Título: |
Immunoglobulin binding by Tritrichomonas foetus. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v.29, n.12, p.2710-2714, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
A better method for diagnosis of bovine trichomoniasis is needed because culture is slow and somewhat lacking in sensitivity. Immunodiagnosis of Tritrichomonas foetus infection usually involves detection of antigen-antibody reactions with an anti-immunoglobulin conjugate. However, nonspecific immunoglobulin (Ig) bound to the surface of T. foetus, would also be tetected by anti-Ig conjugate and thus would interfere with the specificity of the immunoassay. The goals of this study were to define the binding of bovine immunoglobulins to T. foetus. To determine whether nonimmune binding of Ig to T. foetus occurs, we immunized rabbits with organisms that had been grown in medium containing normal bovine serum and vigorously washed three times with phosphate-buffered saline. The rabbit antiserum had similar titers to T. foetus and to normal bovine serum by enzyme-linked immunosorbent assay (ELISA). Furthermore, two bovine serum proteins were immunoprecipitated by the rabbit antiserum in an immunoelectrophoretogram. One of the serum proteins had a distribution characteristics of IgG2. The rabbit antiserum was then shown to react with purified bovine IgG and IgM by ELISA. Reactivity to IgG was greater. To identify the IgG subisotypes bound and to confirm nonimmune binding of Ig, we grew T. foetus in agammaglobulinemic fetal calf serum and reacted it with IgG1, IgG2 and IgM specific for dinitrophenol (DNP) in ELISA. The binding of IgG2 was greastest that of IgG1 was next, and that of IgM was least. Little competitive inhibition by DNP was detected, indicating that binding of DNP-specific antibodies was predominantly nonimmune rather than antigen-specific Ig binding. We also demosntrated that T. foetus grew well in medium containing agammaglobulinemic fetal calf serum or serum made agammaglobulinemic by ammonium sulfate precipitations of Igs. This may overcome the problem of low specificity in diagnostic assays as a resulted of antigen with Ig bound by nonimmune mechanisms. MenosA better method for diagnosis of bovine trichomoniasis is needed because culture is slow and somewhat lacking in sensitivity. Immunodiagnosis of Tritrichomonas foetus infection usually involves detection of antigen-antibody reactions with an anti-immunoglobulin conjugate. However, nonspecific immunoglobulin (Ig) bound to the surface of T. foetus, would also be tetected by anti-Ig conjugate and thus would interfere with the specificity of the immunoassay. The goals of this study were to define the binding of bovine immunoglobulins to T. foetus. To determine whether nonimmune binding of Ig to T. foetus occurs, we immunized rabbits with organisms that had been grown in medium containing normal bovine serum and vigorously washed three times with phosphate-buffered saline. The rabbit antiserum had similar titers to T. foetus and to normal bovine serum by enzyme-linked immunosorbent assay (ELISA). Furthermore, two bovine serum proteins were immunoprecipitated by the rabbit antiserum in an immunoelectrophoretogram. One of the serum proteins had a distribution characteristics of IgG2. The rabbit antiserum was then shown to react with purified bovine IgG and IgM by ELISA. Reactivity to IgG was greater. To identify the IgG subisotypes bound and to confirm nonimmune binding of Ig, we grew T. foetus in agammaglobulinemic fetal calf serum and reacted it with IgG1, IgG2 and IgM specific for dinitrophenol (DNP) in ELISA. The binding of IgG2 was greastest that of IgG1 was next, and that of ... Mostrar Tudo |
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Palavras-Chave: |
Disease; Doencas da reproducao; Trichomonas faetus. |
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Thesagro: |
Reprodução. |
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Thesaurus Nal: |
reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02551naa a2200205 a 4500
001 1784150
005 2017-04-04
008 1991 bl --- 0-- u #d
100 1 $aCORBEIL, L. B.
245 $aImmunoglobulin binding by Tritrichomonas foetus.
260 $c1991
520 $aA better method for diagnosis of bovine trichomoniasis is needed because culture is slow and somewhat lacking in sensitivity. Immunodiagnosis of Tritrichomonas foetus infection usually involves detection of antigen-antibody reactions with an anti-immunoglobulin conjugate. However, nonspecific immunoglobulin (Ig) bound to the surface of T. foetus, would also be tetected by anti-Ig conjugate and thus would interfere with the specificity of the immunoassay. The goals of this study were to define the binding of bovine immunoglobulins to T. foetus. To determine whether nonimmune binding of Ig to T. foetus occurs, we immunized rabbits with organisms that had been grown in medium containing normal bovine serum and vigorously washed three times with phosphate-buffered saline. The rabbit antiserum had similar titers to T. foetus and to normal bovine serum by enzyme-linked immunosorbent assay (ELISA). Furthermore, two bovine serum proteins were immunoprecipitated by the rabbit antiserum in an immunoelectrophoretogram. One of the serum proteins had a distribution characteristics of IgG2. The rabbit antiserum was then shown to react with purified bovine IgG and IgM by ELISA. Reactivity to IgG was greater. To identify the IgG subisotypes bound and to confirm nonimmune binding of Ig, we grew T. foetus in agammaglobulinemic fetal calf serum and reacted it with IgG1, IgG2 and IgM specific for dinitrophenol (DNP) in ELISA. The binding of IgG2 was greastest that of IgG1 was next, and that of IgM was least. Little competitive inhibition by DNP was detected, indicating that binding of DNP-specific antibodies was predominantly nonimmune rather than antigen-specific Ig binding. We also demosntrated that T. foetus grew well in medium containing agammaglobulinemic fetal calf serum or serum made agammaglobulinemic by ammonium sulfate precipitations of Igs. This may overcome the problem of low specificity in diagnostic assays as a resulted of antigen with Ig bound by nonimmune mechanisms.
650 $areproduction
650 $aReprodução
653 $aDisease
653 $aDoencas da reproducao
653 $aTrichomonas faetus
700 1 $aHODGSON, J. L.
700 1 $aWIDDERS, P. R.
773 $tJournal of Clinical Microbiology$gv.29, n.12, p.2710-2714, 1991.
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
01/11/2017 |
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Data da última atualização: |
01/11/2017 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
FERREIRA, L. de S. |
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Afiliação: |
UFRRJ. |
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Título: |
Potencial de espécies locais na diversificação dos agroecossistemas, como culturas de cobertura, no manejo de plantas espontâneas. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
2016. |
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Páginas: |
61 p. |
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Idioma: |
Português |
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Notas: |
Dissertação. (Mestrado em Agricultura Orgância) - Universdiade Federal Rural do Rio de Janeiro, Seropédica. Orientação de Mariella Camardelli Uzeda. |
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Palavras-Chave: |
Atlantic forest; Desenvolvimento de plântulas; Diodia Saponariifolia; Mata Atlântica; Matocompetição; Poaia do brejo; Rio Guapi Macabu; Xique xique. |
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Thesagro: |
Cyperaceae. |
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Thesaurus NAL: |
Crotalária incana; Plant development; Poaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00922nam a2200265 a 4500
001 2078705
005 2017-11-01
008 2016 bl uuuu m 00u1 u #d
100 1 $aFERREIRA, L. de S.
245 $aPotencial de espécies locais na diversificação dos agroecossistemas, como culturas de cobertura, no manejo de plantas espontâneas.
260 $a2016.$c2016
300 $a61 p.
500 $aDissertação. (Mestrado em Agricultura Orgância) - Universdiade Federal Rural do Rio de Janeiro, Seropédica. Orientação de Mariella Camardelli Uzeda.
650 $aCrotalária incana
650 $aPlant development
650 $aPoaceae
650 $aCyperaceae
653 $aAtlantic forest
653 $aDesenvolvimento de plântulas
653 $aDiodia Saponariifolia
653 $aMata Atlântica
653 $aMatocompetição
653 $aPoaia do brejo
653 $aRio Guapi Macabu
653 $aXique xique
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