Data/hora: 07/06/2024 13:24:52

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 10/08/2004
Data da última atualização: | 10/04/2024
Autoria: | SILVA, J. R. V.; HURK, R. van den; COSTA, S. H. F.; ANDRADE, E. R.; NUNES, A. P. A.; FERREIRA, F. V. A.; LOBO, R. N. B.; FIGUEIREDO, J. R.
Afiliação: | JOSÉ R. V. SILVA, UNIVERSIDADE ESTADUAL DO CEARÁ; ROBERT VAN DEN HURK, UTRECHT UNIVERSITY; SONIA H. F. COSTA, UNIVERSIDADE ESTADUAL DO CEARÁ; EVELYN R. ANDRADE, UNIVERSIDADE ESTADUAL DO CEARÁ; RAIMUNDO NONATO BRAGA LOBO, CNPC.
Título: | Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water.
Ano de publicação: | 2004
Fonte/Imprenta: | Animal Reproduction Science, v. 81, n. 3/4, p. 273-286, 2004.
DOI: | 10.1016/j.anireprosci.2003.09.006
Idioma: | Inglês
Conteúdo: | Abstract: The development of culture systems to support the initiation of growth of primordial follicles is iniportant to the study of the factors that control the earliest stages of folliculogenesis. We investigated the effectiveness of five culture media, two supplements and three culture periods on the survival and growth of goal primordial follicles after culturing ovarian cortex. The media were based on minimal essential minimum (MEM) and coconut water solution (CWS) added in the proportion of 0, 25, 50, 75 or 100%. The two supplements were none versus supplemented with insulin-transferrin--selenium, pyruvate, glutamin (PCNA). The number of follicles in each stage and degenerated follicles were statistically analyzed by ANOVA using a factorial design and the significance of differences assessed using Tukey test. Chi-square test was used to compare the per?ntage of follicles with PCNA positive granulosa cells. As the culture period progressed, the number of primordial follicles fell and there was a significant increase in the number of primary follicles. The fall in the number of primordial follicles was particularly marked after 1 day culture. No effect of media on the number of primordial and primary follicles was observed after culture, but MEM as weil as supplements increased the number of intermediate follicles. Follicular degeneration was kept at the same level after culture in the media tested. except for pure CWS that increased the number of degenerated follicles. ln contrast, addition of supplements to culture media reduced follicular degeneration. ln non-cultured tissue, PCNA was expressed in granulosa cells of 31.6% of the growing follicles. This percentage had not significantly changed after 5 days culture in the various media, indicating the maintenance of proliferation activity of granulosa cens during culture. ln conclusion, it is shown that goat primordial follicles may be successfully activated after in vitro culture in aIl media tested. However, when pure CWS is used the follicular degeneration is enhanced, but the addition of supplements to culture media decrease follicular degeneration.
Palavras-Chave: | Activation; Drug effects; Folículo ovariano; Folículo primordial; Growth; Maintenance; Ovary; Primordial follicles; Suplemento; Supplements.
Thesagro: | Água de Coco; Caprino; Ovário; Reprodução.
Thesaurus Nal: | Bovine serum albumin; Cell division; Coconut water; Females; Glutamine; Goats; Granulosa cells; Hypoxanthine; In vitro culture; Insulin; Oocytes; Ovarian follicles; Pharmacology; Physiology; Reproduction; Solutions; Transferrin.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (17063-1) - UPC - AP - DD -  -  -  - DIS