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| Registros recuperados : 62 | |
| 22. |  | PINTO, P. P.; KATTAH, L. da S.; SA, N. M. H. de. Termotolerância e eficiência em fixar N2, de estirpes de Rhizobium nativas, isoladas de áreas de cultivos do feijoeiro nos cerrados. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.1, p. 509-510.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 27. | | OLIVEIRA JUNIOR, C. S.; CARNEIRO, J. A.; PURCINO, H.; VIANNA, M. C.; VARGAS, M. A.; SA, N. M. H de. Ocorrencia e efetividade de fixacao de N2 de estirpes nativas de rizobio associadas a Arachis pintoi, presentes em solos de cerrado. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 26., 1997, Rio de Janeiro. Informacao, globalizacao, uso do solo: anais. Rio de Janeiro: SBCS, 1997. p.38-41.| Biblioteca(s): Embrapa Cerrados. |
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| 28. | | SA, N. M. H. de; SCOTTI, M. R. M. M. L.; PAIVA, E.; FRANCO, A. A.; DOBEREINER, J. Selection and characterization of Rhizobium spp. strains stable and capable in fixing nitrogen in bean (Phaseolus vulgaris L.). Revista de Microbiologia, São Paulo, v. 24, n. 1, p. 38-48, 1993.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 29. | | OLIVEIRA JUNIOR, C. S.; SA, N. M. H.; PURCINO, H.; VASCONCELLOS, C. A.; VIANNA, M. C.; VARGAS, M. Efetividade de fixação de nitrogênio de estirpes nativas de rizobio associadas a Arachis pintoi isoladas de solos de cerrado. In: SEMANA DE INICIAÇÃO CIENTÍFICA, 5., 2009, Belo Horizonte. Anais... Belo Horizonte: UFMG, 1996. p. 54.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 30. | | SA, N. M. H. de; SILVA-KATTAH, L da; SELDIN, L.; VASCONCELOS, M. J. V.; PAIVA, E. Genomic heterogeneity within bean nodulating Rhizobium strains isolated from cerrado soils. Soil Biology and Biochemistry, Oxford, v. 29, n. 5/6, p. 1011-1014, 1997.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 31. | | RAPOSEIRAS, R.; PASSOS, R. V. M.; VARGAS, M. A. T.; PAIVA, E.; MARRIEL, I. E.; SA, N. M. H. Identificação de estirpes de Rhizobium competitivas para nodulação efetiva do feijoeiro nos cerrados. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 20., out. 1999, Salvador. Resumos... Salvador: SBM, 1999.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 32. | | PINTO, P. P.; CARNEIRO, J. A.; VARGAS, M. A. T.; PURCINO, H. A.; SA, N. M. H. Indigenous rhizobia associated with Arachis pintoi in cerrado soils of Brazil. Pasturas Tropicales, Cali, v. 21, n. 2, p. 25-28, 1999.| Biblioteca(s): Embrapa Cerrados. |
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| 34. | | OLIVEIRA, C. A.; SCOTTI, M. R. M. M.; PURCINO, H. A.; VASCONCELLOS, C. A.; RAPOSEIRAS, R.; SA, N. M. H. Decomposicao de Arachis pintoi em consorcio com gramineas forrageiras em area de cerrado na estacao seca. In: REUNIAO BRAS. FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 25.; REUNIAO BRASILEIRA SOBRE MICORRIZAS,8.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 6.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 3., 2000, Santa Maria, RS. Fertbio 2000: biodinamica do solo. [S.l.: s.n.], 2000. nao paginado.| Biblioteca(s): Embrapa Cerrados. |
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| 35. | | OLIVEIRA, C. A. de; MUZZI, M. R. S.; PURCINO, H. A.; MARRIEL, I. E.; SÁ, N. M. H. de. Decomposition of Arachis pintoi and Hyparrhenia rufa litters in monoculture and intercropped systems under lowland soil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 9, p. 1089-1095, set. 2003 ítulo em português: Decomposição da serrapilheira de Arachis pintoi e Hyparrhenia rufa em sistemasde monocultura e consórcio sob solo de várzea.| Biblioteca(s): Embrapa Algodão; Embrapa Unidades Centrais. |
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| 36. | | OLIVEIRA-PAIVA, C. A.; MUZZI, M. R. S.; PURCINO, H. A.; MARRIEL, I. E.; SA, N. M. H. de. Decomposition of Arachis pintoi and Hyparrhenia rufa litters in monoculture and intercropped systems under lowland soil. Pesquisa Agropecuária Brasileira, Brasília, v. 38, n. 9, p. 1089-1095, set. 2003.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 37. | | OLIVEIRA, I. A. de; VASCONCELLOS, M. J.; SELDIN, L.; PAIVA, E.; VARGAS, M. A.; SA, N. M. H. de. Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in brazilian cerrado soils. Brazilian Journal of Microbiology, Sao Paulo, v. 31, p. 39-44, 2000.| Biblioteca(s): Embrapa Cerrados. |
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| 38. | | OLIVEIRA, I. A. de; VASCONCELOS, M. J.; SELDIN, L.; PAIVA, E.; VARGAS, M. A.; SA, N. M. H. de. Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in Brazilian cerrado soils. Brazilian Journal of Microbiology, São Paulo, v.31, n. 1, p. 39-44, 2000.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 39. | | OLIVEIRA-PAIVA, C. A.; VASCONCELLOS, C. A.; MARRIEL, I. E.; PEREIRA FILHO, I. A.; SA, N. M. H. de. Efeito da temperatura sobre a fixação de N2 do feijoeiro. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 23.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 7.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 5.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 2., 1998. Caxambu, MG. FertBIO 98: resumos. Lavras: UFLA: SBCS: SBM, 1998. p. 181.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 40. | | RAPOSEIRAS, R.; PASSOS, R.; CARVALHO, L.; PAIVA, E.; MARRIEL, I. E.; PEREIRA, I.; MENDES, I.; SA, N. M. H. Capacidade competitiva de estirpes efetivas de Rhizobium na nodulação do feijoeiro nos cerrados. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 28., 2001, Londrina. Ciência do solo: fator de produtividade competitiva com sustentabilidade. Londrina: SBCS, 2001. p. 93.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 62 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/04/2010 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VIREQUE, A. A.; CAMARGO, L. S. de A.; SERAPIÃO, R. V.; ROSA E SILVA, A. A. M.; WATANABE, Y. F.; FERREIRA, E. M.; NAVARRO, P. A. A. S.; MARTINS, W. P.; FERRIANI, R. A. |
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Afiliação: |
A. A. VIREQUE, USP; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFRRJ; A. A. M. ROSA E SILVA, Universidade de Brasília; Y. G. WATANABE, Vitrogen; E. M. FERREIRA, USP; P. A. A. S. NAVARRO, USP; W. P. MARTINS, USP; R. A. FERRIANI, USP. |
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Título: |
Preimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Theriogenology, v. 71, n. 4, p. 620-627, 2009. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2008.09.028 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis. MenosIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in ... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; In vitro maturation. |
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Thesaurus NAL: |
embryogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711876/1/Preimplantation-development-and-expression-of-Hsp-70-and-Bax-genes-in-bovine-blastocysts.pdf
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Marc: |
LEADER 02626naa a2200265 a 4500
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024 7 $ahttps://doi.org/10.1016/j.theriogenology.2008.09.028$2DOI
100 1 $aVIREQUE, A. A.
245 $aPreimplantation development and expression of Hsp-70 and Bax genes in bovine blastocysts derived from oocytes matured in alpha-MEM supplemented with growth factors and synthetic macromolecules.
260 $c2009
520 $aIn vitro culture conditions affect both the maternal and embryonic expression of genes and is likely to alter both oocyte and embryo developmental competence. The search for better and less variable culture conditions simulating those in vivo has led to the development of defined culture media, with lower impact on the molecular reprogramming of oocytes and embryos. We evaluated embryo development and relative abundance (RA) of Hsp-70 and Bax transcripts in bovine blastocysts produced from oocytes matured in a chemically defined IVM system with synthetic polymers. Immature cumulus oocyte complexes (COCs) were matured for 22-24h in alpha-MEM supplemented with IGF-1, insulin, 0.1% polyvinyl alcohol (PVA), or 0.1% polyvinylpyrrolidone (PVP), but without FSH or LH. The control group consisted of COCs matured in TCM plus FSH and 10% estrous cow serum. After fertilization, presumptive zygotes were co-cultured with cumulus cells until 224 h post-insemination. Total RNA was isolated from embryo pools, reverse transcribed into cDNA, and subjected to transcript analysis by real-time PCR. Cleavage rate was higher (P<0.05) for the control group (68.3%) than for the PVA (54.4%) and PVP-40 (58.3%) groups. Nevertheless, there was no difference among the PVA, PVP-40 and control groups in blastocyst or hatching rates. Similarly, no difference in relative abundance of Hsp-70 and Bax transcripts was detected in comparison to the control group. We inferred that bovine oocytes can be matured in serum- and gonadotrophin-free medium supplemented with PVA or PVP, enriched with IGF-I and insulin, without altering post-cleavage development and relative abundance of some genes associated with stress and apoptosis.
650 $aembryogenesis
653 $aBovine oocytes
653 $aIn vitro maturation
700 1 $aCAMARGO, L. S. de A.
700 1 $aSERAPIÃO, R. V.
700 1 $aROSA E SILVA, A. A. M.
700 1 $aWATANABE, Y. F.
700 1 $aFERREIRA, E. M.
700 1 $aNAVARRO, P. A. A. S.
700 1 $aMARTINS, W. P.
700 1 $aFERRIANI, R. A.
773 $tTheriogenology$gv. 71, n. 4, p. 620-627, 2009.
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