| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
17/02/2016 |
|
Data da última atualização: |
17/02/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MÜLLER, B. S. F.; PAPPAS JUNIOR, G. J.; VALDISSER, P. A. M. R.; COELHO, G. R. C.; MENEZES, I. P. P. de; ABREU, A. G.; BORBA, T. C. O.; SAKAMOTO, T.; BRONDANI, C.; BARROS, E. G.; VIANELLO, R. P. |
|
Afiliação: |
BARBARA S. F. MÜLLER, BIOAGRO; GEORGIOS J. PAPPAS JUNIOR, UNB; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; IVANDILSON P. P. DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; ALUANA GONCALVES DE ABREU, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; TETSU SAKAMOTO, UFMG; CLAUDIO BRONDANI, CNPAF; EVERALDO G. BARROS, BIOAGRO; ROSANA PEREIRA VIANELLO, CNPAF. |
|
Título: |
An operational SNP panel integrated to SSR marker for the assessment of genetic diversity and population structure of the common bean. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 33, n. 6, p. 1697-1711, Dec. 2015. |
|
DOI: |
10.1007/s11105-015-0866-x |
|
Idioma: |
Inglês |
|
Conteúdo: |
The common bean, an important source of protein and minerals for humans, complements cereals both nutritionally and as a rotation crop, supplying nitrogen and reducing soil pathogens. The aim of this study was to develop an operational SNP-based panel for common bean in order to facilitate SSR employment in genetic diversity and population structure analyses, and its use in breeding programs. A set of 88 diverse and important common bean cultivars/lines (53), landraces (33) and wild accessions (2) were genotyped. Overall, the 58 SSRs performed better at evaluating genetic diversity (Ā=7.38; He=58.7 %; PI=1.20E−45) than the 345 SNPs, of which the SSRs dinucleotides (SSR-di) were more informative (Ā=9.92; He=72.5 %; PI=3.40E−26) and a selected set of 13 SSRs (Ā=15.31/locus; He=84.5 %; PI=1.03E−19) allowed for the discrimination of all individuals. For the 345 high-quality scored SNPs a low combined PI (4.70E −119) and high PE (100 %) was obtained for the assessment of parentage and identity. The SNPs were very useful for linkage mapping in inter- (78.2 %) and intra-gene pool (17.7 %) crosses. Both markers afforded high resolution detection of inter-gene pool structure, with greater differentiation based on SNPs (K=2, FST=0.759). The SSRs-di differentiated cultivars/lines and landraces (K=3) of Mesoamerican origin. A set of 16 SSRs was selected to establish a routine and operational analysis of Genbank accessions allowing an efficient origin-based discrimination of common bean accessions. Operational genotyping panels based on SSRs and SNPs were derived, contributing to the growing integration of genomics with molecular breeding programs of the common bean. MenosThe common bean, an important source of protein and minerals for humans, complements cereals both nutritionally and as a rotation crop, supplying nitrogen and reducing soil pathogens. The aim of this study was to develop an operational SNP-based panel for common bean in order to facilitate SSR employment in genetic diversity and population structure analyses, and its use in breeding programs. A set of 88 diverse and important common bean cultivars/lines (53), landraces (33) and wild accessions (2) were genotyped. Overall, the 58 SSRs performed better at evaluating genetic diversity (Ā=7.38; He=58.7 %; PI=1.20E−45) than the 345 SNPs, of which the SSRs dinucleotides (SSR-di) were more informative (Ā=9.92; He=72.5 %; PI=3.40E−26) and a selected set of 13 SSRs (Ā=15.31/locus; He=84.5 %; PI=1.03E−19) allowed for the discrimination of all individuals. For the 345 high-quality scored SNPs a low combined PI (4.70E −119) and high PE (100 %) was obtained for the assessment of parentage and identity. The SNPs were very useful for linkage mapping in inter- (78.2 %) and intra-gene pool (17.7 %) crosses. Both markers afforded high resolution detection of inter-gene pool structure, with greater differentiation based on SNPs (K=2, FST=0.759). The SSRs-di differentiated cultivars/lines and landraces (K=3) of Mesoamerican origin. A set of 16 SSRs was selected to establish a routine and operational analysis of Genbank accessions allowing an efficient orig... Mostrar Tudo |
|
Palavras-Chave: |
Diversity pattern; Genetic structure; Molecular breeding. |
|
Thesagro: |
Feijão; Phaseolus vulgaris; Variação genética. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02750naa a2200325 a 4500
001 2037381
005 2016-02-17
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s11105-015-0866-x$2DOI
100 1 $aMÜLLER, B. S. F.
245 $aAn operational SNP panel integrated to SSR marker for the assessment of genetic diversity and population structure of the common bean.$h[electronic resource]
260 $c2015
520 $aThe common bean, an important source of protein and minerals for humans, complements cereals both nutritionally and as a rotation crop, supplying nitrogen and reducing soil pathogens. The aim of this study was to develop an operational SNP-based panel for common bean in order to facilitate SSR employment in genetic diversity and population structure analyses, and its use in breeding programs. A set of 88 diverse and important common bean cultivars/lines (53), landraces (33) and wild accessions (2) were genotyped. Overall, the 58 SSRs performed better at evaluating genetic diversity (Ā=7.38; He=58.7 %; PI=1.20E−45) than the 345 SNPs, of which the SSRs dinucleotides (SSR-di) were more informative (Ā=9.92; He=72.5 %; PI=3.40E−26) and a selected set of 13 SSRs (Ā=15.31/locus; He=84.5 %; PI=1.03E−19) allowed for the discrimination of all individuals. For the 345 high-quality scored SNPs a low combined PI (4.70E −119) and high PE (100 %) was obtained for the assessment of parentage and identity. The SNPs were very useful for linkage mapping in inter- (78.2 %) and intra-gene pool (17.7 %) crosses. Both markers afforded high resolution detection of inter-gene pool structure, with greater differentiation based on SNPs (K=2, FST=0.759). The SSRs-di differentiated cultivars/lines and landraces (K=3) of Mesoamerican origin. A set of 16 SSRs was selected to establish a routine and operational analysis of Genbank accessions allowing an efficient origin-based discrimination of common bean accessions. Operational genotyping panels based on SSRs and SNPs were derived, contributing to the growing integration of genomics with molecular breeding programs of the common bean.
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aVariação genética
653 $aDiversity pattern
653 $aGenetic structure
653 $aMolecular breeding
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aVALDISSER, P. A. M. R.
700 1 $aCOELHO, G. R. C.
700 1 $aMENEZES, I. P. P. de
700 1 $aABREU, A. G.
700 1 $aBORBA, T. C. O.
700 1 $aSAKAMOTO, T.
700 1 $aBRONDANI, C.
700 1 $aBARROS, E. G.
700 1 $aVIANELLO, R. P.
773 $tPlant Molecular Biology Reporter$gv. 33, n. 6, p. 1697-1711, Dec. 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
27/10/2008 |
|
Data da última atualização: |
03/04/2017 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
VESCHI, J. L. A.; DUTRA, I. S.; ALVES, M. A. B.; FERNANDEZ-MIYAKAWA, M. E.; PERRI, S. H. V.; UZAL, F. A. |
|
Afiliação: |
JOSIR LAINE APARECIDA VESCHI, CPATSA; Iveraldo S. Dutra, UNESP; Maria Angélica B. Alves, FAPESP; Mariano E. Fernandez-Miyakawa, UCDavis; Silvia Helena V. Perri, UNESP; Francisco A. Uzal, UCDavis. |
|
Título: |
Sorological evaluation of commercial vaccines against enterotoxemia in goats. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23, 2008, Querétaro, México. Sustainable goat production: challenges an opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association, 2008. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Enterotoxemia in sheep and in goats is caused by the effects of the epsilon toxin of C/ostridium perfringens type D, being considered the main infectious cause of mortality in those animal species. The main prophylactic measures include adequate nutritional management and vaccination of ali animais using vaccines of high immunogenic power. Six commercial vaccines containing in its formulation the epsilon toxoid of C. perfringens type D were sorogically evaluated. Eighty four female goat kids, whose mothers had no previous vaccination history against clostridioses were used. They were divided into six groups of 14 animais each. The animais of the control group didn't receive any vaccine dose and the animais from the groups 1 to 5 received two vaccine doses, The first vaccine dose was applied at 45 days of life (day zero) and the second dose at 75 days (30 days after the first dose). Blood samples were collected from the goat kids at the days zero, 30, 60, 90, 120 and 150 after the beginning of the experiment, in order to evaluate the immunologic response. The Indirect ELlSA technique was used for the quantification of the antibodies against epsilon toxin in the samples of blood serum of the animais. In day zero, no animal presented titre considered protector. The largest number of animais considered protected was found at day 60, in response to the two initial doses of the vaccine (days O and 30, first and second doses, respectively). Only tive animaIs which received the vaccine 1 and one animal which received the vaccine 3 stayed wilh titres of antibodies considered up to 150 days after the first vaccine dose. Based on the results, it was concluded lhat the evaluated vaccines showed small amount of epsilon toxoid in the commercial formulations, a crucial fact for lhe low efficiency of. the vaccines. For commercial reasons, the vaccines against the clostridioses present versatile formulations, with several toxoid types, used for various animal species, which certainly contributed to reduce their effectiveness in preventing the iIInesses caused by the clostridia or their toxins. MenosEnterotoxemia in sheep and in goats is caused by the effects of the epsilon toxin of C/ostridium perfringens type D, being considered the main infectious cause of mortality in those animal species. The main prophylactic measures include adequate nutritional management and vaccination of ali animais using vaccines of high immunogenic power. Six commercial vaccines containing in its formulation the epsilon toxoid of C. perfringens type D were sorogically evaluated. Eighty four female goat kids, whose mothers had no previous vaccination history against clostridioses were used. They were divided into six groups of 14 animais each. The animais of the control group didn't receive any vaccine dose and the animais from the groups 1 to 5 received two vaccine doses, The first vaccine dose was applied at 45 days of life (day zero) and the second dose at 75 days (30 days after the first dose). Blood samples were collected from the goat kids at the days zero, 30, 60, 90, 120 and 150 after the beginning of the experiment, in order to evaluate the immunologic response. The Indirect ELlSA technique was used for the quantification of the antibodies against epsilon toxin in the samples of blood serum of the animais. In day zero, no animal presented titre considered protector. The largest number of animais considered protected was found at day 60, in response to the two initial doses of the vaccine (days O and 30, first and second doses, respectively). Only tive animaIs which received the vacc... Mostrar Tudo |
|
Palavras-Chave: |
Avaliação comercial; Clostridium perfringens type D; Epsilon toxin. |
|
Thesagro: |
Caprino; Doença; Enterotoxemia; Imunologia; Produção animal; Toxina; Vacina. |
|
Thesaurus NAL: |
Immune response; Vaccination. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03183nam a2200313 a 4500
001 1160900
005 2017-04-03
008 2008 bl uuuu u00u1 u #d
100 1 $aVESCHI, J. L. A.
245 $aSorological evaluation of commercial vaccines against enterotoxemia in goats.
260 $aIn: INTERNATIONAL CONFERENCE ON GOATS, 9.; REUNIÓN NACIONAL SOBRE CAPRINOCULTURA, 23, 2008, Querétaro, México. Sustainable goat production: challenges an opportunities of small and large enterprises; proceedings. Querétaro: International Goat Association$c2008
520 $aEnterotoxemia in sheep and in goats is caused by the effects of the epsilon toxin of C/ostridium perfringens type D, being considered the main infectious cause of mortality in those animal species. The main prophylactic measures include adequate nutritional management and vaccination of ali animais using vaccines of high immunogenic power. Six commercial vaccines containing in its formulation the epsilon toxoid of C. perfringens type D were sorogically evaluated. Eighty four female goat kids, whose mothers had no previous vaccination history against clostridioses were used. They were divided into six groups of 14 animais each. The animais of the control group didn't receive any vaccine dose and the animais from the groups 1 to 5 received two vaccine doses, The first vaccine dose was applied at 45 days of life (day zero) and the second dose at 75 days (30 days after the first dose). Blood samples were collected from the goat kids at the days zero, 30, 60, 90, 120 and 150 after the beginning of the experiment, in order to evaluate the immunologic response. The Indirect ELlSA technique was used for the quantification of the antibodies against epsilon toxin in the samples of blood serum of the animais. In day zero, no animal presented titre considered protector. The largest number of animais considered protected was found at day 60, in response to the two initial doses of the vaccine (days O and 30, first and second doses, respectively). Only tive animaIs which received the vaccine 1 and one animal which received the vaccine 3 stayed wilh titres of antibodies considered up to 150 days after the first vaccine dose. Based on the results, it was concluded lhat the evaluated vaccines showed small amount of epsilon toxoid in the commercial formulations, a crucial fact for lhe low efficiency of. the vaccines. For commercial reasons, the vaccines against the clostridioses present versatile formulations, with several toxoid types, used for various animal species, which certainly contributed to reduce their effectiveness in preventing the iIInesses caused by the clostridia or their toxins.
650 $aImmune response
650 $aVaccination
650 $aCaprino
650 $aDoença
650 $aEnterotoxemia
650 $aImunologia
650 $aProdução animal
650 $aToxina
650 $aVacina
653 $aAvaliação comercial
653 $aClostridium perfringens type D
653 $aEpsilon toxin
700 1 $aDUTRA, I. S.
700 1 $aALVES, M. A. B.
700 1 $aFERNANDEZ-MIYAKAWA, M. E.
700 1 $aPERRI, S. H. V.
700 1 $aUZAL, F. A.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
