Data/hora: 08/06/2024 21:00:28

Biblioteca(s): 	Embrapa Pecuária Sudeste.	
Data corrente: 	03/04/2019	
Data da última atualização: 	20/11/2019	
Autoria: 	GRANJA-SALCEDO, Y. T.; FERNANDES, R. M.; ARAUJO, R. C. de; KISHI, L. T.; BERCHIELLI, T. T.; RESENDE, F. D. de; BERNDT, A.; SIQUEIRA, G. R.	
Afiliação: 	Yury Tatiana Granja-Salcedo, UNESP; Rodolfo Maciel Fernandes, UNESP/Agência Paulista de Tecnologia dos Agronegócios; Rafael Canonenco de Araujo, GRASP; Luciano Takeshi Kishi, UNESP; Telma Teresinha Berchielli, UNESP; Flávio Dutra de Resende, UNESP; ALEXANDRE BERNDT, CPPSE; Gustavo Rezende Siqueira, UNESP/Agência Paulista de Tecnologia dos Agronegócios.	
Título: 	Long-Term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Frontiers in microbiology, v. 10, n. 614, p. 1-12, 2019.	
Idioma: 	Inglês	
Conteúdo: 	This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (C48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (- 9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactateproducer bacteria, thereby reducing acetate production during rumen fermentation.	
Palavras-Chave: 	Archaea diversity; Enteric methane emission.	
Thesagro: 	Gado de Corte; Nitrato.	
Thesaurus Nal: 	Beef cattle; Fatty acids; Rumen bacteria.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/195343/1/Long-Term-Encapsulated-Nitrate.pdf	
Registro original: 	Embrapa Pecuária Sudeste (CPPSE)	
Exemplares: 	CPPSE - (24752-1) - UPC - AP - DD - PROCI-2019.00019 - GRA - 2019.00020 - DIS