Data/hora: 18/06/2024 02:20:39

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 13/08/2021
Data da última atualização: | 13/08/2021
Autoria: | SANTOS, T. R.; PAIXÃO, F. R. S. da; CATÃO, A. M. L.; MUNIZ, E. R.; RIBEIRO-SILVA, C. S.; TAVEIRA, S. F.; LUZ, C.; MASCARIN, G. M.; FERNANDES, E. K. K.; MARRETO, R. N.
Afiliação: | THAINÁ RODRIGUES SANTOS, UFG; FLÁVIA REGINA SANTOS DA PAIXÃO, UFG; ALAINE MARIA LOPES CATÃO, UFG; ELEN REGOZINO MUNIZ, UFG; CÁRITA SOUZA RIBEIRO-SILVA, UFG; STEPHANIA FLEURY TAVEIRA, UFG; CHRISTIAN LUZ, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG; RICARDO NEVES MARRETO, UFG.
Título: | Inorganic pellets containing microsclerotia of Metarhizium anisopliae: a new technological platform for the biological control of the cattle tick Rhipicephalus microplus.
Ano de publicação: | 2021
Fonte/Imprenta: | Applied Microbiology and Biotechnology, V. 105, N. 12, p. 5001-5012, 2021.
ISSN: | 1432-0614
DOI: | https://doi.org/10.1007/s00253-021-11372-1
Idioma: | Inglês
Conteúdo: | Abstract: This study was sought to devise pellets containing inorganic materials and microsclerotia of Metarhizium anisopliae strain IP 119 for biological control of Rhipicephalus microplus, the most economically important tick in Brazilian cattle industry. In addition, we evaluated the storage stability of the pellets, their tolerance to ultraviolet radiation (UV-B), and efficacy against ticks under laboratory conditions. Fungal microsclerotia were produced by liquid culture fermentation and mixed with pre-selected inorganic matrices: vermiculite powder, diatomaceous earth, and colloidal silicon dioxide (78:20:2, w/w/w). The microsclerotial pellets were then prepared by a two-stage process involving extrusion and spheronization. Pellet size averaged 525.53 ± 7.74 micrometers, with a sphericity index of 0.72 ± 0.01, while biomass constituents did not affect the wet mass properties. Conidial production from microsclerotial pellets upon rehydration ranged from 1.85 × 10^9 to 1.97 × 10^9 conidia g-1 with conidial viability >= 93%. Conidial production from pellets stored at 4 °C was invariable for up to 21 days. Unformulated microsclerotia and microsclerotial pellets were extremely tolerant to UV-B compared with aerial conidia. Engorged tick females exposed to conidia from sporulated pellets applied to soil samples and upon optimal rehydration exhibited shorter oviposition time length, shorter life span, and reduced number of hatched larvae. In summary, microsclerotial pellets of M. anisopliae IP 119 effectively suppressed R. microplus and showed outstanding UV-B tolerance in laboratory tests. Prospectively, this formulation prototype is promising for targeting the non-parasitic stage of this tick on outdoor pasture fields and may offer a novel mycoacaricide for its sustainable management.
Palavras-Chave: | Bioefficacy.
Thesagro: | Bovino; Carrapato; Controle Biológico; Fungo Entomógeno; Fungo Para Controle Biológico; Pellet.
Thesaurus Nal: | Biological control; Entomopathogenic fungi; Pellets; Rhipicephalus microplus; Ticks.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (17003-1) - UPC - AP - DD -  -  -  - DIS