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| Registros recuperados : 75 | |
| 2. |  | MIYAUCHI, C. A. C.; FERNANDES, C. A. C.; OLIVEIRA, E. R.; ALVES, B. F. L.; VIANA, J. H. M. Effect of follicular wave synchronization and absence of corpus luteum on coc recovery in gyr (Bos taurus indicus) cows. Reproduction, Fertility and Development, v. 22, n. 1, 2010. Edição dos proceedings do Annual Conference of the International Embryo, 2010, Cordoba.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | FERREIRA, A. M.; SA, W. F.; VIANA, J. H. M.; FERNANDES, C. A. C.; CAMARGO, L. S. A. Taxa de nao retorno ao cio em 60 dias e taxa de prenhez em bovinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 49, n. 2, p. 261-267, 1997.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | FERNANDES, C. A. C.; OLIVEIRA, E. R.; VASCONCELOS, T. D.; ALVES, B. F. L.; GIOSO, M. M.; VIANA, J. H. M.; OBA, E.; FIGUEIREDO, A. C. S. Bipartição de embriões bovinos em diferentes estádios de desenvolvimento. Acta Scientiae Veterinariae, v. 35, p. s1215, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | ROSSI, T. C.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VARAGO, F. C.; MIYAUCHI, T. M.; ROSSI, J. R.; GIOSO, M. M. Bovine oocyte transportation in environment with or without control gas atmosphere. Animal Reproduction, v. 10, n. 3, p. 547, Jul./Sept. 2013. Suplemento. Edição dos abstracts do 27º Annual Meeting of the Brazilian Embryo Technology Society, 2013, Praia do Forte, BA.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | GHETTI, A. M.; BRANDAO, F. Z.; SIQUEIRA, L. G. B.; AREAS, V. S.; ARASHIRO, E. K. N.; FERNANDES, C. A. C.; PALHAO, M. P.; VIANA, J. H. M. Comparação dos metodos e subjetivo de avaliação do padrão vascular do foliculo pre-ovulatorio utilizando-se Doppler colorido. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. p. 478.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; SOUZA, E. D.; ARASHIRO, E. K. N.; CAMARGO, L. S. A.; FONSECA, J. F. da; FERNANDES, C. A. C. Eficiência na indução da luteólise em diferentes dias após a coleta de embriões em bovinos (resultados parciais). Acta Scientiae Veterinariae, v. 35, p. s1230, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 9. | | SIQUEIRA, L. G. B.; TORRES, C. A. A.; AMORIM, L. S.; SOUZA, E. D.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle. Animal Reproduction Science, v. 115, n. 1/4, p. 18-28, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | GRÁZIA, J. G. V. de; ARASHIRO, E. K. N.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Is it possible to increase the efficiency of in vitro embryo production in Holstein preselecting donors with more oocyte production? Animal Reproduction, v.10, n.3, p. 526, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | VARAGO, F. C.; SILVA, L. P.; RIBEIRO, J. R.; FERNANDES, C. A. C.; CARVALHO, B. C. de; GIOSO, M. M.; GIMENEZ, M.; NEVES, J. P. Acrosome reaction induced by theophylline associated or not to heparin in bovine semen Animal Reproduction, v. 12, n. 3, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | VIANA, J. H. M.; PEREIRA, N. E. S.; FARIA, O. A. C.; DIAS, L. R. O.; OLIVEIRA, E. R.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B. Active immunization against GnRH as an alternative therapeutic approach for the management of Bos indicus oocyte donors diagnosed with chronic cystic ovarian disease. Theriogenology, v. 172, p. 133-141, 2021.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; DINIZ, E. S.; CAMARGO, L. S. A.; OLIVEIRA, A. P.; FERNANDES, C. A. C.; TORRES, C. A. A. Luteal tissue area in embryo recipients synchronized and with spontaneous or estradiol benzoate induced ovulation. Acta Scientiae Veterinariae, v. 34, s494, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | VIANA, J. H. M.; ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C. Manejo de riscos associados à aspiração folicular orientada por ultrassonografia em bovinos. Spermova, v. 3, n. 1, p. 11-14, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | FERNANDES, C. A. C.; OBA, E.; VIANA, J. H. M.; FIGUEIREDO, A. C. S.; OLIVEIRA, E. R.; VASCONCELOS, T. D.; GIOSO, M. M. Economic evaluation of bovine embryo splitting. Acta Scientiae Veterinariae, v. 34, s506, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | GUIMARÃES, C. R. B.; OLIVEIRA, M. E.; ROSSI, J. R.; FERNANDES, C. A. C.; VIANA, J. H. M.; PALHAO, M. P. Corpus luteum blood flow evaluation on Day 21 to improve the management of embryo recipient herds. Theriogenology, v. 84, n. 2, p. 237-241, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | FERNANDES, C. A. C.; OBA, E.; FIGUEIREDO, A. C. S.; OLIVEIRA, E. R.; ALVES, B. F. L.; VIANA, J. H. M. Perdas embrionárias e fetais em gestações gemelares de receptoras de embriões bovinos. Acta Scientiae Veterinariae, v. 35, p. s1074, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | VIANA, J. H. M.; DOREA, M. D.; SIQUEIRA, L. G. B.; ARASHIRO, E. K. N.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; PALHAO, M. P. Occurrence and characteristics of residual follicles formed after transvaginal ultrasound-guided follicle aspiration in cattle. Theriogenology, v. 79, n. 2, p. 267-273, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | LEAL, J. W. L.; SILVA, G. H.; SANTOS, D. B. dos; TEIXEIRA, W.; LIMA, M. I. C. de; FERNANDES, C. A. C.; PINTO, A. do C. Folha SC.20 Porto Velho. I - Geologia. In: BRASIL. Ministerio das Minas e Energia. Projeto RADAMBRASIL. Folha SC.20 Porto Velho: geologia, geomorfologia, pedologia, vegetacao, uso potencial da terra. Rio de Janeiro, 1978. p.19-184. 30 figuras, 16 fotografias, 51 tabelas, 1 mapa.| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | VIANA, J. H. M.; CAMARGO, L. S. A.; FONSECA, J. F.; OLIVEIRA, A. P.; ARASHIRO, E. K. N.; FREITAS, C.; FERNANDES, C. A. C. Retrospective analysis of cumulus-oocyte complexyield in a gyr (Bos Taurus Indicus) herd undergoing trasvaginal ultrasound-guided fillicle aspiration. In: REPRODUCTION, FERTILITY AND DEVELOPMENT. 20. 2008, Anais. Colorado. Csiro, Academy of Science, 2008. 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 75 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. |
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Afiliação: |
Flavio Guisseli Lopes; Eduardo Paulino da Costa; Vanessa Lopes Dias Queiroz-Castro; Emilio Cesar Martins Pereira; José Domingos Guimarães; Saullo Vinicius Pereira Alves; Carlos Antonio Carvalho Fernandes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Laercio dos Anjos Benjamim. |
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Título: |
Use of two new formulations as bovine embryo manipulation solution. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction, v. 16 n. 2, p. 348-355, 2019. |
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DOI: |
10.21451/1984-3143-ar2018-0044 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability. MenosABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS ... Mostrar Tudo |
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Palavras-Chave: |
Cell apoptosis; Hsp 70 ponto 3. |
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Thesaurus NAL: |
Mice. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208745/1/Use-of-two-new-formulations.pdf
|
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Marc: |
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001 2118720
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.21451/1984-3143-ar2018-0044$2DOI
100 1 $aLOPES, F. G.
245 $aUse of two new formulations as bovine embryo manipulation solution.$h[electronic resource]
260 $c2019
520 $aABSTRACT This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stages of early blastocyst, and compact morula grades I and II. These embryos were initially randomly distributed and maintained for four hours in three solutions: Modified phosphate buffered saline (PBS; Control); EMS (treatment 1), and EMS supplemented (treatment 2). Subsequently, they were cultured in TCM 199 medium and evaluated in terms of total number of cells, morphometric characteristics, ultra structural aspects, detection of cell apoptosis, and quantification of Hsp70.3 gene expression. In the second phase, these same solutions were tested in the transfer of quality I and II bovine embryos (excellent and good). These embryos were transferred fresh to 58 recipients. The results showed that the total number of cells in embryos expanded blastocyst (ExB), the number of apoptotic cells, the cell, nuclear, nucleolar diameter and the nucleus/nucleolus ratio was similar among the treatments. The pregnancy rate shown on second phase was also similar. However, the EMS supplemented expressed more Hsp70.3 than EMS. The expression of Hsp70.3 was also greater for embryos in EMS than that of EMS supplemented. The McII embryos, EMS and EMS supplemented samples also expressed more Hsp70.3 compared to control embryos. In conclusion, the tested solutions can be used in routine embryo transfer techniques, replacing modified PBS solution as an effective media in maintaining embryo viability.
650 $aMice
653 $aCell apoptosis
653 $aHsp 70 ponto 3
700 1 $aCOSTA, E. P. DA
700 1 $aQUEIROZ-CASTRO, V. L. D.
700 1 $aPEREIRA, E. C. M.
700 1 $aGUIMARÃES, J. D.
700 1 $aALVES, S. V. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBENJAMIM, L. DOS A.
773 $tAnimal Reproduction$gv. 16 n. 2, p. 348-355, 2019.
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Embrapa Gado de Leite (CNPGL) |
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