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| Registros recuperados : 75 | |
| 61. |  | PALHÃO, M. P.; GUIMARÃES, C. R. B.; LIMA, J. F. J. F. M.; MENDONÇA, M. R.; FERNANDES, C. A. C.; NEVES, J. P.; GARCIA, J. A. D.; GIOSO, M. M.; MIGLINO, M. A.; VIANA, J. H. M. Efficacy and limitations of different approaches to anticipate the diagnosis of pregnancy in cattle. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 71, n. 6, p. 1909-1916, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 62. | | PALHÃO, M. P.; RIBEIRO, A. C.; MARTINS, A. B.; GUIMARÃES, C. R. B.; ALVAREZ, R. D.; SEBER, M. F.; FERNANDES, C. A. C.; NEVES, J. P.; VIANA, J. H. M. Early resynchronization of non-pregnant beef cows based in corpus luteum blood flow evaluation 21 days after Timed-AI. Theriogenology, v. 146, p. 26-30, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 63. | | ARASHIRO, E. K. N.; SIGUEIRA, L. G. B.; VIANA, J. H. M.; FONSECA, J. F.; BRUSCHI, J. H.; CAMARGO, L. S. A.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Dinâmica luteal em cabras da raça toggenburg: caracterização morfológica e endócrina. Acta Scientiae Veterinariae, v. 36, p. s453, 2008. Supl. 2. Edição dos anais da 22ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 64. | | ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; FONSECA, J. F. da; BRUSCHI, J. H.; CAMARGO, L. S.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Dinâmica luteal em cabras da raça Toggenburg: caracterização morfológica e endócrina. Acta Scientiae Veterinariae Porto Alegre, v. 36, Supl. 2, p. s453, 2008. Edição dos Resumos XXII Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 65. | | VIANA, J. H. M.; ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; GHETTI, A. M.; AREAS, V. S.; GUIMARÃES, C. R. B.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C. Doppler ultrasonography as a tool for ovarian management. Animal Reproduction, v. 10, n. 3, p. 215-222, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 66. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. Theriogenology, v. 126, p. 68-74, 2019.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 67. | | MINARÉ, T. P.; ARASHIRO, E. K. N.; WOHLRES-VIANA, S.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CORDEIRO, C. E. C.; FERNANDES, C. A. C.; VIANA, J. H. M. Characterization of individual differences in the efficiency of superovulation in the Gyr breed. Animal Reproduction, v.10, n.3, p. 546, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 68. | | CANELLA FILHO, C. F. C.; FERNANDES, C. A. C.; VIANA, J. H. M.; FIGUEIREDO, A. C. S.; GIOSO, M. M.; LUDGERO, B. F. A.; OLIVEIRA, E. R.; VASCONCELOS, T. D. Intra-uterine treatment for infections in cows. Acta Scientiae Veterinariae, v. 34, s500, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 69. | | SIQUEIRA, L. G. B.; VIANA, J. H. M.; DINIZ, E. S.; CAMARGO, L. S. A.; AMORIM, L. S.; FONSECA, J. F.; FERNANDES, C. A. C.; TORRES, C. A. A. Luteal echogenicity evaluation using different ultrasound probes. Acta Scientiae Veterinariae, v. 34, s281, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Biblioteca(s): Embrapa Gado de Leite. |
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| 70. | | ARASHIRO, E. K. N.; VIANA, J. H. M.; FONSECA, J. F. da; SIQUEIRA, L. G. B.; BRUSCHI, J. H.; CAMARGO, L. S. A.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Luteal morphometric parameters and sonographic echotexture during luteogenesis period in pregnant goats. Reproduction, Fertility and Development, v. 20, n. 1, p. 236, 2008. Edição dos proceedings of the Annual Conference of the International, Embryo Society, Denver, Colorado, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 71. | | ARASHIRO, E. K. N.; VIANA, J. H. M.; OLIVEIRA, J. L.; SIQUEIRA, L. G. B.; BRUSCHI, J. H.; CAMARGO, L. S. A.; FERNANDES, C. A. C.; BRANDÃO, F. Z. Luteal morphometric parameters and sonographic echotexture during luteogenesis period in pregnant goats. Reproduction, Fertility and Development, v. 20, n. 1, p. 236, 2008. 1 p. Edição dos proceedings of the Annual Conference of the International, Embryo Society, Denver, Colorado, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 72. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; SUDRÉ, A. P.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; MACHADO, M. A.; VIANA, J. H. M. Luteinizing hormone receptor gene expression during follicular divergence in B. taurus vs B. indicus dairy breeds. Animal Reproduction, v.10, n.3, p. 425, Jul./Sept. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 73. | | SIQUEIRA, L. G. B.; TORRES, C. A. A.; VIANA, J. H. M.; SOUZA, E. D.; AMORIM, L. S.; FONSECA, J. F. da; MONTEIRO JÚNIOR, P. L. J.; CAMARGO, L. S. A.; FERNANDES, C. A. C. Uso da análise computacional de imagens de ultra-som na avaliação da ecotextura luteal e sua relação com a concentração de progesterona plasmática durante o ciclo estral de novilhas mestiças (Bos taurus taurus x Bos Taurus indicus). Acta Scientiae Veterinariae, Porto Alegre, v. 35, p. s1301, 2007. Suplemento 3. Edição dos anais da 21o. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões - SBTE, Salvador, 2007.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 74. | | ARASHIRO, E. K. N.; GRAZIA, J. G. V.; SANTOS, J. L. R.; BELMONTE, S. L. R.; PALHÃO, M. P.; FERNANDES, C. A. C.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Use of three-dimensional models as an alternative to evalue body condition in cattle: preliminary results. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 28., 2014, Natal. Proceedings...| Biblioteca(s): Embrapa Gado de Leite. |
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| 75. | | LOPES, F. G.; COSTA, E. P. DA; QUEIROZ-CASTRO, V. L. D.; PEREIRA, E. C. M.; GUIMARÃES, J. D.; ALVES, S. V. P.; FERNANDES, C. A. C.; CAMARGO, L. S. de A.; BENJAMIM, L. DOS A. Use of two new formulations as bovine embryo manipulation solution. Animal Reproduction, v. 16 n. 2, p. 348-355, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 75 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
S. WOHLRES-VIANA, UFJF; E. K. N. ARASHIRO, UFF; T. P. MINARE, Universidade José do Rosario Vellano, Alfenas, MG; C. A. C. FERNANDES, Universidade Jose do Rosario Vellano / Biotran Biotecnologia Animal, Alfenas, MG; J. G. V. GRAZIA, UFMG; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen. |
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Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
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DOI: |
10.1016/j.theriogenology.2018.12.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production. |
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Thesaurus NAL: |
Embryo transfer; Luteinizing hormone. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02857naa a2200253 a 4500
001 2106166
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource]
260 $c2019
520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.
650 $aEmbryo transfer
650 $aLuteinizing hormone
653 $aIn vivo embryo production
700 1 $aARASHIRO, E. K. N.
700 1 $aMINARE, T. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aGRAZIA, J. G. V.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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