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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
09/10/2013 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. A.; ARAGÃO, C. W. S.; CASTRO, M. E. B. de; OLIVEIRA, J. V. de C.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
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Afiliação: |
ANABELE AZEVEDO LIMA, UNB; CLARA WANDENKOLCK SILVA ARAGÃO, UNB; MARIA ELITA BATISTA DE CASTRO, CENARGEN; JULIANA VELASCO DE CASTRO OLIVEIRA, USP; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN MORAIS RIBEIRO, UNB. |
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Título: |
A recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
PLOSone, v. 8, n. 9, e74592, Sept. 2013. |
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DOI: |
10.1371/journal.pone.0074592 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. MenosOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed hi... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90688/1/journal.pone.0074592.pdf
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Marc: |
LEADER 02600naa a2200205 a 4500
001 1976569
005 2023-03-09
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0074592$2DOI
100 1 $aLIMA, A. A.
245 $aA recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity.$h[electronic resource]
260 $c2013
520 $aOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest.
650 $aSoja
700 1 $aARAGÃO, C. W. S.
700 1 $aCASTRO, M. E. B. de
700 1 $aOLIVEIRA, J. V. de C.
700 1 $aSOSA-GÓMEZ, D. R.
700 1 $aRIBEIRO, B. M.
773 $tPLOSone$gv. 8, n. 9, e74592, Sept. 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2008 |
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Data da última atualização: |
25/08/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FARIAS, J. L. de S.; SANTOS, D. O.; FACO, O.; ARAGAO, D. A. de; REGO, J. P. A. do; MEDEIROS, J. N. |
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Afiliação: |
JORGE LUIS DE SALES FARIAS, CNPC; DIÔNES OLIVEIRA SANTOS, CNPC; OLIVARDO FACO, CNPC; DANILO ANDRADE DE ARAGÃO, graduando - Universidade Estadual Vale do Acaraú; JOÃO PAULO ARCELINO DO RÊGO, Pós-graduando Universidade Federal do Ceará; JOSÉ NÓBREGA MEDEIROS, CNPC. |
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Título: |
Comportamento de ovinos jovens da raça Morada Nova aprovados em testes de desempenho à seleção como de doadores de sêmen. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 524. Resumo. |
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Idioma: |
Português |
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Palavras-Chave: |
Banco de sêmen; Raça Morada Nova; Recurso genético animal. |
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Thesagro: |
Comportamento animal; Ovino; Sêmen. |
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Thesaurus NAL: |
Animal genetic resources; Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53870/1/RAC-Comportamento-de-ovinos.pdf
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Marc: |
LEADER 00917nam a2200253 a 4500
001 1534068
005 2021-08-25
008 2008 bl uuuu u00u1 u #d
100 1 $aFARIAS, J. L. de S.
245 $aComportamento de ovinos jovens da raça Morada Nova aprovados em testes de desempenho à seleção como de doadores de sêmen.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 524. Resumo.$c2008
650 $aAnimal genetic resources
650 $aSheep
650 $aComportamento animal
650 $aOvino
650 $aSêmen
653 $aBanco de sêmen
653 $aRaça Morada Nova
653 $aRecurso genético animal
700 1 $aSANTOS, D. O.
700 1 $aFACO, O.
700 1 $aARAGAO, D. A. de
700 1 $aREGO, J. P. A. do
700 1 $aMEDEIROS, J. N.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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