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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
30/09/2021 |
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Data da última atualização: |
30/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, V. N.; GAMA, R. N. C. S.; SANTOS, C. A. F. |
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Afiliação: |
V. N. SANTOS, Universidade Estadual de Feira de Santana; R. N. C. S. C. GAMA, Universidade Estadual de Feira de Santana; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Development and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae: Sapindales), a species endemic to the Brazilian semi-arid region. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Research Society and Development, v. 20, n. 2, May. 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
The umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars. MenosThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), ... Mostrar Tudo |
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Palavras-Chave: |
SSR. |
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Thesagro: |
Melhoramento Genético Vegetal; Spondias Tuberosa; Umbu. |
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Thesaurus Nal: |
Plant breeding; Spondias. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226520/1/Development-and-transferability-of-microsatellite-2021.pdf
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Marc: |
LEADER 02483naa a2200217 a 4500
001 2134937
005 2021-09-30
008 2021 bl uuuu u00u1 u #d
100 1 $aSANTOS, V. N.
245 $aDevelopment and transferability of microsatellite loci for Spondias tuberosa (Anacardiaceae$bSapindales), a species endemic to the Brazilian semi-arid region.$h[electronic resource]
260 $c2021
520 $aThe umbu tree (Spondias tuberosa) is one of the best known plants of the Brazilian semi-arid region; it has great potential for the fruit market due to excellent consumer acceptance. This tree is not presently cultivated; fruit commercialization is based on extrativism. Consequently, there has been little research on the genetics of this species. Our objective was to develop, evaluate and transfer single sequence repeat (SSR) loci to S. tuberosa to support work on genetic resources and agronomic improvement of this species. SSR loci for the umbu tree were developed from a new enriched genomic library and evaluated by PCR. Fourteen SSR loci developed for S. radlkoferi were evaluated for use in S. tuberosa, as well as 18 SSR loci previously identified for this species. DNA was extracted from leaf tissue of eight umbu trees available that are part of a germplasm collection located in Petrolina, PE, Brazil. Of the 14 pairs of primers that were tested, six yielded amplicons, and two showed polymorphism in the genotyped samples. All SSR loci of S. radlkoferi transferred to the umbu tree species, yielding amplicons; however, only four were polymorphic in this sample. Among the eighteen available species-specific SSR loci, six were polymorphic for these eight trees. Among the 38 loci that we evaluated, 12 were polymorphic in the sample, including two new loci for S. tuberosa. The number of alleles ranged from 2 to 12, and 10 of them were moderately to highly informative (PIC>0.50), while nine had heterozygosity greater than 0.50. The six new SSR loci and the 14 SSR loci transferred from S. radlkoferi increase the number of available loci for population studies, germplasm collection and resource management for the development of new umbu tree cultivars.
650 $aPlant breeding
650 $aSpondias
650 $aMelhoramento Genético Vegetal
650 $aSpondias Tuberosa
650 $aUmbu
653 $aSSR
700 1 $aGAMA, R. N. C. S.
700 1 $aSANTOS, C. A. F.
773 $tResearch Society and Development$gv. 20, n. 2, May. 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/04/2015 |
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Data da última atualização: |
12/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RIBEIRO JÚNIOR, G. de O.; VELASCO, F. O.; FARIA JÚNIOR, W. G. de; TEIXEIRA, A. de M.; MACHADO, F. S.; MAGALHÃES, F. A.; JAYME, D. G.; GONÇALVES, L. C. |
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Afiliação: |
G. DE O. RIBEIRO JÚNIOR, UFMG; F. O. VELASCO, UFMG; W. G. DE FARIA JÚNIOR, UFRR; A. DE M. TEIXEIRA, UFU; FERNANDA SAMARINI MACHADO, CNPGL; F. A. MAGALHÃES, UFMG; D. G. JAYME, UFMG; L. C. GONÇALVES, UFMG. |
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Título: |
In situ degradation kinetic of Andropogon gayanus grass silages harvested at three stages of maturity. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 66, n. 6, p. 1883-1890, 2014. |
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DOI: |
https://doi.org/10.1590/1678-6780 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT - Maturity is considered the primary factor affecting the nutritive value of forages. The aim of this study was to evaluate and compare dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation kinetics of Andropogon gayanus grass silages harvested at three stages of maturity (56, 84 and 112 d). Dried and ground silage samples (5 mm) were incubated in nylon bags inside the rumen for 0, 6, 12, 24, 48, 72 and 96 h to estimate the kinetics of ruminal DM, CP and NDF degradation. The ruminal kinetic parameters of the silages from each treatment (56, 84 and 112 d of regrowth) in each animal (5 cows) were determined by the model: y = a + b(1 ? e?c(t?L)). The parameters generated by the model were analyzed as randomized block design, with grass regrowth age as the fixed effect and animal the as random effect (blocks). The grass silage ensiled with 56 days of regrowth had the highest (P<0.05) effective degradability of DM and NDF calculated for fractional rate of particulate passage of 2%/h. All silages had low CP soluble fraction (<25.4%) and effective degradability calculated for fractional rate of particulate passage of 2%/h (<40.1%). A greater (P<0.05) NDF lag time was observed for the silages ensiled with 84 and 112d compared to the silage produced with 56d of regrowth. The ruminal degradation kinetics of Andropogon gayanus grass silages showed that the grass ensiled with 56 days of regrowth had higher nutritional value. RESUMO - Maturity is considered the primary factor affecting the nutritive value of forages. The aim of this study was to evaluate and compare dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation kinetics of Andropogon gayanus grass silages harvested at three stages of maturity (56, 84 and 112 d). Dried and ground silage samples (5 mm) were incubated in nylon bags inside the rumen for 0, 6, 12, 24, 48, 72 and 96 h to estimate the kinetics of ruminal DM, CP and NDF degradation. The ruminal kinetic parameters of the silages from each treatment (56, 84 and 112 d of regrowth) in each animal (5 cows) were determined by the model: y = a + b(1 ? e?c(t?L)). The parameters generated by the model were analyzed as randomized block design, with grass regrowth age as the fixed effect and animal the as random effect (blocks). The grass silage ensiled with 56 days of regrowth had the highest (P<0.05) effective degradability of DM and NDF calculated for fractional rate of particulate passage of 2%/h. All silages had low CP soluble fraction (<25.4%) and effective degradability calculated for fractional rate of particulate passage of 2%/h (<40.1%). A greater (P<0.05) NDF lag time was observed for the silages ensiled with 84 and 112d compared to the silage produced with 56d of regrowth. The ruminal degradation kinetics of Andropogon gayanus grass silages showed that the grass ensiled with 56 days of regrowth had higher nutritional value. MenosABSTRACT - Maturity is considered the primary factor affecting the nutritive value of forages. The aim of this study was to evaluate and compare dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation kinetics of Andropogon gayanus grass silages harvested at three stages of maturity (56, 84 and 112 d). Dried and ground silage samples (5 mm) were incubated in nylon bags inside the rumen for 0, 6, 12, 24, 48, 72 and 96 h to estimate the kinetics of ruminal DM, CP and NDF degradation. The ruminal kinetic parameters of the silages from each treatment (56, 84 and 112 d of regrowth) in each animal (5 cows) were determined by the model: y = a + b(1 ? e?c(t?L)). The parameters generated by the model were analyzed as randomized block design, with grass regrowth age as the fixed effect and animal the as random effect (blocks). The grass silage ensiled with 56 days of regrowth had the highest (P<0.05) effective degradability of DM and NDF calculated for fractional rate of particulate passage of 2%/h. All silages had low CP soluble fraction (<25.4%) and effective degradability calculated for fractional rate of particulate passage of 2%/h (<40.1%). A greater (P<0.05) NDF lag time was observed for the silages ensiled with 84 and 112d compared to the silage produced with 56d of regrowth. The ruminal degradation kinetics of Andropogon gayanus grass silages showed that the grass ensiled with 56 days of regrowth had higher nutritional value. RESUMO - Maturity... Mostrar Tudo |
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Palavras-Chave: |
Gamba grass; Maturity; Preserved forage; Ruminal degradability; Tropical grass. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122515/1/Cnpgl-2014-ArqBrasMedVetZ-In-situ.pdf
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Marc: |
LEADER 03871naa a2200277 a 4500
001 2013820
005 2022-08-12
008 2014 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1678-6780$2DOI
100 1 $aRIBEIRO JÚNIOR, G. de O.
245 $aIn situ degradation kinetic of Andropogon gayanus grass silages harvested at three stages of maturity.$h[electronic resource]
260 $c2014
520 $aABSTRACT - Maturity is considered the primary factor affecting the nutritive value of forages. The aim of this study was to evaluate and compare dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation kinetics of Andropogon gayanus grass silages harvested at three stages of maturity (56, 84 and 112 d). Dried and ground silage samples (5 mm) were incubated in nylon bags inside the rumen for 0, 6, 12, 24, 48, 72 and 96 h to estimate the kinetics of ruminal DM, CP and NDF degradation. The ruminal kinetic parameters of the silages from each treatment (56, 84 and 112 d of regrowth) in each animal (5 cows) were determined by the model: y = a + b(1 ? e?c(t?L)). The parameters generated by the model were analyzed as randomized block design, with grass regrowth age as the fixed effect and animal the as random effect (blocks). The grass silage ensiled with 56 days of regrowth had the highest (P<0.05) effective degradability of DM and NDF calculated for fractional rate of particulate passage of 2%/h. All silages had low CP soluble fraction (<25.4%) and effective degradability calculated for fractional rate of particulate passage of 2%/h (<40.1%). A greater (P<0.05) NDF lag time was observed for the silages ensiled with 84 and 112d compared to the silage produced with 56d of regrowth. The ruminal degradation kinetics of Andropogon gayanus grass silages showed that the grass ensiled with 56 days of regrowth had higher nutritional value. RESUMO - Maturity is considered the primary factor affecting the nutritive value of forages. The aim of this study was to evaluate and compare dry matter (DM), crude protein (CP) and neutral detergent fiber (NDF) ruminal degradation kinetics of Andropogon gayanus grass silages harvested at three stages of maturity (56, 84 and 112 d). Dried and ground silage samples (5 mm) were incubated in nylon bags inside the rumen for 0, 6, 12, 24, 48, 72 and 96 h to estimate the kinetics of ruminal DM, CP and NDF degradation. The ruminal kinetic parameters of the silages from each treatment (56, 84 and 112 d of regrowth) in each animal (5 cows) were determined by the model: y = a + b(1 ? e?c(t?L)). The parameters generated by the model were analyzed as randomized block design, with grass regrowth age as the fixed effect and animal the as random effect (blocks). The grass silage ensiled with 56 days of regrowth had the highest (P<0.05) effective degradability of DM and NDF calculated for fractional rate of particulate passage of 2%/h. All silages had low CP soluble fraction (<25.4%) and effective degradability calculated for fractional rate of particulate passage of 2%/h (<40.1%). A greater (P<0.05) NDF lag time was observed for the silages ensiled with 84 and 112d compared to the silage produced with 56d of regrowth. The ruminal degradation kinetics of Andropogon gayanus grass silages showed that the grass ensiled with 56 days of regrowth had higher nutritional value.
653 $aGamba grass
653 $aMaturity
653 $aPreserved forage
653 $aRuminal degradability
653 $aTropical grass
700 1 $aVELASCO, F. O.
700 1 $aFARIA JÚNIOR, W. G. de
700 1 $aTEIXEIRA, A. de M.
700 1 $aMACHADO, F. S.
700 1 $aMAGALHÃES, F. A.
700 1 $aJAYME, D. G.
700 1 $aGONÇALVES, L. C.
773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia$gv. 66, n. 6, p. 1883-1890, 2014.
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