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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
23/01/2013 |
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Data da última atualização: |
25/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. |
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Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; DIVA CORREIA, CNPAT; WAGENINGEN UNIVERSITY GREENHOUSE HORTICULTURE; PLANT RESEARCH INTERNATIONAL; GILVAN FERREIRA DA SILVA, CPAA; UNIVERSIDADE ESTADUAL DO CEARÁ. |
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Título: |
Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/j.1744-7348.2012.00534.x |
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Idioma: |
Português |
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Conteúdo: |
Members of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity. MenosMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be dete... Mostrar Tudo |
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Palavras-Chave: |
Anacardium occidentale L; Cashew agri-industry; Endophytic fungi; Immunodetection. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03001naa a2200289 a 4500
001 1946100
005 2015-03-25
008 2012 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/j.1744-7348.2012.00534.x$2DOI
100 1 $aMUNIZ, C. R.
245 $aPolyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants.
260 $c2012
520 $aMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity.
653 $aAnacardium occidentale L
653 $aCashew agri-industry
653 $aEndophytic fungi
653 $aImmunodetection
700 1 $aFREIRE, F. das C. O.
700 1 $aVIANA, F. M. P.
700 1 $aCARDOSO, J. E.
700 1 $aCORREIA, D.
700 1 $aJALINK, H.
700 1 $aKEMA, G. H. J.
700 1 $aSILVA, G. F. da
700 1 $aGUEDES, M. I. F.
773 $tAnnals of Applied Biology$gv. 160, n. 3, p. 217-224, 2012.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/12/2023 |
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Data da última atualização: |
13/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
AGUIAR, M. S.; PEREIRA, H. S.; SOUZA, T. L. P. O. de; FARIA, L. C. de; COSTA, J. G. C. da; MAGALDI, M. C. de S.; SOUZA, N. P. de; KNUPP, A. M.; ALMEIDA, V. M. de; MELO, L. C. |
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Afiliação: |
MARCELO SFEIR DE AGUIAR, CNPAF; HELTON SANTOS PEREIRA, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; LUIS CLAUDIO DE FARIA, CNPAF; JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; MARIANA CRUZICK DE S MAGALDI, CNPAF; NILDA PESSOA DE SOUZA, CNPAF; ADRIANO MOREIRA KNUPP, CNPAF; VALTER MARTINS DE ALMEIDA, EMPAER-MT; LEONARDO CUNHA MELO, CNPAF. |
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Título: |
BRS FS313: Jalo type common bean cultivar with large beans and high yield. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Functional Plant Breeding Journal, v. 5, n. 2, p. 13-19, Jan./Dec. 2023. |
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ISSN: |
2595-9433 |
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DOI: |
http://dx.doi.org/10.35418/2526-4117/v5a2 |
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Idioma: |
Inglês |
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Conteúdo: |
BRS FS313 is a common bean cultivar with high yield (3,200 kg.ha-1), jalo type bean grain, and greater 100-seed weight (50 g). It has a semi-early cycle, semi-up-right plant architecture, and resistance to anthracnose and to root rots. BRS FS313 is recommended for the Central and South-Central regions of Brazil. |
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Palavras-Chave: |
BRS FS313. |
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Thesagro: |
Antracnose; Feijão; Melhoramento Genético Vegetal; Phaseolus Vulgaris; Produtividade; Variedade Resistente. |
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Thesaurus NAL: |
Beans; New variety; Plant breeding. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159621/1/fpbj-2023.pdf
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Marc: |
LEADER 01398naa a2200373 a 4500
001 2159621
005 2023-12-13
008 2023 bl uuuu u00u1 u #d
022 $a2595-9433
024 7 $ahttp://dx.doi.org/10.35418/2526-4117/v5a2$2DOI
100 1 $aAGUIAR, M. S.
245 $aBRS FS313$bJalo type common bean cultivar with large beans and high yield.$h[electronic resource]
260 $c2023
520 $aBRS FS313 is a common bean cultivar with high yield (3,200 kg.ha-1), jalo type bean grain, and greater 100-seed weight (50 g). It has a semi-early cycle, semi-up-right plant architecture, and resistance to anthracnose and to root rots. BRS FS313 is recommended for the Central and South-Central regions of Brazil.
650 $aBeans
650 $aNew variety
650 $aPlant breeding
650 $aAntracnose
650 $aFeijão
650 $aMelhoramento Genético Vegetal
650 $aPhaseolus Vulgaris
650 $aProdutividade
650 $aVariedade Resistente
653 $aBRS FS313
700 1 $aPEREIRA, H. S.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, L. C. de
700 1 $aCOSTA, J. G. C. da
700 1 $aMAGALDI, M. C. de S.
700 1 $aSOUZA, N. P. de
700 1 $aKNUPP, A. M.
700 1 $aALMEIDA, V. M. de
700 1 $aMELO, L. C.
773 $tFunctional Plant Breeding Journal$gv. 5, n. 2, p. 13-19, Jan./Dec. 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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