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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/12/2023 |
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Data da última atualização: |
11/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, J. R. da; SILVA, J. F. M.; PEREIRA, M. F.; TORRES, A. R.; GONÇALVES, M. S.; PRATA, M. C. de A.; BRITO, M. A. V. P. e; COSTA, G. M. da; RIBEIRO, J. B. |
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Afiliação: |
JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL. |
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Título: |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. |
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DOI: |
https://doi.org/10.1007/s42770-023-01181-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. MenosStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic tar... Mostrar Tudo |
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Palavras-Chave: |
Infecção intramamária; Modelo de invertebrados; Virulência bacteriana. |
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Thesagro: |
Bovino; Doença Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/262967/1/Is-Galleria-mellonella-model-a-good-alternative-to-study-virulence.pdf
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Marc: |
LEADER 02652naa a2200289 a 4500
001 2159710
005 2024-03-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-023-01181-1$2DOI
100 1 $aSILVA, J. R. da
245 $aIs Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?$h[electronic resource]
260 $c2024
520 $aStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.
650 $aBovino
650 $aDoença Animal
653 $aInfecção intramamária
653 $aModelo de invertebrados
653 $aVirulência bacteriana
700 1 $aSILVA, J. F. M.
700 1 $aPEREIRA, M. F.
700 1 $aTORRES, A. R.
700 1 $aGONÇALVES, M. S.
700 1 $aPRATA, M. C. de A.
700 1 $aBRITO, M. A. V. P. e
700 1 $aCOSTA, G. M. da
700 1 $aRIBEIRO, J. B.
773 $tBrazilian Journal of Microbiology$gv. 55, n. 1, p. 889-900, 2024.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 283 | |
| 2. |  | FAJARDO, T. V. M. Controle de doenças causadas por vírus. In: GARRIDO, L. da R.; HOFFMANN, A.; BOTTON, M. (Ed.). Boas práticas agrícolas na viticultura: manejo de pragas e doenças. Brasília, DF: SEBRAE; Bento Gonçalves: Embrapa Uva e Vinho: IBRAVIN, 2011. p. 53-74. (Boas Práticas Agrícolas - BPA; Uva para processamento, 4). Programa de Desenvolvimento da Cadeia Produtiva de Vinhos, Espumantes e Suco de Uva - PAS.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 3. | | FAJARDO, T. V. M. Controle de doenças causadas por vírus. IN: GARRIDO, L. da R.; HOFFMANN, A.; SILVEIRA, S. V. da. (Ed.). Produção integrada de uva para processamento: manejo de pragas e doenças. Brasília, DF: Embrapa, 2015. v. 4, cap. 3, p. 49-70.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | FAJARDO, T. V. M. Controle de doenças causadas por vírus. In: HOFFMANN, A.; SILVEIRA, S. V.; GARRIDO, L.R. (Eds.). Manual Técnico de Boas Práticas Agrícolas - Uva para Processamento. Brasília, DF: SEBRAE; Bento Gonçalves, RS: IBRAVIN, 2016. pp. 151-171.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 5. | | FAJARDO, T. V. M. Controle de doenças causador por vírus. IN: GARRIDO, L. da R.; HOFFMANN, A.; SILVEIRA, S. V. da (Ed.). Produção integrada de uva para processamento: manejo de pragas e doenças. Brasília, DF: Embrapa, 2015. v. 4, cap. 3, p. 49-70.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | FAJARDO, T. V. M. Vírus nas mudas. A Vindima, Flores da Cunha, v. 4, n. 30, p. 14, jun. 2011.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 11. | | FAJARDO, T. V. M. (ed.). Uva para processamento: fitossanidade. Brasília, DF: Embrapa Informação Tecnológica; Bento Gonçalves: Embrapa Uva e Vinho, 2003. 128 p. il. (Frutas do Brasil, 35). Inclui glossário.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| 15. | | NICKEL, O.; FAJARDO, T. V. M. Detection of four viruses in apples and pears by real time RT-PCR using 5'-hydrolysis probes. Journal of the Brazilian Society for Virology, Novo Hamburgo, v. 18, p. 28-29, 2013. Resumo apresentado no XXIV Brazilian Congress of Virology & VIII Mercosur Meeting of Virology, September, 1 - 4, 2013 - Porto Seguro, Bahia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 19. | | KUHN, G. B.; FAJARDO, T. V. M. Doenças de uva. Cultivar Hortaliças e Frutas, Pelotas, v. 1, n. 1, p. 23-27, abr./maio 2000. p. 23-27 il.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 20. | | FAJARDO, T. V. M.; EIRAS, M. Doenças virais em videiras. In:BUENO, C. J. (Org.). Doenças e pragas em videiras. São Paulo, SP: Instituto Biológico de São Paulo, nov. 2022. (online). (Boletim Técnico, 33). pp. 61-103.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 283 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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