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Registros recuperados : 174 | |
123. | | OLIVEIRA, R. S. de; SOUZA, S. de O.; AONA, L. Y. S.; SOUZA, F. V. D.; ROSSI, M. L.; SOUZA, E. H. de. Leaf structure of Tillandsia species (Tillandsioideae: Bromeliaceae) by light microscopy and scanning electron microscopy. Microsc Res Tech, v.85, n.1, p.253-269. Jan, 2022. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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124. | | SOARES, T. L.; SANTOS-SEREJO, J. A.; COSTA, M. A. P. de C.; SOUZA, E. H. de; SOUZA, A. da S. Influência do momento da coleta sobre a viabilidade de grãos de pólen de bananeira Musa acuminata. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 2507 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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126. | | SOUZA, E. H. de; MATOS, A. P. de; SOUZA, F. V. D.; COSTA JÚNIOR, D. S.; TROCOLI, R. O.; COSTA, M. A. P. de C. Evaluation of ornamental pineapple hybrids for resistance to Fusarium subglutinans f. sp. ananas. Acta Horticulturae, Johor Bahru, Malaysia, v. 902, p. 381-385, 2011. 0567-7572 VII International Pineapple Symposium. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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127. | | SOUZA, E. H. de; TROCOLI, R. O.; MATOS, A. P. de; COSTA JÚNIOR, D. S.; SOUZA, F. V. D.; COSTA, M. A. P. de C. Evaluation of ornamental pineapple hybrids for resistance to Fusarium subglutinans. Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. Disponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011. Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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128. | | SOUZA, F. V. D.; CABRAL, J. R. S.; SOUZA, E. H. de; SILVA, M. de J.; SANTOS, O. S. N.; FERREIRA, F. R. Evaluation of F1 hybrids between Ananas comosus var. ananassoides and Ananas comosus var. erectifolius. Acta Horticulturae, Leuven, n. 822, p. 79-84, mar. 2009. Edição de Proceedings of the VI International Pineapple Symposium, João Pessoa, nov. 2007. Disponível também em CD-ROM e on-line. Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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129. | | SOARES, T. L.; SOUZA, E. H. de; SOUZA, F. V. D.; SOUZA, A. da S.; SANTOS-SEREJO, J. A. dos; COSTA, M. A. P. de C. Germinação in vitro e viabilidade de grãos de pólen em diferentes acessos de abacaxi ornamental. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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130. | | LINS, L. C. R. de; SILVA, S. de O. e; SOARES, T. L.; COSTA, M. A. P. de C.; SOUZA, E. H. de; SANTOS-SEREJO, J. A. dos. Germinação in vitro e viabilidade de pólen de genótipos tetraplóides de bananeira. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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132. | | SOARES, T. L.; SOUZA, E. H. de; SOUZA, F. V. D.; SOUZA, A. da S.; SANTOS-SEREJO, J. A. dos; COSTA, M. A. P. de C. Efeito da temperatura na germinação in vitro de grãos de pólen de abacaxi ornamental. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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133. | | SOARES, T. L.; SOUZA, E. H. de; SAMPAIO, L. F. S.; COSTA, M. A. P. de C.; SILVA, S. de O. e; SEREJO, J. A. dos S. Effect of collection time on the viability of banana pollen grains. African Journal of Biotechnology, v.14, n. 4, p.1207-1214, April, 2015. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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134. | | BASTOS, M. J. S. M.; BASTOS, L. P.; SOUZA, E. H. de; SOARES, T. L.; MORAIS, D. V.; SOUZA, F. V. D.; COSTA, M. A. P. de C. Floral and reproductive biology of Alcantarea nahoumii (Bromeliaceae),a vulnerable endemic species of the Atlantic Forest. Acta Botanica Brasilica, v31, n.4, p. 665-676, October/December, 2017. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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136. | | SOUZA, E. H. de; SOUZA, F. V. D.; SILVA, M. de J. da; SOUZA, A. da S.; COSTA, M. A. P. de C. Growth regulators and physical state of culture media in the micropropagation of ornamental pineapple hybrids. Plant Cell Culture & Micropropagation, Lavras, v.8, n. 1-2, p. 10-17, 2012. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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137. | | SOUZA, E. H. de; SOUZA, F. V. D.; SANTOS-SEREJO, J. A. dos; SOARES, T. L.; SILVA, S. de O. e; COSTA, M. A. P. de C. Inflorescências masculinas de bananeira com potencial ornamental. In: ENCONTRO DA REDE DE RECURSOS GENÉTICOS VEGETAIS DO ESTADO DA BAHIA, 3.; SIMPÓSIO DE RECURSOS GENÉTICOS DE PLANTAS CULTIVADAS NO NORDESTE BRASILEIRO, 2., 2008, Vitória da Conquista. [Anais]... Vitória da Conquista: Universidade Estadual do Sudoeste da Bahia, 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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138. | | SILVA, R. S.; ALVES, B. L.; SOUZA, F. V. D.; SOUZA, F. V. D.; SOUZA, E. H. de; SOARES FILHO, W. dos S. Obtenção de híbridos de citros para fins ornamentais In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p. 1p. Desenvolvimento de Variedades Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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139. | | MUNIZ, N. P.; GONÇALVES, J. S.; SILVA, H. S. A.; SOUZA, E. H. de; COELHO FILHO, M. A.; SOUZA, F. V. D. Pré-aclimatização de mudas de abacaxizeiro 'Imperial' em substrato suplementado com rizobactérias. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 9., 2015: Cruz das Almas, BA. Pesquisa: para quê? para quem? : resumos. Brasília, DF : Embrapa, 2015. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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140. | | SOUZA, E. H. de; MASSARIOLI, A.; MORENO, I. A. M.; ALENCAR, S. M. de; SOUZA, F. V. D.; MARTINELLI, A. P. Quantificação e composição do néctar em bromeliaceae. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 174 | |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
08/06/2018 |
Data da última atualização: |
29/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
Idioma: |
Português |
Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
Thesaurus NAL: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
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Marc: |
LEADER 02824naa a2200277 a 4500 001 2092380 005 2019-04-29 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI 100 1 $aBOSCO, D. D. 245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource] 260 $c2018 520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis 650 $aChitinase 650 $aEpigenetics 650 $aGrapevine leafroll-associated virus 3 650 $aVitis 653 $aFungus 653 $aPathogenesis related protein 5 700 1 $aSINSKI, I. 700 1 $aRITSCHEL, P. S. 700 1 $aCAMARGO, U. A. 700 1 $aFAJARDO, T. V. M. 700 1 $aHARAKAVA, R. 700 1 $aQUECINI, V. 773 $tTransgenic Research, p. 1-18, 2018. [Online]
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