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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
21/11/2012 |
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Data da última atualização: |
14/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
D'AFONSECA, V.; SOARES, S. C.; ALI, A.; SANTOS, A. R.; PINTO, A. C.; MAGALHÃES, A. A. C.; FARIA, C. de J.; BARBOSA, E.; GUIMARÃES, L. C.; ESLABÃO, M.; ALMEIDA, S. S.; ABREU, V. A. C.; ZERLOTINI, A.; CARNEIRO, A. R.; CERDEIRA, L. T.; RAMOS, R. T. J.; HIRATA JÚNIOR, R.; MATTOS-GUARALDI, A. L.; TROST, E.; TAUCH, A.; SILVA, A.; SCHNEIDER, M. P.; MIYOSHI, A.; AZEVEDO, V. |
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Afiliação: |
VÍVIAN D’AFONSECA, UFMG; SIOMAR C. SOARES, UFMG; AMJAD ALI, UFMG; ANDERSON R. SANTOS, UFMG; ANNE C. PINTO, UFMG; ARYANE A. C. MAGALHÃES, UFMG; CÁSSIO DE JESUS FARIA, UFMG; EUDES BARBOSA, UFMG; LUIS C. GUIMARÃES, UFMG; MARCUS ESLABÃO, UFPel; SINTIA S. ALMEIDA, UFMG; VINICIUS A. C. ABREU, UFMG; ADHEMAR ZERLOTINI NETO, CNPTIA; ADRIANA R. CARNEIRO, UFPA; LOUISE T. CERDEIRA, UFPA; ROMMEL T. J. RAMOS, UFPA; RAPHAEL HIRATA JÚNIOR, UERJ; ANA L. MATTOS-GUARALDI, UERJ; EVA TROST, Bielefeld University; ANDREAS TAUCH, Bielefeld University; ARTUR SILVA, UFPA; MARIA P. SCHNEIDER, UFPA; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG. |
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Título: |
Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Open Access Bioinformatics, v. 4, p. 1-13, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. MenosBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already des... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática. |
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Thesagro: |
Genoma; Patogenicidade. |
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Thesaurus Nal: |
Bioinformatics; Corynebacterium diphtheriae; Genome; Pathogenicity islands. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70533/1/OAB-25500-re-annotation-of-the-corynebacterium-diphtheriae-nctc13129-g-022412.pdf
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Marc: |
LEADER 03832naa a2200481 a 4500
001 1940203
005 2020-04-14
008 2012 bl uuuu u00u1 u #d
100 1 $aD'AFONSECA, V.
245 $aReannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria.$h[electronic resource]
260 $c2012
520 $aBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl.
650 $aBioinformatics
650 $aCorynebacterium diphtheriae
650 $aGenome
650 $aPathogenicity islands
650 $aGenoma
650 $aPatogenicidade
653 $aBioinformática
700 1 $aSOARES, S. C.
700 1 $aALI, A.
700 1 $aSANTOS, A. R.
700 1 $aPINTO, A. C.
700 1 $aMAGALHÃES, A. A. C.
700 1 $aFARIA, C. de J.
700 1 $aBARBOSA, E.
700 1 $aGUIMARÃES, L. C.
700 1 $aESLABÃO, M.
700 1 $aALMEIDA, S. S.
700 1 $aABREU, V. A. C.
700 1 $aZERLOTINI, A.
700 1 $aCARNEIRO, A. R.
700 1 $aCERDEIRA, L. T.
700 1 $aRAMOS, R. T. J.
700 1 $aHIRATA JÚNIOR, R.
700 1 $aMATTOS-GUARALDI, A. L.
700 1 $aTROST, E.
700 1 $aTAUCH, A.
700 1 $aSILVA, A.
700 1 $aSCHNEIDER, M. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
773 $tOpen Access Bioinformatics$gv. 4, p. 1-13, 2012.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
08/01/2021 |
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Data da última atualização: |
08/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
OLIVEIRA, V. da S.; MORAIS, J. A. da S.; FAGUNDES, J. L.; SANTANA, J. C. S.; MUNIZ, E. N.; VALENCA, R. de L. |
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Afiliação: |
VINICIUS DA SILVA OLIVEIRA; JUCILEIA APARECIDA DA SILVA MORAIS; JAILSON LARA FAGUNDES; JULIANA CAROLINA SANTOS SANTANA; EVANDRO NEVES MUNIZ, CPATC; ROBERTA DE LIMA VALENCA. |
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Título: |
Fracionamento de proteína e carboidratos segundo CNCPS de cinco forrageiras irrigadas ou não durante a seca. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Research, Society and Development, v. 9, n. 7, e77973582, 2020. |
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ISSN: |
2525-3409 |
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DOI: |
http://dx.doi.org/10.33448/rsd-v9i7.3582 |
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Idioma: |
Inglês |
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Thesagro: |
Andropogon Gayanus; Capim Urochloa; Carboidrato; Forragem; Irrigação; Panicum Maximum; Proteína. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220042/1/CNCPS-de-cinco-forrageiras-2020-3582-16208-1-PB.pdf
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Marc: |
LEADER 00866naa a2200277 a 4500
001 2129117
005 2021-01-08
008 2020 bl uuuu u00u1 u #d
022 $a2525-3409
024 7 $ahttp://dx.doi.org/10.33448/rsd-v9i7.3582$2DOI
100 1 $aOLIVEIRA, V. da S.
245 $aFracionamento de proteína e carboidratos segundo CNCPS de cinco forrageiras irrigadas ou não durante a seca.$h[electronic resource]
260 $c2020
650 $aAndropogon Gayanus
650 $aCapim Urochloa
650 $aCarboidrato
650 $aForragem
650 $aIrrigação
650 $aPanicum Maximum
650 $aProteína
700 1 $aMORAIS, J. A. da S.
700 1 $aFAGUNDES, J. L.
700 1 $aSANTANA, J. C. S.
700 1 $aMUNIZ, E. N.
700 1 $aVALENCA, R. de L.
773 $tResearch, Society and Development$gv. 9, n. 7, e77973582, 2020.
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Embrapa Tabuleiros Costeiros (CPATC) |
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