Data/hora: 09/06/2024 14:45:21

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 19/10/2020
Data da última atualização: | 19/10/2020
Autoria: | SANTOS, V. M. B. dos; PINTO, P. H. N.; BALARO, M. F. A.; SANTOS, J. D. R.; TAIRA, A. R.; ESPIRITO SANTO, C. G. do; GONÇALVES, F. M.; FONSECA, J. F. da; BRANDÃO, F. Z.
Afiliação: | VANESSA M. B. DOS SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; PEDRO HENRIQUE N. PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MARIO FELIPE A. BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JULIANA D. R. SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; AUGUSTO R. TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINE G. DO ESPIRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FERNANDA M. GONÇALVES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE Z. BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.
Título: | Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep.
Ano de publicação: | 2020
Fonte/Imprenta: | Reproduction in Domestic Animals, v. 55, n. 10, p. 1446-1454, Oct. 2020.
DOI: | https://doi.org/10.1111/rda.13795
Idioma: | Inglês
Conteúdo: | Abstract: The aim of this work was to determine whether a cervical dilation protocol (CDP) composed of only oxytocin can be used to perform transcervical (non?surgical) embryo transfer in sheep (NSET) without affecting the viability of the corpus luteum (CL). Likewise, we evaluated whether a cervical transposing test with a Hegar dilator (CT Hegar test), performed at oestrous time, could be used to screen ewes for NSET (greater or lower chances to transpose the cervix). For that, oestrous and ovulation synchronization was performed in 25 Santa Inês ewes to induce the dioestrous condition. Animals went through the following CDP in a crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) and oxytocin (100 IU IV); OX, oxytocin (100 IU IV); and SAL, saline solution (IV). Using a Hegar dilator, cervical transposing attempts were performed at oestrous (D0) and dioestrous time (D8). The viability of the CL (morphology, luteal blood flow and progesterone values) was evaluated by ultrasonography (colour Doppler and B?mode) and by serum progesterone measurement from D7 to D13. The cervical transposing rate was lower for the SAL group (64%; 16/25; p < .05) and did not differ between the E + OX (88%; 22/25, p > .05) and OX (84%; 21/25, p > .05) groups. No treatment affected the CL viability. The CT Hegar test showed a high sensitivity (85.7%?93.3%), satisfactory accuracy (72%?84%), low false?negative rate (6.7%?14.6%), but high false?positive rate (46%?66.7%). In conclusion, a CDP protocol composed exclusively of oxytocin can lead to good cervical transposing rates and does not affect the viability of the CL. In addition, a screening test (CT Hegar) performed at oestrus can identify ewes for which cervical transposing will likely not occur at NSET.
Palavras-Chave: | Dilatation; Luteal phase.
Thesagro: | Ovelha; Ovino; Reprodução Animal; Transferência de Embrião.
Thesaurus Nal: | Animal reproduction; Embryo transfer; Oxytocin; Sheep.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (39654-1) - UPC - AP - DD -  -  -  - DIS