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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
05/12/2016 |
Data da última atualização: |
05/12/2016 |
Autoria: |
FLÓREZ, L. M. M.; BALLESTERO, H. F.; DUZANSKI, A. P.; BERSANO, P. R. O.; LIMA, J. F.; CRUZ, F. L.; MOTA, L. S.; ROCHA, N. S. |
Afiliação: |
LUIS M. M. FLÓREZ, UNESP/Universidade de Caldas; HALINE F. BALLESTERO, UNESP; ANDERSON P. DUZANSKI, UNESP; PAULO R. O. BERSANO, UECE; JOÃO F. LIMA, CEVAP/UNESP; FERNANDA L. CRUZ, UNESP; LÍGIA S. MOTA, UNESP; NOEME S. ROCHA, UNESP. |
Título: |
Immunocytochemical characterization of primary cell culture in canine transmissible venereal tumor. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 39, n. 9, p. 844-850, set. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Immunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. |
Palavras-Chave: |
Alfa 1 antitripsina; Alpha 1 antitrypsin; Canine transmissible venereal tumo; Isozima; Tumor venéreo transmissível canino; Vimentina. |
Thesagro: |
Citogenética. |
Thesaurus Nal: |
Cytogenetics; Lysozyme; Vimentin. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151255/1/Immunocytochemical-characterization.pdf
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Marc: |
LEADER 02414naa a2200325 a 4500 001 2057977 005 2016-12-05 008 2016 bl uuuu u00u1 u #d 100 1 $aFLÓREZ, L. M. M. 245 $aImmunocytochemical characterization of primary cell culture in canine transmissible venereal tumor. 260 $c2016 520 $aImmunochemistry with anti-vimentin, anti-lysozyme, anti-alpha 1 antitrypsin, anti-CD3 and anti-CD79α antibodies has been used for characterization of primary cell culture in the transmissible venereal tumor (TVT). Samples for primary cell culture and immunohistochemistry assays were taken from eight dogs with cytological and clinical diagnosis of TVT. To validate the immunochemical results in the primary cell culture of TVT, a chromosome count was performed. For the statistical analysis, the Mann-Whitney test with p<0.05 was used. TVT tissues and culture cells showed intense anti-vimentin immunoreactivity, lightly to moderate immunoreactivity for anti-lysozyme, and mild for anti-alpha-antitrypsin. No marking was achieved for CD3 and CD79α. All culture cells showed chromosomes variable number of 56 to 68. This is the first report on the use of immunocytochemical characterization in cell culture of TVT. Significant statistic difference between immunochemistry in tissue and culture cell was not established, what suggests that the use of this technique may provide greater certainty for the confirmation of tumors in the primary culture. This fact is particularly important because in vitro culture of tumor tissues has been increasingly used to provide quick access to drug efficacy and presents relevant information to identify potential response to anticancer medicine; so it is possible to understand the behavior of the tumor. 650 $aCytogenetics 650 $aLysozyme 650 $aVimentin 650 $aCitogenética 653 $aAlfa 1 antitripsina 653 $aAlpha 1 antitrypsin 653 $aCanine transmissible venereal tumo 653 $aIsozima 653 $aTumor venéreo transmissível canino 653 $aVimentina 700 1 $aBALLESTERO, H. F. 700 1 $aDUZANSKI, A. P. 700 1 $aBERSANO, P. R. O. 700 1 $aLIMA, J. F. 700 1 $aCRUZ, F. L. 700 1 $aMOTA, L. S. 700 1 $aROCHA, N. S. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 39, n. 9, p. 844-850, set. 2016.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
02/09/1997 |
Data da última atualização: |
08/04/2019 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ELOY, A. M. X.; SANTA ROSA, J. |
Afiliação: |
ANGELA MARIA XAVIER ELOY, CNPC; Janete Santa Rosa, CNPC. |
Título: |
Perfis plasmáticos de testosterona durante a puberdade de machos caprinos da raca Moxotó. |
Ano de publicação: |
1996 |
Fonte/Imprenta: |
In: EMBRAPA. Centro Nacional de Pesquisa de Caprinos. Relatório Técnico do Centro de Pesquisa de Caprinos 1987-1995. Sobral: EMBRAPA-CNPC, 1996. p. 89-91. |
Idioma: |
Português |
Conteúdo: |
Pretendeu-se neste trabalho, estudar as relacoes entre sistema endocrino, mensuracoes testiculares, comportamento sexual e histologia, procurandoo desta forma conhecer o processo de desencadeamento reprodutivo do macho caprino. |
Palavras-Chave: |
Animal histology; Correlação genética; Desbridamento do pênis; Penis detachment; Perímetro escrotal; Puberdade; Raça Moxotó; Scrotal perimeter; Sexual behaviour; Testicular histology. |
Thesagro: |
Caprino; Comportamento sexual; Endocrinologia; Histologia animal; Reprodução animal; Testículo; Testosterona. |
Thesaurus NAL: |
Genetic correlation; Goats; puberty; Sexual maturity; Testes; Testosterone. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/124554/1/CNPC-1996-Perfis-de.pdf
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Marc: |
LEADER 01440nam a2200397 a 4500 001 1514723 005 2019-04-08 008 1996 bl uuuu u00u1 u #d 100 1 $aELOY, A. M. X. 245 $aPerfis plasmáticos de testosterona durante a puberdade de machos caprinos da raca Moxotó. 260 $aIn: EMBRAPA. Centro Nacional de Pesquisa de Caprinos. Relatório Técnico do Centro de Pesquisa de Caprinos 1987-1995. Sobral: EMBRAPA-CNPC, 1996. p. 89-91.$c1996 520 $aPretendeu-se neste trabalho, estudar as relacoes entre sistema endocrino, mensuracoes testiculares, comportamento sexual e histologia, procurandoo desta forma conhecer o processo de desencadeamento reprodutivo do macho caprino. 650 $aGenetic correlation 650 $aGoats 650 $apuberty 650 $aSexual maturity 650 $aTestes 650 $aTestosterone 650 $aCaprino 650 $aComportamento sexual 650 $aEndocrinologia 650 $aHistologia animal 650 $aReprodução animal 650 $aTestículo 650 $aTestosterona 653 $aAnimal histology 653 $aCorrelação genética 653 $aDesbridamento do pênis 653 $aPenis detachment 653 $aPerímetro escrotal 653 $aPuberdade 653 $aRaça Moxotó 653 $aScrotal perimeter 653 $aSexual behaviour 653 $aTesticular histology 700 1 $aSANTA ROSA, J.
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