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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
01/03/2010 |
Data da última atualização: |
11/04/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
JESUS, E. M. de; SALIS, S. M. de; REIS, V. D. A. dos; CASTRO, W. J. P.; ALMEIDA, A. M. de; AZEVEDO, D. T. de; DOMINGOS BRANCO, O. |
Afiliação: |
Edileuza Medeiros de Jesus, Uniderp; SUZANA MARIA DE SALIS, CPAP; VANDERLEI DONISETI ACASSIO DOS REIS, CPAP; Wendy July Padilha Castro, UFMS; Ademir Marques de Almeida, UFMS; Damião Teixeira de Azevedo, UFMS; OSLAIN DOMINGOS BRANCO, CPAP. |
Título: |
Calendário floral para a região do Maciço do Urucum, MS. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL E UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL, 3., SEMANA DE BIOLOGIA, 8. 2009, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2009. (Embrapa Pantanal. Documentos, 101). |
Páginas: |
p. 22 |
Idioma: |
Português |
Palavras-Chave: |
Calendario floral. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00757naa a2200205 a 4500 001 1791002 005 2019-04-11 008 2009 bl --- 0-- u #d 100 1 $aJESUS, E. M. de 245 $aCalendário floral para a região do Maciço do Urucum, MS. 260 $c2009 300 $ap. 22 653 $aCalendario floral 700 1 $aSALIS, S. M. de 700 1 $aREIS, V. D. A. dos 700 1 $aCASTRO, W. J. P. 700 1 $aALMEIDA, A. M. de 700 1 $aAZEVEDO, D. T. de 700 1 $aDOMINGOS BRANCO, O. 773 $tIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL E UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL, 3., SEMANA DE BIOLOGIA, 8. 2009, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2009. (Embrapa Pantanal. Documentos, 101).
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
04/03/2011 |
Data da última atualização: |
04/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SATO, J. H.; TEIXEIRA, J. B.; EIRA, M. T. S. da; FRANCO, O. L.; REIS, A. M. dos. |
Afiliação: |
UNB; JOAO BATISTA TEIXEIRA, CENARGEN; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, CENARGEN. |
Título: |
Comparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
Idioma: |
Inglês |
Conteúdo: |
Coffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee. |
Palavras-Chave: |
Coffee; In vitro micropropagation. |
Thesaurus NAL: |
Somatic embryogenesis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02078nam a2200193 a 4500 001 1880074 005 2011-03-04 008 2008 bl uuuu u00u1 u #d 100 1 $aSATO, J. H. 245 $aComparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008 520 $aCoffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee. 650 $aSomatic embryogenesis 653 $aCoffee 653 $aIn vitro micropropagation 700 1 $aTEIXEIRA, J. B. 700 1 $aEIRA, M. T. S. da 700 1 $aFRANCO, O. L. 700 1 $aREIS, A. M. dos
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