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| Registros recuperados : 109 | |
| 28. |  | SATO, J. H.; TEIXEIRA, J. B.; EIRA, M. T. S.; FRANCO, O. L.; MEHTA, A. Proteômica comparativa em embriões somáticos de Coffea arabica nas diferentes fases da embriogênese. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 050. p. 90.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | FRANCA NETO, J. de B.; HENNING, A. A.; EIRA, M. T. S. Implantacao de testes de sanidade como rotina em laboratorios de analise de sementes. In: SIMPOSIO BRASILEIRO DE PATOLOGIA DE SEMENTES, 1, 1984, Piracicaba, SP. Situacao e perspectivas da patologia de sementes no Brasil; Anais. Piracicaba, CENA/USP-CEN, Brasilia, ABRATES, 1984. p.107-111.| Biblioteca(s): Embrapa Soja. |
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| 33. | | MEHTA, A.; ANDRADE, A. E.; OLIVEIRA, A. C.; RIBEIRO, F. N. S.; EIRA, M. T. S. Expressão diferencial de proteínas em sementes de Coffea arabica submetidas ao estresse hídrico. In: CONGRESSO BRASILEIRO DE GENÉTICA, 50., 2004, Costão do Santinho, Florianópolis. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2004. p. 1166.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 35. | | CUNHA, R.; SALOMAO, A. N.; EIRA, M. T. S.; FAIAD, M. G. R.; GOEDERT, C. O. Seed storage behaviour of Brazilian forest species. In: INTERNATIONAL WORKSHOP ON SEEDS, 5., 1995, Reading, UK. Abstracts of poster presentations. Reading: The University of Reading, 1995. nao paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registro Completo
|
Biblioteca(s): |
Embrapa Café. |
|
Data corrente: |
13/04/2011 |
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Data da última atualização: |
13/04/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
EIRA, M. T. S. da; RIBEIRO, V. S.; FUZUOLI, L. C.; GUERREIRO FILHO, O.; WALTERS, C. |
|
Afiliação: |
MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; IAC; IAC; NCGRP/USDA. |
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Título: |
Coffee seed cryopreservation: current research progress. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Viability of seeds stored in genebanks must be maintained for several years or even centuries. Because of the difficulties in storing the seeds, coffee germplasm is maintained in field collections, presenting significant problems, such as land and labor costs and susceptibility to environmental hazards and pathogens. Storage of Coffea species in ex situ genebanks may help to preserve the threatened diversity of this important genus. Since 1976 the International Plant Genetic Resources Institute has considered coffee as a high priority for genetic conservation. For non-orthodox seed species, cryopreservation is the only technique available for long-term germplasm conservation. The genebank of Embrapa Genetic Resources and Biotechnology, in Brazil has now established a program to cryopreserve genetic resources of Coffea. The protocol was first determined for C. arabica and C. racemosa. Seeds were first dried to 0.20 g/g (in equilibrium with 78-80% RH). Sufficiently rapid cooling and warming was achieved in hermetically-sealed foil-laminate bags containing 10-11 g (or 50 seeds) by plunging bags directly into liquid nitrogen ? LN containers and placing bags removed from LN directly into a 40 °C bath. Successfully cryopreserved C. arabica and C. racemosa seeds showed minimal viability loss after two-year storage in liquid nitrogen. The same protocol is being adapted to other species of Coffea. |
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Palavras-Chave: |
Coffee germplasm; Genebanks. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32596/1/Coffee-Seed-Cryopreservation.pdf
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Marc: |
LEADER 02076nam a2200181 a 4500
001 1885759
005 2011-04-13
008 2007 bl uuuu u00u1 u #d
100 1 $aEIRA, M. T. S. da
245 $aCoffee seed cryopreservation$bcurrent research progress.$h[electronic resource]
260 $aIn:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 21., 2006, Montpellier, France. Table of contents... Montpellier, France: Association for Science and Information on Coffee, 2007. 1 CD-ROM.$c2007
520 $aViability of seeds stored in genebanks must be maintained for several years or even centuries. Because of the difficulties in storing the seeds, coffee germplasm is maintained in field collections, presenting significant problems, such as land and labor costs and susceptibility to environmental hazards and pathogens. Storage of Coffea species in ex situ genebanks may help to preserve the threatened diversity of this important genus. Since 1976 the International Plant Genetic Resources Institute has considered coffee as a high priority for genetic conservation. For non-orthodox seed species, cryopreservation is the only technique available for long-term germplasm conservation. The genebank of Embrapa Genetic Resources and Biotechnology, in Brazil has now established a program to cryopreserve genetic resources of Coffea. The protocol was first determined for C. arabica and C. racemosa. Seeds were first dried to 0.20 g/g (in equilibrium with 78-80% RH). Sufficiently rapid cooling and warming was achieved in hermetically-sealed foil-laminate bags containing 10-11 g (or 50 seeds) by plunging bags directly into liquid nitrogen ? LN containers and placing bags removed from LN directly into a 40 °C bath. Successfully cryopreserved C. arabica and C. racemosa seeds showed minimal viability loss after two-year storage in liquid nitrogen. The same protocol is being adapted to other species of Coffea.
653 $aCoffee germplasm
653 $aGenebanks
700 1 $aRIBEIRO, V. S.
700 1 $aFUZUOLI, L. C.
700 1 $aGUERREIRO FILHO, O.
700 1 $aWALTERS, C.
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