Data/hora: 15/06/2024 07:48:22

Biblioteca(s): | Embrapa Café.
Data corrente: | 04/03/2011
Data da última atualização: | 04/03/2011
Autoria: | SATO, J. H.; TEIXEIRA, J. B.; EIRA, M. T. S. da; FRANCO, O. L.; REIS, A. M. dos.
Afiliação: | UNB; JOAO BATISTA TEIXEIRA, CENARGEN; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, CENARGEN.
Título: | Comparative proteomic analysis of Coffea arabica somatic embryos in the different stages of embryogenesis.
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.
Idioma: | Inglês
Conteúdo: | Coffee is one of the most important cultivated agricultural crops and is of great importance for Brazilian economy. Studies focusing on somatic embryogenesis have been widely performed aiming at the comprehension of the mechanisms involved. Somatic embryogenesis is an important process that helps in vitro micropropagation, which is performed to obtain a high number of plants with high economic value, genetically uniform and free from diseases. The objective of this study was to analyze the protein profile of Coffea arabica cv. Catuaí Vermelho somatic embryos in the stages of torpedo and cotyledonary by two-dimensional electrophoresis (2-DE). Samples were collected and macerated in liquid nitrogen. Protein extraction was performed by using phenol and extraction buffer. The proteins obtained were quantified, analyzed by 2-DE and stained with silver nitrate. The 2D gels revealed proteins varying in size from 10 to 160 kDa and in pI from 3 to 10. The analysis of the protein profiles showed a higher amount of proteins in the cotyledonary phase, especially in the area of pH 3 to 5,5. A total of 45 differentially expressed proteins were observed when comparing both profiles, including 8 proteins observed only in the cotyledonary phase and 4 exclusive to the torpedo phase. Theses proteins will be further identified by mass spectrometry in order to better understand the expression changes during somatic embryogenesis in coffee.
Palavras-Chave: | Coffee; In vitro micropropagation.
Thesaurus Nal: | Somatic embryogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (6-1) - UPC - AA - DD -  -  -  - DIS