Data/hora: 09/06/2024 11:22:26

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	29/02/2008	
Data da última atualização: 	25/09/2019	
Autoria: 	MICLO, L.; GIRARDET, J. -M.; EGITO, A. S. do; MOLLÉ, D.; MARTIN, P.; GAILLARD, J. L.	
Afiliação: 	Miclo L, Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA); Girardet J. M., Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA); ANTONIO SILVIO DO EGITO, CNPC; Mollé D, L'Institut National de la Recherche Agronomique; Martin P., Unité Génomique et Physiologie de la Lactation (GLP) Institut National de la Recherche Agronomique; Gaillard J. L., Unité de Recherche sur l'Animal et les Fonctionnalités des Produits Animaux (UR AFPA L'Institut National de la Recherche Agronomique (INRA).	
Título: 	The primary structure of a low-M(r) multiphosphorylated variant of beta-casein in equine milk.	
Ano de publicação: 	2007	
Fonte/Imprenta: 	Proteomics, v. 7, n. 8, p. 1327-1335, Mar. 2007.	
Idioma: 	Inglês	
Conteúdo: 	Highly phosphorylated casein with a low molecular mass was isolated from Haflinger mare's milk by RP-HPLC. It accounts for 4.0% of the casein content. Its mass was determined by LC-ESI-MS before and after treatment by alkaline phosphatase. The molecular mass found for the apo-form (10 591 ± 2 Da) is in agreement with its primary structure, which was established by ESI-MS/MS from tryptic peptides. It appeared that this short protein (94 amino acid residues) is an internally truncated form of the full-length equine -casein (226 residues). This low-Mr variant of equine -casein displays a large deletion (residues 50-181), due to a cryptic splice site usage occurring within exon 7 during the course of primary transcripts processing. The phosphorylation pattern of this equine -casein variant was investigated by LC-ESI-MS and 2-DE. Seven phosphorylation forms were identified with one to seven phosphate groups with pIs ranging between 4.67 and 4.01. The major isoforms carry five and six phosphate groups.	
Palavras-Chave: 	Equine Milk; Fosforização; High Pressure Liquid; Leite de égua.	
Thesagro: 	Caseína; Enzima.	
Thesaurus Nal: 	Alternative splicing; Amino acid sequences; Beta-casein; Chromatography; Food chemistry; Gel electrophoresis; Horses; Mass spectrometry; Peptides; Phosphorylation; Protein isoforms.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (20714-1) - UPC - AP - DD -  -  -  - DIS