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| Registros recuperados : 74 | |
| 24. |  | OLIVEIRA, J. M. de; NORONHA, A. C. da S.; DUARTE, P. R. M.; CARVALHO, J. E. U. de. Inseto galhador em mudas de bacurizeiro (Platonia insignis). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 25. | | AZEVEDO, A. O.; NORONHA, A. C. S.; FERREIRA, C. T.; OLIVEIRA, F. S.; DUARTE, P. R. M. Ácaros associados a clones de muricizeiro (Byrsonima crassifolia) em Belém e Tomé-Açu, Pará, Brasil. In: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 4., 2013, Bento Gonçalves. Organização, perspectivas e desafios da acarologia brasileira. Bento Gonçalves: [s.n.], 2013.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 26. | | AZEVEDO, A. O.; NORONHA, A. C. S.; FERREIRA, C. T.; OLIVEIRA, F. S.; DUARTE, P. R. M. Ácaros fitoseídeos em muricizeiro (Byrsonima crassifolia) no estado do Pará. In: SIMPÓSIO BRASILEIRO DE ACAROLOGIA, 4., 2013, Bento Gonçalves. Organização, perspectivas e desafios da acarologia brasileira. Bento Gonçalves: [s.n.], 2013.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 28. | | SOUZA, T. M.; MIRANDA, M. Z. de; PRANDO, A. M.; ZUCARELI, C.; HIROOKA, E. Y.; RAYAS-DUARTE, P. Manejo de nitrogênio no teor de amido e propriedades de pasta de trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 12., 2018, Passo Fundo. Ata e Resumos... Passo Fundo: Projeto Passo Fundo, 2019. Melhoramento, Aptidão Industrial e Sementes. p. 421-425.| Biblioteca(s): Embrapa Soja. |
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| 29. | | SOUZA, T. M.; MIRANDA, M. Z. de; PRANDO, A. M.; ZUCARELI, C.; HIROOKA, E. Y.; RAYAS-DUARTE, P. Manejo de nitrogênio no teor de amido e propriedades de pasta de trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 12., 2018, Passo Fundo. Ata e Resumos... Passo Fundo: Projeto Passo Fundo, 2019. Melhoramento, Aptidão Industrial e Sementes, p. 421-425.| Biblioteca(s): Embrapa Trigo. |
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| 30. | | SOUZA, T. M.; MIRANDA, M. Z. de; PRANDO, A. M.; ZUCARELI, C.; HIROOKA, E. Y.; RAYAS-DUARTE, P. Manejo de nitrogênio no teor e propriedades reológicas de proteínas de trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 12., 2018, Passo Fundo. Ata e Resumos... Passo Fundo: Projeto Passo Fundo, 2019. Melhoramento, Aptidão Industrial e Sementes. p. 426-430.| Biblioteca(s): Embrapa Soja. |
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| 31. | | SOUZA, T. M.; MIRANDA, M. Z. de; PRANDO, A. M.; ZUCARELI, C.; HIROOKA, E. Y.; RAYAS-DUARTE, P. Manejo de nitrogênio no teor e propriedades reológicas de proteínas de trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 12., 2018, Passo Fundo. Ata e Resumos... Passo Fundo: Projeto Passo Fundo, 2019. Melhoramento, Aptidão Industrial e Sementes, p. 426-430.| Biblioteca(s): Embrapa Trigo. |
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| 32. | | DUARTE, P. O.; OSHIRO, L. M.; ZIMMERMANN, N. P.; CSORDAS, B. G.; DOURADO, D. M.; BARROS, J. C.; ANDREOTTI, R. Serological and molecular detection of Neospora caninum and Toxoplasma gondii in human umbilical cord blood and placental tissue samples. Scientific Reports, v. 10, n. 9043, 2020.| Biblioteca(s): Embrapa Gado de Corte. |
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| 33. | | SOUZA, T. M.; MIRANDA, M. Z. de; PRANDO, A. M.; TILLEY, M.; PAYTON, M. E.; RAYAS-DUARTE, P. Gluten viscoelasticity: rapid method for classification of soft-like wheat genotypes. Cereal Chemistry, v. 96, n. 2, p. 167-181, Mar./Apr. 2019.| Biblioteca(s): Embrapa Soja; Embrapa Trigo. |
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| Registros recuperados : 74 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARTINS, K. R.; GARCIA, M. V.; BONATTE JUNIOR, P.; DUARTE, P. O.; HIGA, L. O. S. de; CSORDAS, B. G.; BARROS, J. C.; ANDREOTTI, R. |
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Afiliação: |
Kauê Rodriguez Martins; Marcos Valério Garcia; Paulino Bonatte?Junior; Pãmella Oliveira Duarte; Leandro Oliveira Souza de Higa; Bárbara Guimarães Csordas; JACQUELINE CAVALCANTE BARROS, CNPGC; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, jun. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation. MenosThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable ... Mostrar Tudo |
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Palavras-Chave: |
IELISA; QPCR; Tick. |
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Thesagro: |
Cerrado. |
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Thesaurus NAL: |
Anaplasmosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219597/1/16-Correlation-between.pdf
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Marc: |
LEADER 02269naa a2200265 a 4500
001 2128625
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aMARTINS, K. R.
245 $aCorrelation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil.$h[electronic resource]
260 $c2020
520 $aThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation.
650 $aAnaplasmosis
650 $aCerrado
653 $aIELISA
653 $aQPCR
653 $aTick
700 1 $aGARCIA, M. V.
700 1 $aBONATTE JUNIOR, P.
700 1 $aDUARTE, P. O.
700 1 $aHIGA, L. O. S. de
700 1 $aCSORDAS, B. G.
700 1 $aBARROS, J. C.
700 1 $aANDREOTTI, R.
773 $tExperimental and Applied Acarology, jun. 2020.
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