Data/hora: 03/06/2024 06:35:13

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 03/05/2005
Data da última atualização: | 16/08/2018
Autoria: | KÖRBES, A. P.; DROSTE, A.
Afiliação: | Ana Paula Körbes, Universidade do Vale do Rio dos Sinos - Unisinos/Laboratório de Cultura de Tecidos Vegetais; Annette Droste, Universidade do Vale do Rio dos Sinos - Unisinos/Laboratório de Cultura de Tecidos Vegetais.
Título: | Carbon sources and polyethylene glycol on soybean somatic embryo conversion.
Ano de publicação: | 2005
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 3, p. 211-216, mar. 2005
Idioma: | Inglês
Notas: | Título em português: Fontes de carbono e polietilenoglicol na conversão de embriões somáticos de soja.
Conteúdo: | Somatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol (PEG-8000). The histodifferentiated embryos were quantified and classified in morphological types. Percentages of converted embryos were analyzed. Cultivar Bragg resulted in higher matured embryo quantities, but lower percentages were obtained for the conversion in comparison to cultivar IAS5. While the addition of PEG did not affect the number of embryos converted into plants, 6% sucrose enhanced the conversion percent significantly.
Palavras-Chave: | osmotic regulation; PEG-8000; regulação osmótica.
Thesagro: | Glycine Max; Sacarose.
Thesaurus Nal: | sucrose.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/107899/1/Carbon.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (30526-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS