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| Registros recuperados : 25 | |
| 1. |  | SIMÕES FILHO, L. M.; GONZALES, B.; GUIMARÃES, A. S.; DORNELES, E. M. S.; PEREIRA, M. N. Efeito de prebióticos de leveduras sobre o crescimento, ingestão de sucedâneo lácteo, doenças e imunidade de bezerras Holandesas. In: SIMPOSIO ALTA CRIA, 2022, São Carlos. Anais... São Carlos: Alta Genetics, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | BISPO, A. B.; PEREIRA, B. R.; CARNEIRO, G. B.; GONÇALVES, M. S.; DORNELES, E. M. S.; GUIMARÃES, A. S. Susceptibilidade de amostras de Staphylococcus aureus isoladas de mastite bovina a antissépticos utilizados como pré e pós-dipping em fazendas leiteiras. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UFLA, 35., 2022, Lavras. Resumos. Lavras: Universidade Federal de Lavras, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | GONÇALVES, M. S.; DORNELES, E. M. S.; HEINEMANN, M. B.; BRITO, M. A. V. P. e; GUIMARÃES, A. S. Genetic diversity and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis in Minas Gerais, Brazil. Ciência Rural, Santa Maria, v. 53, n. 3, e20210643, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | GONÇALVES, M. S.; OLIVEIRA, M. M. de; ANDRADE, R. S.; OLIVEIRA, L. F. de; GUIMARÃES, A. S.; GODFROID, J.; LAGE, A. P.; DORNELES, E. M. S. Systematic review on the effectiveness of Brucella abortus S19 and RB51 vaccine strains: effectiveness of brucella abortus vaccines. In: ENCONTRO NACIONAL DA REDE EM PESQUISA E INOVAÇÃO EM SANIDADE E PECUÁRIA LEITEIRA, 1., 2023, Lavras. Anais do evento. Lavras: Universidade Federal de Lavras, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | MORAIS-RIOS, P. A. G.; ALVES, T. M.; DORNELES, E. M. S.; STYNEN, A. P. R.; COTTORELLO, A. C. P.; LAURA-FILGUEIRAS, A. L.; LAGE, A. P. Adherence to and invasion of HeLa cells by Campylobacter spp. strains isolated from animals. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1293-1299, julho 2018 Título em português: Adesão e invasão de células HeLa por amostras de Campylobacter spp. isoladas de animais.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | DAMASCENO, M. D.; DORNELES, E. M. S.; GONÇALVES, M. S.; COELHO, M. C.; MORENO, L. Z.; MORENO, A. M.; CARVALHO, B. C. de; GUIMARÃES, A. S. Caracterização microbiológica uterina de vacas leiteiras alojadas em sistemas Compost Barn. In: ENCONTRO NACIONAL DA REDE EM PESQUISA E INOVAÇÃO EM SANIDADE E PECUÁRIA LEITEIRA, 1., 2023, Lavras. Anais do evento. Lavras: Universidade Federal de Lavras, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | GONÇALVES, M. S.; FARIA, J. P. J.; SILVA, J. R.; CUSTÓDIO, D. A. C.; RIBEIRO, J. B.; GUIMARÃES, A. S.; DORNELES, E. M. S.; COSTA, G. M. Flagella are an important virulence factor in the subclinical persistence of Escherichia coli in bovine mammary gland. Journal of Dairy Research, v. 90, n. 2, p. 146-151, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | SANTOS, V. E. C.; BISPO, A. B.; GONÇALVES, M. S.; GUIMARÃES, A. S.; COSTA, G. M. da; DORNELES, E. M. S. Frequência de patógenos da mastite bovina em rebanhos de Minas Gerais. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UFLA, 35., 2022, Lavras. Anais... Lavras: Universidade Federal de Lavras, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | RAMOS, C. P.; DORNELES, E. M. S.; HAAS, D. J.; VESCHI, J. L. A.; LOUREIRO, D.; PORTELA, R. D.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. Ciência Rural, v. 52, n. 11, e20210328, 2022.| Biblioteca(s): Embrapa Semiárido. |
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| 10. | | GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | DORNELES, E. M. S.; MORCATTI, F. C.; GUIMARAES, A. S.; LOBATO, Z. I. P.; LAGE, A. P.; GONÇALVES, V. S. P.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Prevalência de anticorpos contra o virus da lingua azul em ovinos do Distrito Federal. Semina, v. 33, n. 4, p. 1521-1524, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | CARNEIRO, G. B.; GONÇALVES, M. S.; CUSTÓDIO, D. A. da C.; GUIMARÃES, A. S.; COSTA, G. M. da; DORNELES, E. M. S. Resistência à colistina em amostras de Escherichia coli isolada de leite e fezes bovinas. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UFLA, 35., 2022, Lavras. Resumos. Lavras: Universidade Federal de Lavras, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | GONÇALVES, M. S.; COSTA, D. A. C. da; PEREIRA, C. R.; ANDRADE, R. da S.; COSTA, A. C. T. R. B.; COASTA, G. M. da; DORNELES, E. M. S.; GUIMARÃES, A. S. Colistin resistance in Escherichia coli isolated from bovine milk and feces. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE, 9., 2022, Goiânia. Inovação, sustentabilidade e oportunidades: anais. Curitiba: Conselho Brasileiro de Qualidade do Leite, 2022. p. 30-32.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | DORNELES, E. M. S.; PELLEGRIN, A. O.; PERES, I. A. H. F. S.; MATHIAS, L. A.; MOURAO, G. de M.; BIANCHI, R. de C.; OLIFIERS, N.; ROCHA, F. L.; LAGE, A, P. Serology for brucellosis in free-ranging crab-eating foxes (Cerdocyon thous) and brown-nosed coatis (Nasua nasua) from Brazilian Pantanal. Ciência Rural, Santa Maria, v. 44, n. 12, p. 2193-2196, dez. 2014.| Biblioteca(s): Embrapa Pantanal. |
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| 16. | | DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B. Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil. Microbiology Open, v. 8, n. 5, article e736, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | PÉREZ, V. K. C.; MAGOSSI, E. M.; OLIVEIRA, P. F. R. e; CUSTODIO, D. A. C.; CRUZ, H. F. DA; ABREU, J. P. DE; HEINEMANN, M. B.; GUIMARÃES, A. S.; DORNELES, E. M. Detection of biofilm-forming Staphylococcus aureus isolated from bovine mastitis and association with antimicrobial resistance in Brazil. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGIA, 24., 2018, Santiago, Chile. Resúmenes... Santiago: ALAM, 2018. p. 201.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | DORNELES, E. M. S.; ANDRADE, G. I.; SANTOS, E. L. DE S.; SANTANA, J. A.; LAGE, A. P.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. DE S.; MIYOSHI, A.; AZEVEDO, V. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. In: Encontro Nacional de Patologia Veterinária, 15., 2011, Goiânia. Resumos...| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | DORNELES, E. M. S.; ANDRADE, G. I.; SANTOS, E. L. DE S.; SANTANA, J. A.; LAGE, A. P.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. DE S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using eric-PCR. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | DORNELES, E. M. S.; SANTANA, J. A.; ANDRADE, G. I.; SANTOS, E. L. S.; GUIMARAES, A. S.; MOTA, R. A.; SANTOS, A. S.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; LAGE, A. P.; HEINEMANN, M. B. Molecular characterization of Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR. Genetics and Molecular Research, v. 153, n. 3/4, p. 299-306, 2011| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 25 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
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Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
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Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
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Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
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Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
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Thesaurus NAL: |
Biofilm; Hemolysins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03482naa a2200373 a 4500
001 2133706
005 2021-08-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI
100 1 $aPEREZ, V. K. C.
245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource]
260 $c2020
520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin.
650 $aBiofilm
650 $aHemolysins
650 $aBiofilme
650 $aBovino
650 $aDoença Animal
650 $aEstafilococo
653 $aHemolisina
653 $aInfecção intramamária
653 $aIntramammary infection
653 $aMastite
653 $aStaphylococci
700 1 $aCUSTODIO, D. A. C.
700 1 $aSILVA, E. M. M.
700 1 $aOLIVEIRA, J. de
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aSOUZA-FILHO, A. F.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
700 1 $aDORNELES, E. M. S.
773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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