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| Registros recuperados : 46 | |
| 2. |  | IVAMOTO, S. T.; REIS JUNIOR, O.; CARAZZOLLE, M. F.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptoma de frutos de Coffea arabica L. ao longo do seu desenvolvimento inicial. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Biblioteca(s): Embrapa Café. |
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| 4. | | PEREIRA, L. F. P.; YANAGUI, K.; FERREIRA, L. P.; DOMINGUES, D. S.; VIEIRA, L. G. E.; POT, D. Analysis Of Nucleotide Diversity In Coffea spp. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 19, 2011, San Diego, CA.| Biblioteca(s): Embrapa Café. |
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| 5. | | DOMINGUES, D. S.; FARRO, A. P. C.; COSCRATO, V. E.; MELLO, E. J. de; ODA, S.; MARINO, C. L. Identificação de marcadores RAPD e SCAR relacionados ao caractere florescimento precoce em Eucalyptus grandis. Ciência Florestal, Santa Maria, v. 16, n. 3/4, p. 251-260, set. 2006.| Biblioteca(s): Embrapa Florestas. |
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| 6. | | FERREIRA, R. V.; ANDRADE, G. A.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D. S.; PEREIRA, L. F. P. Identificação de novos acessos de Coffea Arábica introduzidos na coleção da Etiópia do IAPAR. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 9., 2015, Curitiba. Anais... Brasília, DF: Embrapa Café, 2015.| Biblioteca(s): Embrapa Café. |
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| 8. | | IVAMOTO, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; DOMINGUES, D. S.; PEREIRA, L. F. P.; BOHLMANN, J. Biochemical and molecular analysis of Coffea arabica to identify candidate genes related to diterpenes biosynthesis. In: INTERNATIONAL MEETING ON BIOSYNTHESIS, FUNCTIONS AND SYNTHETIC BIOLOGY OF ISOPRENOIDS, 12., 2015, Vancouver, Canada. Porter... Vancouver, Canada: University of British Columbia, 2015.| Biblioteca(s): Embrapa Café. |
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| 9. | | PASCHOAL, A. R.; FERNANDES, E. D. M.; SILVA, J. C.; LOPES, F. M.; PEREIRA, L. F. P.; DOMINGUES, D. S. CoffeebEST: an integrated resource for Coffea spp expressed sequence tags. Genetics and Molecular Research, v. 13, n. 4, p. 10913-10920, 2014.| Biblioteca(s): Embrapa Café. |
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| 10. | | IVAMOTO, S. T.; POT, D.; LANNES, S. D.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Diversidade nucleotídica de genes envolvidos na biossíntese de ácidos clorogênicos de cafeeiros. Coffee Science, Lavras, v. 8, n. 2, p. 148-156, abr./jun. 2013.| Biblioteca(s): Embrapa Café. |
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| 11. | | IVAMOTO, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; DOMINGUES, D. S.; PEREIRA, L. F. P.; BOHLMANN, J. Molecular and metabolite analyses of Coffea arabica L. fruits to identify candidate genes for diterpene biosynthesis. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls, Brazil. Summaries... Rockville, USA: American Society of Plant Biologists, 2015.| Biblioteca(s): Embrapa Café. |
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| 13. | | IVAMOTO, S. T.; POT, D.; FERREIRA, L. P.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Análise in silico da expressão gênica de citocromo p450 relacionada ao metabolismo de diterpenos em Coffea. In.: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá.| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 14. | | SANT'ANA, G. C.; POT, D.; IVAMOTO, S. T.; DOMINGUES, D. S.; SCHOLZ, M. B. B.; LEROY, T.; PEREIRA, L. F. P. Genomic regions associated to lipids and diterpenes contents slected during domestication and breeding of Coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 72| Biblioteca(s): Embrapa Café. |
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| 15. | | SOUZA, S. G. H. de; SANTOS, T. B. dos; DOMINGUES, D. S.; BERNADAC, A.; BOUZAYEN, M.; PEREIRA, L. F. P.; DEGRASSI, G.; CARPENTIERI-PIPOLO, V. Identification, structure analyses and expression pattern of the ERF transcription factor family in coffea arabica. Journal of Botanical Research, v. 3, n.1, Jan. 2021.| Biblioteca(s): Embrapa Café; Embrapa Trigo. |
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| 16. | | SILVA, B. S. R. da; CAÇÃO, S. B.; IVAMOTO, S. T.; SILVA, J. C.; DOMINGUES, D. S.; PEREIRA, L. F. P. Identificação e caracterização de microssatélites de Coffea arabica a partir de dados de sequenciamento de RNA e de BACS. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Biblioteca(s): Embrapa Café. |
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| 17. | | CARVALHO, K. de; PETKOWICZ, C. L. O.; NAGASHIMA, G. T.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. Homeologous genes involved in mannitol synthesis reveal unequal contributions in response to abiotic stress in Coffea arábica. Molecular Genetics and Genomics, v. 289, n. 5, p. 951-963, 2014.| Biblioteca(s): Embrapa Café. |
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| 18. | | IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P. Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L. Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023.| Biblioteca(s): Embrapa Café. |
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| 19. | | DOMINGUES, D. S.; REIS JUNIOR, O.; SANTOS, T. B. dos; SILVA, J. C.; LORENZETTI, A. P.; SOARES, J. D. M.; PEREIRA, L. F. P. Nitrogen-starvation responsive microRNAs in Coffea arabica roots: its this a 3-player game? In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls, Brazil. Summaries... Rockville, USA: American Society of Plant Biologists, 2015.| Biblioteca(s): Embrapa Café. |
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| 20. | | CARVALHO, K. de; BESPALHOK FILHO, J. C.; SANTOS, T. B. dos; SOUZA, S. G. H. de; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. Nitrogen starvation, salt and heat stress in coffee (coffea arabica L.): identification and validation of new genes for qPCR normalization. Molecular Biotechnology, v. 53, n. 3, p. 315-325, 2013.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 46 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/01/2024 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAÇÃO, S. M. B.; LEITE, T. F.; BUDZINSKI, I. G. F.; SANTOS, T. B.; SCHOLZ, M. B. S.; CARPENTIERI-PIPOLO, V.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. |
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Afiliação: |
S. M. B. CAÇÃO, INSTITUTO AGRONÔMICO DO PARANÁ; T. F. LEITE, INSTITUTO AGRONÔMICO DO PARANÁ; I. G. F. BUDZINSKI, INSTITUTO AGRONÔMICO DO PARANÁ; T. B. DOS SANTOS, INSTITUTO AGRONÔMICO DO PARANÁ; M. B. S. SCHOLZ, INSTITUTO AGRONÔMICO DO PARANÁ; V. CARPENTIERI-PIPOLO, UNIVERSIDADE ESTADUAL DE LONDRINA; D. S. DOMINGUES, INSTITUTO AGRONÔMICO DO PARANÁ; L. G. E. VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa. |
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Título: |
Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3186-3197, 2012. |
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DOI: |
http://dx.doi.org/10.4238/2012.September.3.7 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring during coffee fruit maturation, the enzymatic activity of PME was measured during different stages of fruit ripening. PME activity progressively increased from the beginning of the ripening process to the cherry fruit stage. In silico analysis of expressed sequence tags of the Brazilian Coffee Genome Project database identified 5 isoforms of PME. We isolated and cloned a cDNA homolog of PME for further characterization. CaPME4 transcription was analyzed in pericarp, perisperm, and endosperm tissues during fruit development and ripening as well as in other plant tissues. Northern blot analysis revealed increased transcription of CaPME4 in the pericarp 300 days after flowering. Low levels of CaPME4 mRNAs were observed in the endosperm 270 days after flowering. Expression of CaPME4 transcripts was strong in the branches and lower in root and flower tissues. We showed that CaPME4 acts specifically during the later stages of fruit ripening and possibly contributes to the softening of coffee fruit, thus playing a significant role in pectin degradation in the fruit pericarp. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus NAL: |
Gene expression; Plant development; Ripening; Seed maturation; Seed quality. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160536/1/Gene-expression.pdf
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Marc: |
LEADER 02430naa a2200301 a 4500
001 2160536
005 2024-01-08
008 2012 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2012.September.3.7$2DOI
100 1 $aCAÇÃO, S. M. B.
245 $aGene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.$h[electronic resource]
260 $c2012
520 $aCoffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to characterize the changes occurring during coffee fruit maturation, the enzymatic activity of PME was measured during different stages of fruit ripening. PME activity progressively increased from the beginning of the ripening process to the cherry fruit stage. In silico analysis of expressed sequence tags of the Brazilian Coffee Genome Project database identified 5 isoforms of PME. We isolated and cloned a cDNA homolog of PME for further characterization. CaPME4 transcription was analyzed in pericarp, perisperm, and endosperm tissues during fruit development and ripening as well as in other plant tissues. Northern blot analysis revealed increased transcription of CaPME4 in the pericarp 300 days after flowering. Low levels of CaPME4 mRNAs were observed in the endosperm 270 days after flowering. Expression of CaPME4 transcripts was strong in the branches and lower in root and flower tissues. We showed that CaPME4 acts specifically during the later stages of fruit ripening and possibly contributes to the softening of coffee fruit, thus playing a significant role in pectin degradation in the fruit pericarp.
650 $aGene expression
650 $aPlant development
650 $aRipening
650 $aSeed maturation
650 $aSeed quality
650 $aCoffea Arábica
700 1 $aLEITE, T. F.
700 1 $aBUDZINSKI, I. G. F.
700 1 $aSANTOS, T. B.
700 1 $aSCHOLZ, M. B. S.
700 1 $aCARPENTIERI-PIPOLO, V.
700 1 $aDOMINGUES, D. S.
700 1 $aVIEIRA, L. G. E.
700 1 $aPEREIRA, L. F. P.
773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3186-3197, 2012.
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