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 | Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
07/10/2021 |
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Data da última atualização: |
08/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VOLPON, A. G. T.; DE POLLI, H.; DOBEREINER, J. |
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Afiliação: |
ANTONIA G. T. VOLPON, UFRRJ; HELVECIO DE POLLI, SNLCS; JOHANNA DOBEREINER, SNLCS. |
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Título: |
Physiology of nitrogen fixation in Azospirillum lipoferum Br 17 (ATCC 29709). |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 128, n. 4, p. 371-375, 1981. |
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DOI: |
https://doi.org/10.1007/BF00405915 |
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Idioma: |
Inglês |
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Conteúdo: |
Changes of cellular activities during batch cultures with Azospirillum lipoferum strain Br 17 (ATCC 29 709) were observed within the growth cycle, at optimal pO2 (0.002-0.003 atm). The relative growth rate for cells growing with N2 as sole nitrogen source during log phase was u=0.13 h-1 and the doubling time was 5.3 h. Nitrogenase activity was not accompanied by hydrogen evolution at any growth stage, and a very active uptake hydrogenase was demonstrated. The hydrogenase activity increased towards the end of the growth period when glucose became limiting and N2 fixation reached its maximal specific activity. Oxygen consumption and oxygen tolerance at the various growth stages, increased simultaneously with the uptake hydrogenase activity indicating a possible role of this enzyme in an oxygen protection mechanism of A. lipoferum nitrogenase. The efficiency of nitrogen fixation expressed as mg total nitrogen fixed in cells and supernatant per g glucose consumed, was 20 at the early log phase and increased to 48 at the late log phase. About 25% of the total fixed nitrogen was recovered in the culture supernatant. |
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Palavras-Chave: |
Efficiency; Hydrogenase; Oxygen protection. |
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Thesaurus NAL: |
Azospirillum. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01745naa a2200205 a 4500
001 2135172
005 2021-10-08
008 1981 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF00405915$2DOI
100 1 $aVOLPON, A. G. T.
245 $aPhysiology of nitrogen fixation in Azospirillum lipoferum Br 17 (ATCC 29709).$h[electronic resource]
260 $c1981
520 $aChanges of cellular activities during batch cultures with Azospirillum lipoferum strain Br 17 (ATCC 29 709) were observed within the growth cycle, at optimal pO2 (0.002-0.003 atm). The relative growth rate for cells growing with N2 as sole nitrogen source during log phase was u=0.13 h-1 and the doubling time was 5.3 h. Nitrogenase activity was not accompanied by hydrogen evolution at any growth stage, and a very active uptake hydrogenase was demonstrated. The hydrogenase activity increased towards the end of the growth period when glucose became limiting and N2 fixation reached its maximal specific activity. Oxygen consumption and oxygen tolerance at the various growth stages, increased simultaneously with the uptake hydrogenase activity indicating a possible role of this enzyme in an oxygen protection mechanism of A. lipoferum nitrogenase. The efficiency of nitrogen fixation expressed as mg total nitrogen fixed in cells and supernatant per g glucose consumed, was 20 at the early log phase and increased to 48 at the late log phase. About 25% of the total fixed nitrogen was recovered in the culture supernatant.
650 $aAzospirillum
653 $aEfficiency
653 $aHydrogenase
653 $aOxygen protection
700 1 $aDE POLLI, H.
700 1 $aDOBEREINER, J.
773 $tArchives of Microbiology$gv. 128, n. 4, p. 371-375, 1981.
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