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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
20/06/2011 |
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Data da última atualização: |
12/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FILIPPI, M. C. C.; SILVA, G. B. da; SILVA-LOBO, V. L.; CORTES, M. V. C. B.; MORAES, A. J. G.; PRABHU, A. S. |
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Afiliação: |
MARTA CRISTINA CORSI DE FILIPPI, CNPAF; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; VALACIA LEMES DA SILVA LOBO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ALESSANDRA JACKELINE G. MORAES, UNIVERSIDADE FEDERAL RURAL DA AMAZÔNIA; ANNE SITARAMA PRABHU. |
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Título: |
Leaf blast (Magnaporthe oryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Biological Control, San Diego, v. 58, n. 2, p. 160-166, Aug. 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Rice blast caused by Magnaporthe oryzae has the potential to cause 100% grain yield loss. The objective of this investigation was to identify rhizobacteria showing potential for plant growth stimulation and resistance induction under greenhouse conditions. Bacterial isolates were collected from the rhizosphere of rice plants from soils of Amazon, PA. Soil application with rhizobacteria was done by drenching with bacterial cell suspension before inoculating with virulent isolate of M. oryzae. Mass screening of 148 isolates for growth promotion showed that 12.7% stimulated plant height, whereas 52.0% increased root length, total biomass and root biomass. Based on these growth promotion results, 18 isolates were further tested for in vitro inhibition of the pathogen and reduction of leaf blast severity (LBS) in greenhouse test. All isolates inhibited pathogen growth and reduced disease severity from 16% to 95%. The two isolates showing the greatest suppression of leaf blast (Rizo-46 and Rizo-55) were further tested in a subsequent greenhouse trial using three replications and three application methods (drenching the soil, 15 and 2 days before inoculation with rice pathogen, and spraying 2 days before inoculating with virulent isolate). The soil drenching with isolate Rizo-55, 15 days prior to challenging with virulent isolate of M. oryzae reduced LBS by 90%, whereas Rizo-46 applied 2 days before reduced LBS by 95%. The capacity to suppress leaf blast by isolates Rizo-46 and Rizo-55 varied according to the mode of application. Also, the enzymatic tests were conducted to quantitate the presence of proteins related to pathogenesis (PRPs) during induction process of resistance by rhizobacteria. The enzyme activity of peroxidase, b-1,3-glucanase and chitinase greatly increased, and the results are in accord with greenhouse tests in relation to leaf blast disease suppression. MenosRice blast caused by Magnaporthe oryzae has the potential to cause 100% grain yield loss. The objective of this investigation was to identify rhizobacteria showing potential for plant growth stimulation and resistance induction under greenhouse conditions. Bacterial isolates were collected from the rhizosphere of rice plants from soils of Amazon, PA. Soil application with rhizobacteria was done by drenching with bacterial cell suspension before inoculating with virulent isolate of M. oryzae. Mass screening of 148 isolates for growth promotion showed that 12.7% stimulated plant height, whereas 52.0% increased root length, total biomass and root biomass. Based on these growth promotion results, 18 isolates were further tested for in vitro inhibition of the pathogen and reduction of leaf blast severity (LBS) in greenhouse test. All isolates inhibited pathogen growth and reduced disease severity from 16% to 95%. The two isolates showing the greatest suppression of leaf blast (Rizo-46 and Rizo-55) were further tested in a subsequent greenhouse trial using three replications and three application methods (drenching the soil, 15 and 2 days before inoculation with rice pathogen, and spraying 2 days before inoculating with virulent isolate). The soil drenching with isolate Rizo-55, 15 days prior to challenging with virulent isolate of M. oryzae reduced LBS by 90%, whereas Rizo-46 applied 2 days before reduced LBS by 95%. The capacity to suppress leaf blast by isolates Rizo-46 and Riz... Mostrar Tudo |
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Thesagro: |
Arroz; Brusone; Doença de planta; Oryza sativa. |
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Thesaurus Nal: |
Magnaporthe oryzae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02610naa a2200241 a 4500
001 1894505
005 2014-12-12
008 2011 bl uuuu u00u1 u #d
100 1 $aFILIPPI, M. C. C.
245 $aLeaf blast (Magnaporthe oryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil.
260 $c2011
520 $aRice blast caused by Magnaporthe oryzae has the potential to cause 100% grain yield loss. The objective of this investigation was to identify rhizobacteria showing potential for plant growth stimulation and resistance induction under greenhouse conditions. Bacterial isolates were collected from the rhizosphere of rice plants from soils of Amazon, PA. Soil application with rhizobacteria was done by drenching with bacterial cell suspension before inoculating with virulent isolate of M. oryzae. Mass screening of 148 isolates for growth promotion showed that 12.7% stimulated plant height, whereas 52.0% increased root length, total biomass and root biomass. Based on these growth promotion results, 18 isolates were further tested for in vitro inhibition of the pathogen and reduction of leaf blast severity (LBS) in greenhouse test. All isolates inhibited pathogen growth and reduced disease severity from 16% to 95%. The two isolates showing the greatest suppression of leaf blast (Rizo-46 and Rizo-55) were further tested in a subsequent greenhouse trial using three replications and three application methods (drenching the soil, 15 and 2 days before inoculation with rice pathogen, and spraying 2 days before inoculating with virulent isolate). The soil drenching with isolate Rizo-55, 15 days prior to challenging with virulent isolate of M. oryzae reduced LBS by 90%, whereas Rizo-46 applied 2 days before reduced LBS by 95%. The capacity to suppress leaf blast by isolates Rizo-46 and Rizo-55 varied according to the mode of application. Also, the enzymatic tests were conducted to quantitate the presence of proteins related to pathogenesis (PRPs) during induction process of resistance by rhizobacteria. The enzyme activity of peroxidase, b-1,3-glucanase and chitinase greatly increased, and the results are in accord with greenhouse tests in relation to leaf blast disease suppression.
650 $aMagnaporthe oryzae
650 $aArroz
650 $aBrusone
650 $aDoença de planta
650 $aOryza sativa
700 1 $aSILVA, G. B. da
700 1 $aSILVA-LOBO, V. L.
700 1 $aCORTES, M. V. C. B.
700 1 $aMORAES, A. J. G.
700 1 $aPRABHU, A. S.
773 $tBiological Control, San Diego$gv. 58, n. 2, p. 160-166, Aug. 2011.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
08/11/2012 |
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Data da última atualização: |
24/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PIMENTA-MARTINS, M. G. R.; FURTADO, R. F.; HENEINE, L. G. D.; DIAS, R. S.; BORGES, M. de F.; ALVES, C. A. |
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Afiliação: |
Maria Gardenny Ribeiro Pimenta-Martins, UECE; ROSELAYNE FERRO FURTADO, CNPAT; Luiz Guilherme Dias Heneine, FUNED; Ricardo Souza Dias, FUNED; MARIA DE FATIMA BORGES, CNPAT; Carlucio Roberto Alves, UECE. |
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Título: |
Development of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Microbiological Methods, v.91, p.138-143, 2012. |
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Idioma: |
Português |
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Conteúdo: |
This paper reports a novel electrochemical immunosensorforthe sensitive detection of staphylococcal enterotox in A (S EA ) based on self-assembly monolayer (SA M) and p rotein A i mm obilizat io n on gold electro de. Three diffe rent methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modi fied gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and speci ficity. The proposed amperometric immuno- sensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses. |
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Palavras-Chave: |
Biosensor; Staphylococcal enterotoxin A. |
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Thesaurus NAL: |
food safety. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01660naa a2200217 a 4500
001 1939204
005 2013-04-24
008 2012 bl uuuu u00u1 u #d
100 1 $aPIMENTA-MARTINS, M. G. R.
245 $aDevelopment of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese.
260 $c2012
520 $aThis paper reports a novel electrochemical immunosensorforthe sensitive detection of staphylococcal enterotox in A (S EA ) based on self-assembly monolayer (SA M) and p rotein A i mm obilizat io n on gold electro de. Three diffe rent methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modi fied gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetry and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and speci ficity. The proposed amperometric immuno- sensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses.
650 $afood safety
653 $aBiosensor
653 $aStaphylococcal enterotoxin A
700 1 $aFURTADO, R. F.
700 1 $aHENEINE, L. G. D.
700 1 $aDIAS, R. S.
700 1 $aBORGES, M. de F.
700 1 $aALVES, C. A.
773 $tJournal of Microbiological Methods$gv.91, p.138-143, 2012.
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Embrapa Agroindústria Tropical (CNPAT) |
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