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| Registros recuperados : 178 | |
| Registros recuperados : 178 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
20/12/2018 |
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Data da última atualização: |
20/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
GRUNENNVALDT, R. L.; DEGENHARDT-GOLDBACH, J.; TOMASI, J. de C.; SANTOS, G. D. dos; VICENTE, V. A.; DESCHAMPS, C. |
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Afiliação: |
Renata Lúcia Grunennvaldt, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; Jéssica de Cássia Tomasi, UFPR; Germana Davila dos Santos, UFPR; Vânia Aparecida Vicente, UFPR; Cícero Deschamps, UFPR. |
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Título: |
Bacillus megaterium: an endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biotecnología Vegetal, v. 18, n. 1, p. 3-13, ene./mar. 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Endophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests. |
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Palavras-Chave: |
16S rRNA; Erva mate; Sequencing. |
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Thesagro: |
Biotecnologia; Cultura de Tecido; Espécie Nativa; Ilex Paraguariensis. |
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Thesaurus NAL: |
Tissue culture. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189023/1/2018-Juliana-BV-Bacillus.pdf
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Marc: |
LEADER 01716naa a2200277 a 4500
001 2102141
005 2018-12-20
008 2018 bl uuuu u00u1 u #d
100 1 $aGRUNENNVALDT, R. L.
245 $aBacillus megaterium$ban endophytic bacteria from callus of Ilex paraguariensis with growth promotion activities.$h[electronic resource]
260 $c2018
520 $aEndophytic microorganisms live inside healthy plants and their isolation and identification may favor biotechnological applications. We isolated endophytic bacteria from Ilex paraguariensis callus cultivated in vitro for five months. Sequencing of 16S rRNA region to identify two isolates as Bacillus megaterium was used. The presence of bacterial cells in the intercellular spaces of callus cultures was detected by ultra structural analyses. The isolates were also evaluated for indole acetic acid (IAA) production and their potential in plant growth promotion of Phaseolus vulgaris L. The synthesis of IAA in the presence and absence of L-tryptophan was detected by colorimetric analyze for both isolates. In the presence of extracts from strains IPC06 and IPC07 the seedlings of P. vulgaris grew faster when compared to the control plants without the extract on in vitro tests.
650 $aTissue culture
650 $aBiotecnologia
650 $aCultura de Tecido
650 $aEspécie Nativa
650 $aIlex Paraguariensis
653 $a16S rRNA
653 $aErva mate
653 $aSequencing
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aTOMASI, J. de C.
700 1 $aSANTOS, G. D. dos
700 1 $aVICENTE, V. A.
700 1 $aDESCHAMPS, C.
773 $tBiotecnología Vegetal$gv. 18, n. 1, p. 3-13, ene./mar. 2018.
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Registro original: |
Embrapa Florestas (CNPF) |
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