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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
05/03/2024 |
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Data da última atualização: |
05/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, H. A. P. da; CAETANO, V. S.; PESSÔA, D. D. V.; PACHECO, R. S.; MENESES, C. H. S. G.; ARAUJO, J. L. S. de. |
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Afiliação: |
HELDER ANDERSON PINTO DA SILVA, UFRJ; VANESSA SANTANA CAETANO, UFRJ; DANIELLA DUARTE VILLARINHO PESSÔA, UFRJ; RAFAEL SANCHES PACHECO, UFRRJ; CARLOS HENRIQUE S. G. MENESES, UEPB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Unraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 152024, 1345379, 2024 |
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ISSN: |
1664462X |
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DOI: |
10.3389/fpls.2024.1345379 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions MenosCommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to ... Mostrar Tudo |
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Palavras-Chave: |
Candidate genes; Genetic transcript profile; RT-qPCR; Symbiotic nitrogen assimilation; Water deficit. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03111naa a2200265 a 4500
001 2162595
005 2024-03-05
008 2024 bl uuuu u00u1 u #d
022 $a1664462X
024 7 $a10.3389/fpls.2024.1345379$2DOI
100 1 $aSILVA, H. A. P. da
245 $aUnraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation.$h[electronic resource]
260 $c2024
520 $aCommon bean (Phaseolus vulgaris) can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance. The RNA-Seq analysis yielded a total of 81,489,262 and 72,497,478 high quality reads for Negro Argel and BAT 477 genotypes, respectively. The reads were mapped to the Phaseolus vulgaris reference genome and expression analysis identified 145 and 1451 differentially expressed genes (DEGs) for Negro Argel and BAT 477 genotypes, respectively. Although BAT 477 had more DEGs, both genotypes shared certain drought-responsive genes, including an up-regulated heat shock protein (HSP) and a down-regulated peroxidase, indicating shared pathways activated during drought in nodule tissue. Functional analysis using MapMan software highlighted the up-regulation of genes involved in abiotic stress responses, such as HSPs and specific transcription factors (TFs), in both genotypes. There was a significant down-regulation in metabolic pathways related to antioxidant protection, hormone signaling, metabolism, and transcriptional regulation. To validate these findings, we conducted RT-qPCR experiments for ten DEGs in nodules from both genotypes, for which the expression profile was confirmed, thus reinforcing their functional relevance in the nodule responses to drought stress during BNF. BAT 477 genotype exhibited more pronounced response to drought, characterized by a high number of DEGs. The strong down-regulation of DEGs leads to transcriptional disturbances in several pathways related to stress acclimation such as hormone and antioxidant metabolism. Additionally, we identified several genes that are known to play key roles in enhancing drought tolerance, such as HSPs and crucial TFs. Our results provide new insights into the transcriptional responses in root-nodules, an underexplored tissue of plants mainly under drought conditions. This research paves the way for potential improvements in plant-bacteria interactions, contributing to common bean adaptations in the face of challenging environmental conditions
653 $aCandidate genes
653 $aGenetic transcript profile
653 $aRT-qPCR
653 $aSymbiotic nitrogen assimilation
653 $aWater deficit
700 1 $aCAETANO, V. S.
700 1 $aPESSÔA, D. D. V.
700 1 $aPACHECO, R. S.
700 1 $aMENESES, C. H. S. G.
700 1 $aARAUJO, J. L. S. de
773 $tFrontiers in Plant Science$gv. 152024, 1345379, 2024
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Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
19/02/2015 |
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Data da última atualização: |
20/02/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SUGAI, L. S. M.; COSTA-PEREIRA, R.; QUINTEIRO, J. M. O.; TORRECILHA, S.; ERIKSSON, A.; NUNES, A. P.; KEUROGHLIAN, A.; ARAUJO, A. C.; PARANHOS FILHO, A. C.; DESBIEZ, A. L. J.; POTT, A.; SANTOS, C. F.; FERREIRA, C. M. M.; RIBEIRO, D. B.; EATON, D. P.; SCREMIN-DIAS, E.; CORRÊA, EL. C.; BURKHARDT, E.; FISCHER, E.; AYRES, F. M.; MARTINS, F. I.; ALVES, F. M.; SEVERO-NETO, F.; SOUZA, F. L.; PAULA, G. A. DE.; SOUZA, G. N. de; SEIXAS, G. H. F.; DALPONTI, G.; BARROS, G. M. de; GRACIOLLI, G.; GIMÊNES JUNIOR, H.; ILHA, I. M. N.; SUGAI, J. L. M. M.; LONGO, J. M.; SABINO, J.; CORDEIRO, L. M.; NASCIMENTO, L. N.; TENCATT, L. F. C.; BUENO, M. L.; RODRIGUES, M. E.; BENITES, M.; SILVA, M.; GODOI, M. N.; SILVEIRA, M.; DELATORRE, M.; RIBEIRO, M. C.; GUEDES, N. M. R.; CUNHA, N. L. da; FROEHLICH, O.; MEDICI, P.; MEDINA JUNIOR, P. B.; SOUZA, P. R. de; FERREIRA, R. P.; LOURIVAL, R. F. F.; GAMARRA, R. M.; BORGHEZAN, R.; LAPS, R. R.; ROCHA, S. B. da; DULEBA, S.; MAMEDE, S.; ESCAPINATI, S. C.; SINANI, T. R. F.; FERREIRA, V. L.; TOMAS, W. M.; SÚAREZ, Y. R.; ROQUE, F. de O. |
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Afiliação: |
LARISSA SAYURI MOREIRA SUGAI, UFMS; RAUL COSTA-PEREIRA, UNESP; JOSE MANUEL OCHOA QUINTERO, UFMS; SYLVIA TORRECILHA, UFMS; ALAN ERIKSSON, UFMS; ALESSANDRO PACHECO NUNES, UFMS; ALEXINE KEUROGHLIAN, WILDLIFE CONSERVATION SOCIETY; ANDRÉA CARDOSO ARAUJO, UFMS; ANTONIO CONCEIÇÃO PARANHOS FILHO, UFMS; ARNAUD LÉONARD JEAN DESBIEZ, INSTITUTO DE PESQUISAS ECOLÓGICAS - IPÊ; ARNILDO POTT, UFMS; CAROLINA FERREIRA SANTOS, UFMS; CLÁUDIA MÁRCIA MARILY FERREIRA, UFMS; DANILO BANDINI RIBEIRO, UFMS; DONALD PARSONS EATON, WILDLIFE CONSERVATION SOCIETY; EDNA SCREMIN-DIAS, UFMS; ELAINE CRISTINA CORRÊA, UFMS; ELIZABETE BURKHARDT, IMASUL; ERICH FISCHER, UFMS; FABIO MARTINS AYRES, IMASUL; FERNANDO IBANEZ MARTINS, UFMS; FLÁVIO MACEDO ALVES, UFMS; FRANCISCO SEVERO-NETO, UFMS; FRANCO LEANDRO SOUZA, UFMS; GABRIEL ARVELINO DE PAULA, UFMS; GABRIEL NAKAMURA DE SOUZA, UFMS; GLÁUCIA HELENA FERNANDES SEIXAS, FUNDAÇÃO NEOTRÓPICA DO BRASIL; GUILHERME DALPONTI, UFMS; GUILHERME MAIER DE BARROS, UFMS; GUSTAVO GRACIOLLI, UFMS; HERIBERTO GIMÊNES JUNIOR, UFMS; IEDA MARIA NOVAES ILHA, UFMS; JOSE LUIZ MASSAO MOREIRA SUGAI, UFMS; JOSÉ MILTON LONGO, FIBRACON CONSULTORIA, PERÍCIAS E PROJETOS AMBIENTAIS; JOSÉ SABINO, UNIDERP; LÍVIA MEDEIROS CORDEIRO, UFMS; LUANA NAYARA NASCIMENTO, UFMS; LUIZ FERNANDO CASERTA TENCATT, NUPÉLIA; MARCELO LEANDRO BUENO, UFMG; MARCIEL ELIO RODRIGUES, UFMS; MARISTELA BENITES; MARIZA SILVA, FUNDAÇÃO NEOTRÓPICA DO BRASIL; MAURICIO NEVES GODOI, UFMS; MAURICIO SILVEIRA, UFMS; MILENA DELATORRE, UFMS; MILTON CEZAR RIBEIRO, UNESP; NEIVA MARIA ROBALDO GUEDES, INSTITUTO MAMEDE: PESQUISA AMBIENTAL E ECOTURISMO; NICOLAY LEME DA CUNHA, UFMS; OTÁVIO FROEHLICH, UFMS; PATRÍCIA MEDICI, IPÊ; PAULINO BARROSO MEDINA JUNIOR, UNIDERP; PAULO ROBSON DE SOUZA, UFMS; RAFAEL PENEDO FERREIRA, UFMS; REINALDO FRANCISCO FERREIRA LOURIVAL, UFGD; ROBERTO MACEDO GAMARRA, UFMS; RODRIGO BORGHEZAN, UNIDERP; RUDI RICARDO LAPS, UFMS; SABINE BORGES DA ROCHA, UFMS; SAMUEL DULEBA, UFMS; SIMONE MAMEDE, UFMS; SUZANA CUNHA ESCAPINATI, BIOMA - CONSULTORIA EM TURISMO E MEIO AMBIENTE; THOMAZ RICARDO FAVRETO SINANI, UFMS; VANDA LUCIA FERREIRA, UFMS; WALFRIDO MORAES TOMAS, CPAP; YZEL RONDON SÚAREZ, UEMS; FÁBIO DE OLIVEIRA ROQUE, UFMS. |
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Título: |
Incorporating biodiversity expert knowledge in landscape conservation planning: a case study involving the Pantanal. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 5., 2014, Campo Grande, MS. Anais... São José dos Campos: INPE, 2014. |
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Páginas: |
p. 544-553. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
Geopantanal 2014. |
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Conteúdo: |
We developed a framework to incorporate biodiversity information about target species based on expert knowledge into landscape metrics in order to subsidize conservation planning. Four steps make up our framework:
1) biodiversity expert consults, 2) processing and organizing the information, 3) validating the information and 4) incorporating biodiversity data into landscape metrics. As a case study, we applied this framework in the Upper Paraguai basin in the limits of Mato Grosso do Sul state. This is a part of an ongoing effort to incorporate biodiversity in the Zoneamento Ecológico Econômico of Mato Grosso do Sul state. Based on systematically collected data from biodiversity expert questionnaires, ours results helped to fill species-level knowledge gaps concerning functional connectivity and matrix permeability at the landscape scale and provide clear target species parameters for the generation of conservation scenarios. This approach meets the need for spatially-explicit species based targets and recognize the importance for biodiversity for sustainable territorial planning. |
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Palavras-Chave: |
Conectividade funcional; Conhecimento de especialistas; Conservation planning; Ecological Economic Zoning; Espécies alvo; Expert knowledge; Functional connectivity; Matrix; Planejamento de conservação; Target species; Zoneamento Ecológico Econômico. |
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Thesagro: |
Bacia Hidrográfica; Matriz; Permeabilidade. |
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Thesaurus NAL: |
permeability; Watersheds. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 04219naa a2201117 a 4500
001 2008959
005 2015-02-20
008 2014 bl --- 0-- u #d
100 1 $aSUGAI, L. S. M.
245 $aIncorporating biodiversity expert knowledge in landscape conservation planning$ba case study involving the Pantanal.
260 $c2014
300 $ap. 544-553.$c1 CD-ROM.
500 $aGeopantanal 2014.
520 $aWe developed a framework to incorporate biodiversity information about target species based on expert knowledge into landscape metrics in order to subsidize conservation planning. Four steps make up our framework: 1) biodiversity expert consults, 2) processing and organizing the information, 3) validating the information and 4) incorporating biodiversity data into landscape metrics. As a case study, we applied this framework in the Upper Paraguai basin in the limits of Mato Grosso do Sul state. This is a part of an ongoing effort to incorporate biodiversity in the Zoneamento Ecológico Econômico of Mato Grosso do Sul state. Based on systematically collected data from biodiversity expert questionnaires, ours results helped to fill species-level knowledge gaps concerning functional connectivity and matrix permeability at the landscape scale and provide clear target species parameters for the generation of conservation scenarios. This approach meets the need for spatially-explicit species based targets and recognize the importance for biodiversity for sustainable territorial planning.
650 $apermeability
650 $aWatersheds
650 $aBacia Hidrográfica
650 $aMatriz
650 $aPermeabilidade
653 $aConectividade funcional
653 $aConhecimento de especialistas
653 $aConservation planning
653 $aEcological Economic Zoning
653 $aEspécies alvo
653 $aExpert knowledge
653 $aFunctional connectivity
653 $aMatrix
653 $aPlanejamento de conservação
653 $aTarget species
653 $aZoneamento Ecológico Econômico
700 1 $aCOSTA-PEREIRA, R.
700 1 $aQUINTEIRO, J. M. O.
700 1 $aTORRECILHA, S.
700 1 $aERIKSSON, A.
700 1 $aNUNES, A. P.
700 1 $aKEUROGHLIAN, A.
700 1 $aARAUJO, A. C.
700 1 $aPARANHOS FILHO, A. C.
700 1 $aDESBIEZ, A. L. J.
700 1 $aPOTT, A.
700 1 $aSANTOS, C. F.
700 1 $aFERREIRA, C. M. M.
700 1 $aRIBEIRO, D. B.
700 1 $aEATON, D. P.
700 1 $aSCREMIN-DIAS, E.
700 1 $aCORRÊA, EL. C.
700 1 $aBURKHARDT, E.
700 1 $aFISCHER, E.
700 1 $aAYRES, F. M.
700 1 $aMARTINS, F. I.
700 1 $aALVES, F. M.
700 1 $aSEVERO-NETO, F.
700 1 $aSOUZA, F. L.
700 1 $aPAULA, G. A. DE.
700 1 $aSOUZA, G. N. de
700 1 $aSEIXAS, G. H. F.
700 1 $aDALPONTI, G.
700 1 $aBARROS, G. M. de
700 1 $aGRACIOLLI, G.
700 1 $aGIMÊNES JUNIOR, H.
700 1 $aILHA, I. M. N.
700 1 $aSUGAI, J. L. M. M.
700 1 $aLONGO, J. M.
700 1 $aSABINO, J.
700 1 $aCORDEIRO, L. M.
700 1 $aNASCIMENTO, L. N.
700 1 $aTENCATT, L. F. C.
700 1 $aBUENO, M. L.
700 1 $aRODRIGUES, M. E.
700 1 $aBENITES, M.
700 1 $aSILVA, M.
700 1 $aGODOI, M. N.
700 1 $aSILVEIRA, M.
700 1 $aDELATORRE, M.
700 1 $aRIBEIRO, M. C.
700 1 $aGUEDES, N. M. R.
700 1 $aCUNHA, N. L. da
700 1 $aFROEHLICH, O.
700 1 $aMEDICI, P.
700 1 $aMEDINA JUNIOR, P. B.
700 1 $aSOUZA, P. R. de
700 1 $aFERREIRA, R. P.
700 1 $aLOURIVAL, R. F. F.
700 1 $aGAMARRA, R. M.
700 1 $aBORGHEZAN, R.
700 1 $aLAPS, R. R.
700 1 $aROCHA, S. B. da
700 1 $aDULEBA, S.
700 1 $aMAMEDE, S.
700 1 $aESCAPINATI, S. C.
700 1 $aSINANI, T. R. F.
700 1 $aFERREIRA, V. L.
700 1 $aTOMAS, W. M.
700 1 $aSÚAREZ, Y. R.
700 1 $aROQUE, F. de O.
773 $tIn: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 5., 2014, Campo Grande, MS. Anais... São José dos Campos: INPE, 2014.
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